Reversible associations between human plasma fibronectin and fibrinogen γγ’ heterodimer observed by high pressure size exclusion chromatography and dynamic light scattering

Fabian, Frank; Ismail, Ayman; Wang, Ou; Lei, Yu; Velander, William H.

doi:10.1016/j.ab.2020.113701

Cited by 5 publications

(2 citation statements)

References 48 publications

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“…This complex could be dissociated by passing through a gelatin affinity column where the pFN was absorbed, and γγ′F1 passed through the affinity column. We also found that the dissociated γγ′F1 and pFN could quickly re-associate at room temperature [ 62 ]. The high purity of the compositions used in this paper consisting of pFN, γγF1, γγ′F1, γγ′F1:pFN and a 1:1 mixture of γγF1 and pFN (γγF + pFN) were confirmed with reduced SDS-PAGE ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Jara

Wang

Prado

et al. 2020

Bioactive Materials

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Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1), and 10% has a heterodimeric pair of γ and more acidic γ′ chains (γγ′F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ′F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were decorated with pFN nanoclusters (termed γγ′F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of “pFN droplets” (termed γγF1+pFN fibrin). The γγ′F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ′F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ′F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1+pFN fibrin. Relative to γγF1+pFN fibrin, mouse dermal wounds that were sealed by γγ′F1:pFN fibrin exhibited accelerated and enhanced healing. This study suggests that a 3D pFN presentation on a fibrin matrix promotes wound healing.

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Section: Resultsmentioning

confidence: 99%

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Jara

Wang

Prado

et al. 2020

Bioactive Materials

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“…). We also found that the dissociated γγ'F1 and pFN could quickly re-associate at room temperature 55 . The high purity of the compositions used in this paper consisting of pFN, γγF1, γγ'F1, γγ'F1:pFN and a 1:1 mixture of γγF1 and pFN (γγF+pFN) were confirmed with reduced SDS-PAGE ( Fig.…”

Section: Resultsmentioning

confidence: 59%

Three-Dimensional (3D) Fibronectin Nano-Array Presented on Fibrin Matrix Accelerates Mice Skin Wound Healing

Wang

et al. 2020

Preprint

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Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1) and 10% has a heterodimeric pair of γ and more acidic γ' chains (γγ'F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ'F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were wrapped with periodic 200-300 nm wide pFN nanobands (termed γγ'F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of "pFN droplets" (termed γγF1+pFN fibrin). The γγ'F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ'F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ'F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1+pFN fibrin. Relative to γγF1+pFN fibrin, mouse dermal wounds that were sealed by γγ'F1:pFN fibrin exhibited accelerated and enhanced healing. This study suggests that a 3D pFN nano-array presented on a fibrin matrix can promote wound healing.

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