Reversed-phase pressurized planar electrochromatography and planar chromatography of acetylsalicylic acid, caffeine, and acetaminophen

Pressurized planar electrochromatography (PPEC) is a separating technique in which an electric field is applied to force the mobile phase movement through a porous media (electroosmotic effect). High separation efficiency, fast separations and changes in separation selectivity in comparison to liquid chromatography, especially thin layer chromatography (planar chromatography, TLC), are features of this technique. Constructional methodological challenges to PPEC are obstacles to its development and application in laboratory practice. In this article, an attempt to overcome the challenges related to device construction and sample application/injection is described. The introduced device enables both prewetting of the adsorbent layer and electrochromatogram development with a single PPEC device. It also enables simultaneous application/injection of six samples on a chromatographic plate in a stream of the mobile phase (on-line application/injection). In addition, the PPEC chamber was equipped with a thermostat. The device is characterized by an impressive throughput in comparison to the other planar technique, TLC/HPTLC. Although the developed device still needs improvement, it is, in our opinion, a considerable step toward possible automation of this planar separation technique.

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“…Then, 4-mm-wide margins were produced with sealant solution on the whole periphery of the adsorbent layer of each chromatographic plate [12]. …”

Section: Methodsmentioning

confidence: 99%

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

et al. 2013

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“…Wiszkidenszky et al, for example, have developed a purity test for allylestrenol bulk drug substance and tablet by means of OPLC analysis. The method has been validated and enables marking all impurities of allylestrenol except one isomer [45]. Furthermore, researchers from India have developed a validated method for separation and quantification of colchicine from tubers of Gloriosa superba.…”

Section: Fundamentals Of Oplc and Ppec And Their Application In Drug mentioning

confidence: 99%

An underestimated technique. Does pressurized and pressure-assisted capillary electrochromatography have potential in drug and pharmacological-active compounds analysis?

Trzebiatowski

Gwarda

Dzido

2019

Current Issues in Pharmacy and Medical Sciences

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Pharmaceutical analysis guarantees patient safety all over the world. Thus, continuous development of existing analytical techniques is still very important. Pressurized capillary electrochromatography and pressure-assisted capillary electrochromatography are hybrid separation techniques that combine the selectivity of liquid chromatography and the high separation efficiency of capillary electrophoresis. They use a smaller amount of reagents and samples, hence, reducing the total cost of analysis. Therefore they have found application in a number of pharmaceutical and biomedical analysis. This review article focuses on the use and importance of pressurized and pressure-assisted capillary electrochromatography in pharmaceutical and biomedical analysis, taking into account types of detectors and capillaries used. Despite the fact that pressurized capillary electrochromatography and pressure assisted capillary electrochromatography offer many possibilities and have been available for over a dozen years, they are still underdevelopment and not fully explored.

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“…After that, the plate was dried in air and, next, in the oven at 105 °C for 60 min and left in desiccator for cooling. Detailed procedure description of the margin preparation is described in our previous papers [19, 20, 25]. The plates were used for experiments within 1 day.…”

Section: Ppec Experimentsmentioning

confidence: 99%

Influence of the Modifier Type and its Concentration on Electroosmotic Flow of the Mobile Phase in Pressurized Planar Electrochromatography

et al. 2014

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The aim of this work was to find a relationship between electroosmotic flow (EOF) velocity of the mobile phase in pressurized planar electrochromatography (PPEC) and physicochemical properties like zeta potential, dielectric constant, and viscosity of the mobile phase as well as its composition. The study included different types of organic modifiers (acetonitrile, methanol, ethanol, acetone, formamide, N-methylformamide and N,N-dimethylformamide) in the full concentration range. In all experiments, chromatographic glass plates HPTLC RP-18 W from Merck (Darmstadt) were used as a stationary phase. During the study we found that there is no linear correlation between EOF velocity of the mobile phase and single variables such as zeta potential or dielectric constant or viscosity. However, there is quite strong linear correlation between EOF velocity of the mobile phase and variable obtained by multiplying zeta potential of the stationary phase–mobile phase interface, by dielectric constant of the mobile phase solution and dividing by viscosity of the mobile phase. Therefore, it could be concluded that the PPEC system fulfilled the Helmholtz–Smoluchowski equation.

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Reversed-phase pressurized planar electrochromatography and planar chromatography of acetylsalicylic acid, caffeine, and acetaminophen

Cited by 32 publications

References 30 publications

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

An underestimated technique. Does pressurized and pressure-assisted capillary electrochromatography have potential in drug and pharmacological-active compounds analysis?

Influence of the Modifier Type and its Concentration on Electroosmotic Flow of the Mobile Phase in Pressurized Planar Electrochromatography

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