Reversed-phase high-performance liquid chromatography for simultaneous determination of prostaglandins E2, A2 and B2

A liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry (LC-FAIMS-MS/MS) semi-quantitative method was developed for the simultaneous determination of prostaglandin (PG) E2, PGD2, PGF(2alpha), 6-keto-PGF(1alpha), and thromboxane (TX) B2. Diluted samples containing these prostanoids and their tetra-deuterium-substituted internal standards were analyzed by LC followed by either selected reaction monitoring (LC-SRM) or FAIMS and selected reaction monitoring (LC-FRM). FAIMS reduced background noise, separated the isobaric ions PGE2 and PGD2, and separated dynamically interchanging TXB2 anomers. This is the first report of the separation of interconverting anomers by FAIMS. Generally, the LC-FRM chromatograms were more selective than the LC-SRM chromatograms. Its potential was demonstrated by analysis of prostanoids in guinea pig lumbar spinal cord homogenate.

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Simultaneous analysis of prostanoids using liquid chromatography/high‐field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry

Kapron

Jin

Mauriala

et al. 2006

Rapid Comm Mass Spectrometry

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Effect of 2-hydroxypropyl-β-cyclodextrin on the stability of prostaglandin E2 in solution

Song

Lee

1994

International Journal of Pharmaceutics

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Reversed-phase high-performance liquid chromatography for simultaneous determination of prostaglandins E2, A2 and B2

Cited by 2 publications

References 10 publications

Simultaneous analysis of prostanoids using liquid chromatography/high‐field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry

Simultaneous analysis of prostanoids using liquid chromatography/high‐field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry

Effect of 2-hydroxypropyl-β-cyclodextrin on the stability of prostaglandin E2 in solution

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