Reversed-phase C18 high-performance liquid chromatography of gibberellins GA3 and GA1

Castillo, G. Del; Martinez, Servando

doi:10.1016/s0021-9673(97)00427-5

Cited by 18 publications

(14 citation statements)

References 11 publications

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“…The ␤-tubulin gene from F. fujikuroi, amplified with primers 5Ј-CCGGTGCTGGAAACAACTG-3Ј and 5Ј-CGAGGACCTGGTCGACAAG T-3Ј, (16) Chemical determinations. For gibberellic acid (GA 3 ) determinations, 1-ml samples were acidified to pH 2.5 with 10 M HCl, cleaned by filtration through 0.22-m-pore Millipore filters, and run in an HPLC device (Agilent Technologies, Santa Clara, CA) with 30% methanol and 10 mM H 3 PO 4 (pH 3) (14). Concentrations were obtained from the peaks at 206 nm of commercial GA 3 (Sigma, Saint Louis, MO) used as a standard.…”

Section: Methodsmentioning

confidence: 99%

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Rodríguez-Ortiz

Limón

Ávalos

2009

Appl Environ Microbiol

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The fungus Fusarium fujikuroi (Gibberella fujikuroi MP-C) produces metabolites of biotechnological interest, such as gibberellins, bikaverins, and carotenoids. Gibberellin and bikaverin productions are induced upon nitrogen exhaustion, while carotenoid accumulation is stimulated by light. We evaluated the effect of nitrogen availability on carotenogenesis in comparison with bikaverin and gibberellin production in the wild type and in carotenoid-overproducing mutants (carS). Nitrogen starvation increased carotenoid accumulation in all strains tested. In carS strains, gibberellin and bikaverin biosynthesis patterns differed from those of the wild type and paralleled the expression of key genes for both pathways, coding for geranylgeranyl pyrophosphate (GGPP) and kaurene synthases for the former and a polyketide synthase for the latter. These results suggest regulatory connections between carotenoid biosynthesis and nitrogen-controlled biosynthetic pathways in this fungus. Expression of gene ggs1, which encodes a second GGPP synthase, was also derepressed in the carS mutants, suggesting the participation of Ggs1 in carotenoid biosynthesis. The carS mutations did not affect genes for earlier steps of the terpenoid pathway, such as fppS or hmgR. Light induced carotenoid biosynthesis in the wild type and carRA and carB levels in the wild-type and carS strains irrespective of nitrogen availability.

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Section: Methodsmentioning

confidence: 99%

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Rodríguez-Ortiz

Limón

Ávalos

2009

Appl Environ Microbiol

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“…Table 4 indicates the average of 4 results obtained by SIA with the tubular detector (TE), the analysis of the same samples through direct potentiometry in batch conditions with the previously described electrode 7 and with an independent method based on the HPLC technique. 2 As can be observed the proposed procedure gave results with lower relative errors (< 0.5%) relatively to those provided by conventional units (< 3.3%) when both are compared with the HPLC method. The improvement of the analytical results is more significant when the complexity of sample increases, such as the case of Berelex formulation.…”

Section: Analytical Applicationsmentioning

confidence: 66%

“…In the real sample analysis by the HPLC method proposed by Martinez et al, 2 the mobile phase was made up of a degasified mixture of 300.0 mL of methanol and 700.0 mL of a 10 -2 mol L -1 phosphoric acid solution whose pH was previously adjusted to 3.0 with 0.1 mol L -1 KOH. This eluent was also used for the preparation of a 1 mg mL -1 gibberellic acid standard solution and as solvent of the remaining less-concentrated solutions.…”

Section: Introductionmentioning

confidence: 99%

Determination of gibberellic acid by sequential injection analysis using a potentiometric detector based on Mn(III)-porphyrin with improved characteristics

Santos¹,

Couto²,

Araújo³

et al. 2004

J. Braz. Chem. Soc.

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Descreve-se a determinação de ácido giberélico em promotores de crescimento usados na agricultura, por análise de injecção sequencial e detecção potentiométrica. , declive de -64,5 ± 1,6 mV/dec e melhores coeficientes de selectividade potenciométrica (log K pot ) do que os obtidos com eléctrodos convencionais. Na análise de amostras comercializadas em Portugal obtiveram-se desvios padrão de 0,01 e 0,4 (n= 4) e um ritmo de amostragem de 30 amostras/hora. This work describes the determination of gibberellic acid in growth promoters formulations used in agriculture by sequential injection analysis and potentiometric detection. The potentiometric detectors with improved characteristics used a PVC membrane prepared with Mn(III)tetraphenylporphyrin-Cl as electroactive specie. Different membranes formulated with several ionic additives were compared in order to select the most suitable one concerning slope, response time, reproducibility and selectivity. A membrane containing 6 wt% of manganese tetraphenylporphyrin-Cl [(Mn(TPP)Cl], 27 wt% of PVC, 66 wt% of dibutylphthalate (DBP), as mediator solvent, and 1 wt% of sodium tetraphenylborate, as anionic additive, was chosen for analytical applications. When coupled to a SIA system the tubular electrode presented a linear range between 5×10 -4 mol L -1 and 8×10 -3 mol L -1 , a slope of -64.5 ± 1.6 mV/dec and lower potentiometric selectivity coefficients (log K pot ) than those obtained with the conventional electrodes. Standard deviation of 0.01 and 0.4 (n= 4) and a sampling rate of 30 samples/hour were obtained in the analysis of real samples.

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“…6 Many of them respond to environmental stimuli such as photoperiod, temperature, water and nutrition supply. 7 Taking gibberellic acid as an example, it promotes seed germination, stem elongation, flowering and cone production, and retards leaf and fruit senescence. It also induces de novo synthesis of numerous enzymes.…”

Section: Introductionmentioning

confidence: 99%

Separation of Plant Hormones from Biofertilizer by Capillary Electrophoresis Using a Capillary Coated Dynamically with Polycationic Polymers

Jiang

Yue

2006

ANAL. SCI.

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Reversed-phase C18 high-performance liquid chromatography of gibberellins GA3 and GA1

Cited by 18 publications

References 11 publications

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Determination of gibberellic acid by sequential injection analysis using a potentiometric detector based on Mn(III)-porphyrin with improved characteristics

Separation of Plant Hormones from Biofertilizer by Capillary Electrophoresis Using a Capillary Coated Dynamically with Polycationic Polymers

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