Reversed-phase high-performance liquid chromatographic determination of salbutamol in rabbit plasma

Kurosawa, Nahoko; Morishima, Seiji; Owada, Eiji; Ito, Keiji

doi:10.1016/s0378-4347(00)83366-6

Cited by 27 publications

(4 citation statements)

References 7 publications

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“…Alternative low cost methods for the measurement of salbutamol and terbutaline have utilized high performance liquid chromatography (HPLC) with electroche-* Author to whom correspondence should be addiessed. mica1 and fluorescence detection (Edholm et al, 1982;Williams et al, 1982;Bergquist and Edholm, 1983;Edholm et al, 1984;Emm et al, 1988;Kurosawa et al, 1984;Ong et uf., 1989). Many of these have been multicolumn methods, often with on-line solid phase extraction.…”

Section: Lntroductionmentioning

confidence: 99%

Measurement of terbutaline and salbutamol in plasma by high performance liquid chromatography with fluorescence detection

McCarthy

Atwal

Sykes³

et al. 1993

Biomedical Chromatography

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A method is described for the determination of terbutaline and salbutamol in plasma from patients given maximal therapy for brittle asthma. The analytes were isolated by solid phase extraction on alkali-treated Bond-Elut, unmodified, silica columns and measured by high performance liquid chromatography with fluorescence detection (excitation wavelength 200 nm). The limits of detection for a 1 mL sample containing salbutamol and terbutaline were 1 microgram/L and 2.5 micrograms/L, respectively. The intra-assay precision (CV) for samples containing 25 micrograms/L was 3.6 and 5.0% respectively. This method was applied to the measurement of terbutaline in samples from patients given continuous infusions of the drug to assess whether this treatment might result in toxicity.

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Section: Lntroductionmentioning

confidence: 99%

Measurement of terbutaline and salbutamol in plasma by high performance liquid chromatography with fluorescence detection

McCarthy

Atwal

Sykes³

et al. 1993

Biomedical Chromatography

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“…-HPTLC -Salbutamol -Beclomethasone -Inhalation product Several methods are reported for simultaneous determination of components of fixed-dose combination products containing SAL and other drug substance in the form of pulmonary product. [14][15][16] The HPLC methods with fluorescent 17 and electrochemical 18,19 detection and LC-MS/MS method 20 are reported for determination of SAL in biological fluids such as plasma and urine. The HPLC methods are reported for assay of BD in bulk drug in Indian Pharmacopoeia, 21 British Pharmacopoeia 22 and European Pharmacopoeia.…”

Section: Introductionmentioning

confidence: 99%

A Sensitive High-Performance Thin Layer Chromatography Method for Simultaneous Determination of Salbutamol Sulphate and Beclomethasone Dipropionate from Inhalation Product

Patel¹,

Parmar²

2018

Pharm Sci

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Background: A sensitive high-performance thin layer chromatographic (HPTLC) method was developed for simultaneous determination of beclomethasone dipropionate and salbutamol sulphate from inhalation product. Methods: Chromatographic separation was achieved on aluminium plates pre-coated with silica gel G60 F254 as the stationary phase and methanol:ethyl acetate:toluene:ammonia (3:1:3:0.15) as the mobile phase. The densitometric evaluation was carried out at 232 nm. The developed method was validated as per the ICH Q2 (R1) guidelines. Robustness of the proposed method was assessed using experimental design approach, and results were analyzed graphically. The results of sample analysis by proposed HPTLC method and reported HPLC method were statistically compared. Results: The Rf value of salbutamol sulphate and beclomethasone dipropionate was found to be 0.38±0.02 and 0.72±0.02, correspondingly. The response in terms of peak area was linear over the concentration range of 100-500 ng/spot and 200-1000 ng/spot for beclomethasone dipropionate and salbutamol sulphate, individually, with the regression coefficient values greater than 0.99 for both the drugs. The limit of detection and limit of quantification for beclomethasone dipropionate were found to be 27 ng/spot and 84 ng/spot, respectively and for salbutamol sulphate were 40 ng/spot and 112 ng/spot, respectively. The proposed method was found to be statistically insignificant when compared to the reported method. Conclusion: The proposed method is robust, sensitive, accurate and precise. The method can be applied for the simultaneous estimation of beclomethasone dipropionate and salbutamol sulphate from inhalation product.

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“…Various methods for the isolation of albuterol from biological matrices have been reported including ion-pair extraction, 5 solid phase extraction cartridges (SPE), [6][7][8][9] SPE/ ion pair, 10 and immunoaffinity chromatography, 11 all with recoveries ranging from about 84 to 99%. Highest extraction efficiency was the result of immunoaffinity chromatography but the antibody necessary for this method is not commercially available.…”

mentioning

confidence: 99%

Determination of the enantiomers of albuterol in human and canine plasma by enantioselective high-performance liquid chromatography on a teicoplanin-based chiral stationary phase

1998

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Reversed-phase high-performance liquid chromatographic determination of salbutamol in rabbit plasma

Cited by 27 publications

References 7 publications

Measurement of terbutaline and salbutamol in plasma by high performance liquid chromatography with fluorescence detection

Measurement of terbutaline and salbutamol in plasma by high performance liquid chromatography with fluorescence detection

A Sensitive High-Performance Thin Layer Chromatography Method for Simultaneous Determination of Salbutamol Sulphate and Beclomethasone Dipropionate from Inhalation Product

Determination of the enantiomers of albuterol in human and canine plasma by enantioselective high-performance liquid chromatography on a teicoplanin-based chiral stationary phase

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