Reversed-phase ion-pair chromatography coupled to electrospray ionisation mass spectrometry by on-line removal of the counter-ions

Forngren, Benita H.; Samskog, Jenny; Gustavsson, Sven; Tyrefors, Niklas; Markides, Karin E.; Långström, Bengt

doi:10.1016/s0021-9673(99)00526-9

Cited by 23 publications

(16 citation statements)

References 21 publications

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“…Commonly, the mobile phase buffers used together with electrospray ionization mass spectrometric detection are volatile, as a nonvolatile buffer may cause problems with remaining impurities on the interface 11,15 and with ion suppression. 16 When a mobile phase of low pH is needed for the separation, formic acid may hence be a logical choice as a buffer additive.…”

Section: Results and Discussion Ion Signal And Selector Concentrationmentioning

confidence: 99%

“…In addition to the choice of buffer, mobile phase additives may also cause problems in the ionization process. [10][11][12][13][14][15][16] The chiral selector used in this study is, for example, nonvolatile and its presence in the mobile phase may therefore influence the intensity of the mass spectrometric signal in a nonlinear way. Mass spectra for lasalocid with and without analytes present are shown in Figure 2.…”

Section: Results and Discussion Ion Signal And Selector Concentrationmentioning

confidence: 99%

“…3 It is of interest to be able to use mass spectrometry as a detection technique also for analysis of chiral compounds, 9 due to its complementary selectivity, low detection limits, and structural information. The performance of the mass spectrometer with electrospray ionization is, however, usually lowered when nonvolatile components are used in the mobile phase 10 like phosphate buffer salts and ion pairs 11,12 or chiral selector additives. 13,14 The purpose of the present study was to evaluate the opportunities for combining liquid chromatographic separation, where PGC is carrying a dynamically held chiral selector, with mass spectrometric detection.…”

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Use of an adsorbed chiral selector in capillary LC‐MS

et al. 2002

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Lasalocid was utilized as a chiral selector adsorbed on porous graphitic carbon. Important parameters were identified for the use of the chiral selector in capillary liquid chromatography combined with MS detection. The influence on both retention and enantioselectivity as well as mass spectrometric performance was studied at lasalocid concentrations of up to 12 mg/l (20 microM). Moreover, the stability of retention factors and enantioselectivities was also evaluated at low selector concentration of 1 mg/l (2 microM). The results show that in order to optimize the MS performance the selector concentration should be kept at low microM. The chromatographic performance at reduced selector concentration (1 mg/l = 2 microM) was studied over a 10-day period, showing satisfactory enantioselectivity and stable performance concerning enantioselectivity and retention.

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Section: Results and Discussion Ion Signal And Selector Concentrationmentioning

confidence: 99%

Section: Results and Discussion Ion Signal And Selector Concentrationmentioning

confidence: 99%

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confidence: 99%

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Use of an adsorbed chiral selector in capillary LC‐MS

et al. 2002

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“…Ion-pair reagents such as heptafluorobutyric acid (HFBA) have been used to improve the retention and separation of CQ and MQ on C18 column [8,11]. The use of ion-pair reagents, however, could easily contaminate the mass spectrometer, resulting in the need for frequent cleaning [15][16][17]. Hydrophilic interaction liquid chromatography (HILIC) column has been recently used to solve the separation problem [18].…”

Section: Introductionmentioning

confidence: 99%

An improved method for analyzing chlormequat and mepiquat in source waters by solid-phase extraction and liquid chromatography–mass spectrometry

Jin

et al. 2010

Analytica Chimica Acta

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a b s t r a c tA liquid chromatography-mass spectrometry (LC-MS) method for the identification and quantification of chlormequat (CQ) and mepiquat (MQ) in source waters with high sensitivity and specificity was established using WCX cartridges (150 mg/6 mL) for pre-concentration of the samples and using the CAPCELL PAK CR 1:4 (2.0 mm × 150 mm 5 m, SCX:C18 = 1:4) column containing strong cationic exchange resins and C18 for separation. The method could solve the problem for pre-concentrating ionic compounds from water samples and avoid the MS instrument fouling problem accompanied with the use of ion-pair reagents. After the optimization of analytical conditions, quantification was achieved in the positive electrospray ionization mode using selected ion monitoring. The samples were analyzed with multi-channel mode with quantification performed at 30 V cone voltage to ascertain the sensitivity and qualitative analysis performed at 60 V cone voltage simultaneously. The method detection limits (MDLs) of CQ and MQ in source water were determined to be 14 and 22 ng L −1 . Finally, the method was used to quantify CQ and MQ in several source waters, which gave a level ranging from below the quantitation limit to 28 ng L −1 .

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“…Either cationic or anionic membrane suppressors for ion chromatography-MS with an ion-spray interface were reported by Conboy et al [22], whereas Forngren et al [23] removed octane sulfonic acid and sodium dodecyl sulfonate with an anion-exchanger placed between the separation column and the MS interface for the analysis of biopterin and guanidine.…”

Section: Introductionmentioning

confidence: 99%

Analysis of sulfonated compounds by reversed-phase ion-pair chromatography—Mass spectrometry with on-line removal of non-volatile tetrabutyl ammonium ion-pairing agents

Socher¹,

Nussbaum²,

Rißler³

et al. 2001

Chromatographia

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SummaryAn ion-pair reversed-phase high performance liquid chromatography method with tetrabutyl ammonium salts as ion-pairing agents and mass spectrometric detection has been developed for sulfonated compounds with special focus on structural identification.A cation exchange suppressor cartridge placed bef,,veen the UV detector and the ion source of the mass spectrometer completely removed the non-volatile ion-pairing agent resulting in excellent conditions for both electrospray ioniziation and atmospheric pressure chemical ioniziation. Peak broadening caused bythe dead volume of the suppressor is negligible.The application range of this method is demonstrated by the structural identification of individual compounds within a complex mixture of model substances consisting of sulfonated aromatics, textile dyes and cletergents with as many as four sulfonic acid groups, all largely differing from each other in their structural properties. The influence of the ion-pairing agent's counter ion on retention behaviour is also discussed.

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Reversed-phase ion-pair chromatography coupled to electrospray ionisation mass spectrometry by on-line removal of the counter-ions

Cited by 23 publications

References 21 publications

Use of an adsorbed chiral selector in capillary LC‐MS

Use of an adsorbed chiral selector in capillary LC‐MS

An improved method for analyzing chlormequat and mepiquat in source waters by solid-phase extraction and liquid chromatography–mass spectrometry

Analysis of sulfonated compounds by reversed-phase ion-pair chromatography—Mass spectrometry with on-line removal of non-volatile tetrabutyl ammonium ion-pairing agents

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