Reverse transcriptase of rat liver associated with the endogenous retrovirus related to the mouse intracisternal A-particles

Salganik, R. I.; Tomsons, V. P.; Пыринова, Г Б; Korokhov, Nikolay; Киселева, Елена; Khristolyubova, N. B.

doi:10.1016/0006-291x(85)91829-7

Cited by 13 publications

(4 citation statements)

References 8 publications

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“…Though still hypothetical, this view is compatible with a body of published data: First, it is well known that retrotransposons and endogenous retroviruses are highly expressed in poorly differentiated, highly proliferating cells, both normal (i.e. early embryo cells) and pathological (tumors) (reviewed in Spadafora, 2004), while being repressed or minimally expressed in terminally differentiated, nonpathological tissues (Kiessling and Goulian, 1979;Salganick et al, 1985;Medstrand and Blomberg, 1993). Similarly, LTR (long terminal repeat) enhancers of human endogenous retroviruses have been shown to be activated in stem/progenitor cells of adult tissues, while being inactive in adult somatic cells (Pi et al, 2004).…”

Section: Rt-mediated Inheritancesupporting

confidence: 69%

Retrotransposons, reverse transcriptase and the genesis of new genetic information

Sciamanna

Vitullo

Curatolo

et al. 2009

Gene

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Section: Rt-mediated Inheritancesupporting

confidence: 69%

Retrotransposons, reverse transcriptase and the genesis of new genetic information

Sciamanna

Vitullo

Curatolo

et al. 2009

Gene

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“…Consistent with this, high expression of the cellular RT gene has been detected in transformed cells (Deragon et al, 1990;Martin, 1991;Martin and Branciforte, 1993) compared to the low level detected in nonmalignant, differentiated cells (Kiessling and Goluian, 1979;Salganick et al, 1985;Medstrand and Blomberg, 1993;Banerjee and Thampan, 2000). …”

Section: Introductionmentioning

confidence: 66%

Exposure of normal and transformed cells to nevirapine, a reverse transcriptase inhibitor, reduces cell growth and promotes differentiation

et al. 2003

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Endogenous, nontelomeric reverse transcriptase (RT) is encoded by two classes of repeated elements: retrotransposons and endogenous retroviruses. Expression of RT-coding genes is generally repressed in differentiated nonpathological tissues, yet is active in the mammalian germ line, embryonic tissues and tumor cells. Nevirapine is a non-nucleoside RT inhibitor with a well-characterized inhibitory activity on RT enzymes of retroviral origin. Here, we show that nevirapine is also an effective inhibitor of the endogenous RT in murine and human cell lines. In addition, progenitor and transformed cells undergo a significant reduction in the rate of cell growth upon exposure to nevirapine. This is accompanied by the onset of differentiation, as depicted in F9 and C2C7 progenitor cells cultures in which nevirapine triggers the expression of differentiation-specific markers. Consistent with this, an extensive reprogramming of cell cycle gene expression was depicted in nevirapine-treated F9 cultures. Furthermore, nevirapine exposure rescued the differentiation block present in acute myeloid leukemia (AML) cell lines and primary blasts from two AML patients, as indicated by morphological, functional and immunophenotypic assays. The finding that an RT inhibitor can modulate cell proliferation and differentiation suggests that RT may represent a novel target in the development of therapeutical approaches to neoplasia.

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“…Purification of intracellular retroviral particles usually gives rise to very low amounts of purified structures [11,23,25,31]. We thus employed the best producer selected, 5 AdC treated BHK21 cl.…”

Section: Discussionmentioning

confidence: 99%

“…We employed various buffers, conditions ofcentrifugation and extraction of cytoplasmic material reported for the purification of other types of intracisternal particles [11,23,25]. All our prepa-t04 J.…”

Section: Purification Of Lrp Coresmentioning

confidence: 99%

Biological and molecular studies on Syrian hamster intracisternal R-type particles

Lesser

Bittoun

Emanoil-Ravier

et al. 1995

Archives of Virology

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Retrovirus-like intracisternal R-type particles (IRP) are structures present in Syrian hamster (Mesocricetus auratus) cells cultured in vitro where they appear either spontaneously or under chemical induction conditions. We have tested several chemical inducers and ten different cell lines, looking for the best IRP induction conditions. BHK21 cl. 13 showed the highest inducibility one day after a 24 h treatment with 1 microgram/ml of 5-aza-2'-deoxycytidine. Using detergent treatments and sucrose gradients, we obtained semi-purified IRP cores. A 7.2 kb RNA associated with the core fraction was revealed by hybridization with total Syrian hamster genomic DNA, but not with Syrian hamster intracisternal A particle (IAP) specific probes. This suggests that the IRP genes are distinct from IAP ones.

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Reverse transcriptase of rat liver associated with the endogenous retrovirus related to the mouse intracisternal A-particles

Cited by 13 publications

References 8 publications

Retrotransposons, reverse transcriptase and the genesis of new genetic information

Retrotransposons, reverse transcriptase and the genesis of new genetic information

Exposure of normal and transformed cells to nevirapine, a reverse transcriptase inhibitor, reduces cell growth and promotes differentiation

Biological and molecular studies on Syrian hamster intracisternal R-type particles

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