Reverse microbial etiology: A research field for predicting and preventing emerging infectious diseases caused by an unknown microorganism

Xu, Jianguo

doi:10.1016/j.jobb.2018.12.005

Cited by 8 publications

(5 citation statements)

References 27 publications

(21 reference statements)

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“…Additionally, O. pekingense was first isolated from the activated sludge by Liu in 2008(Liu et al, 2008, which may be regarded as a causative agent for causing ocular infection (Borsali et al, 2011). These studies were in line with our previously proposed reverse microbial etiology (Xu, 2019), which was to predict and prevent a potential emerging infectious disease caused by an unknown microorganism. Moreover, Ornithinimicrobium may be used as one of the potential markers to distinguish Graves' disease and Hashimoto's thyroiditis from the healthy population for participating in the occurrence and development of the disease by regulating purine metabolism and pyrimidine metabolism (Zhao et al, 2022).…”

Section: Introductionsupporting

confidence: 88%

Morphological and genomic characteristics of two novel actinomycetes, Ornithinimicrobium sufpigmenti sp. nov. and Ornithinimicrobium faecis sp. nov. isolated from bat faeces (Rousettus leschenaultia and Taphozous perforates)

Huang

Zhang²,

Tao³

et al. 2023

Front. Cell. Infect. Microbiol.

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Four Gram-staining-positive, aerobic, non-motile, circle-shaped bacteria were isolated from the faeces of bats (Rousettus leschenaultia and Taphozous perforates) collected from Guangxi autonomous region (E106°49′20″, N22°20′54″) and Yunnan province (E102°04′39″, N25°09′10″) of South China. Strains HY006T and HY008 shared highly 16S rRNA gene sequence similarity to those of Ornithinimicrobium pratense W204T (99.3%) and O. flavum CPCC 203535T (97.3%), while the strains HY1745 and HY1793T were closest to the type strains O. ciconiae H23M54T (98.7%), O. cavernae CFH 30183T (98.3%), and O. murale 01-Gi-040T (98.1%). Furthermore, when compared to the other members of the genus Ornithinimicrobium, the digital DNA-DNA hybridization and average nucleotide identity values of the four novel strains were within the ranges of 19.6–33.7% and 70.6–87.4%, respectively, both of which were below the respective recommended cutoff values of 70.0% and 95–96%. Significantly, strain HY006T was resistant to chloramphenicol and linezolid whereas strain HY1793T was resistant to erythromycin, clindamycin (intermediately), and levofloxacin (intermediately). The main cellular fatty acids (>20.0%) of our isolates were iso-C15:0 and iso-C16:0. Strains HY006T and HY1793T contained ornithine as the diagnostic diamino acid, also along with the alanine, glycine and glutamic acid in their cell wall. Based on phylogenetic, chemotaxonomic and phenotypic analyses, these four strains could be classified as two novel species of the genus Ornithinimicrobium, for which the names Ornithinimicrobium sufpigmenti sp. nov. and Ornithinimicrobium faecis sp. nov. are proposed. The type strains are HY006T (=CGMCC 1.16565T =JCM 33397T) and HY1793T (=CGMCC 1.19143T =JCM 34881T), respectively.

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Section: Introductionsupporting

confidence: 88%

Morphological and genomic characteristics of two novel actinomycetes, Ornithinimicrobium sufpigmenti sp. nov. and Ornithinimicrobium faecis sp. nov. isolated from bat faeces (Rousettus leschenaultia and Taphozous perforates)

Huang

Zhang²,

Tao³

et al. 2023

Front. Cell. Infect. Microbiol.

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“…In June 2018, a routine surveillance of viruses carried by sandflies was conducted in Shanxi Province (112°5′–114°4′ E, 37°40′–38°31′ N), central China [ 9 , 10 ]. Sandflies were captured in light traps (Wentaitai MM200 traps, Guangzhou Changsheng Chemical Technology Service Co., Ltd., Guangzhou, China) and transported to the laboratory in Beijing on dry ice.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Identification of a Novel Phlebovirus, Hedi Virus, from Sandflies Collected in China

Fan

Hou

et al. 2021

Viruses

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We report the isolation of a newly recognized phlebovirus, Hedi virus (HEDV), from Phlebotomus chinensis sandflies collected in Shanxi Province, China. The virus’ RNA is comprised of three segments. The greatest amino acid sequence similarity of the three gene segments between this virus and previously recognized phleboviruses is 40.85–63.52%, and the RNA-dependent RNA polymerase (RdRp) amino acid sequence has the greatest similarity (63.52%) to the Rift Valley fever virus (RVFV) ZH-548 strain. Phylogenetic analysis of the amino acid sequence of the virus RdRp indicated that HEDV is close to RVFV and distinct from other phleboviruses, forming its own evolutionary branch. We conclude that it is necessary to increase the monitoring of phleboviruses carried by sandflies in China.

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“…Several pre-identifications of pathogens have been reported to cause human infections. Reverse microbial etiology is one way to study emerging infectious, and the methods of this study followed previous presented procedures ( Xu, 2019 ). When emerging infectious diseases affect plants, primarily commercial crops, it can cause substantial economic losses.…”

Section: Discussionmentioning

confidence: 99%

A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducks

Tao

Yang

et al. 2023

Front. Cell. Infect. Microbiol.

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The species in the genus Erwinia are Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped. Most species in the genus Erwinia are phytopathogens. Also, Erwinia persicina was involved in several human infections. Based on the reverse microbial etiology principles, it is worth analyzing the pathogenicity of species in this genus. In this study, we isolated and sequenced two species of Erwinia. Phylogenetic, phenotypic, biochemical, and chemotaxonomic analyses were performed to identify its taxonomy position. The virulence tests on plant leaves and pear fruits were used to identify the plant pathogenicity of two species of Erwinia. Bioinformatic methods predicted the possible pathogenic determinants based on the genome sequence. Meanwhile, adhesion, invasion, and cytotoxicity assays on RAW 264.7 cells were applied to identify animal pathogenicity. We isolated two Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped strains from the feces of ruddy shelducks in the Tibet Plateau of China, designated J780T and J316. Distinct phylogenetic, genomic, phenotypic, biochemical, and chemotaxonomic characters of J780T and J316 identified they were novel species and belonged to the genus Erwinia, for which the name Erwinia sorbitola sp. nov. was proposed, the type strain was J780T (= CGMCC 1.17334T = GDMCC 1.1666T = JCM 33839T). Virulence tests showed blight and rot on the leaves and pear fruits confirmed Erwinia sorbitola sp. nov. was a phytopathogen. Predicted gene clusters of motility, biofilm formation, exopolysaccharides, stress survival, siderophores, and Type VI secretion system might be the causes of pathogenicity. In addition, predicted polysaccharide biosynthesis gene clusters on the genome sequence, and the high capacity for adhesion, invasion, and cytotoxicity to animal cells confirmed it has pathogenicity on animals. In conclusion, we isolated and identified a novel phytopathogen Erwinia sorbitola sp. nov. in ruddy shelducks. A predefined pathogen is beneficial for preventing from suffering potential economic losses caused by this new pathogen.

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Reverse microbial etiology: A research field for predicting and preventing emerging infectious diseases caused by an unknown microorganism

Cited by 8 publications

References 27 publications

Morphological and genomic characteristics of two novel actinomycetes, Ornithinimicrobium sufpigmenti sp. nov. and Ornithinimicrobium faecis sp. nov. isolated from bat faeces (Rousettus leschenaultia and Taphozous perforates)

Morphological and genomic characteristics of two novel actinomycetes, Ornithinimicrobium sufpigmenti sp. nov. and Ornithinimicrobium faecis sp. nov. isolated from bat faeces (Rousettus leschenaultia and Taphozous perforates)

Isolation and Identification of a Novel Phlebovirus, Hedi Virus, from Sandflies Collected in China

A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducks

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