2016
DOI: 10.1021/acs.analchem.6b02512
|View full text |Cite
|
Sign up to set email alerts
|

Reversible G Protein βγ9 Distribution-Based Assay Reveals Molecular Underpinnings in Subcellular, Single-Cell, and Multicellular GPCR and G Protein Activity

Abstract: Current assays to measure the activation of G protein coupled receptors (GPCRs) and G proteins are time-consuming, indirect, and expensive. Therefore, an efficient method which directly measures the ability of a ligand to govern GPCR-G protein interactions can help to understand the molecular underpinnings of the associated signaling. A live cell imaging-based approach is presented here to directly measure ligand-induced GPCR and G protein activity in real time. The number of active GPCRs governs G protein het… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
32
0

Year Published

2017
2017
2022
2022

Publication Types

Select...
8
1
1

Relationship

7
3

Authors

Journals

citations
Cited by 27 publications
(32 citation statements)
references
References 44 publications
0
32
0
Order By: Relevance
“…As an extension to our previous model (37), reactions for Gβγ effector activation in a multi-Gγ containing cell were modeled, to decipher how a diverse group of Gγ types in a cell controls Gβγ effector activities. The optimized model (variation 3) encompasses reactions in Fig.…”
Section: Data-guided Computational Modeling Of Gγ Subtypes Driven Sigmentioning
confidence: 99%
“…As an extension to our previous model (37), reactions for Gβγ effector activation in a multi-Gγ containing cell were modeled, to decipher how a diverse group of Gγ types in a cell controls Gβγ effector activities. The optimized model (variation 3) encompasses reactions in Fig.…”
Section: Data-guided Computational Modeling Of Gγ Subtypes Driven Sigmentioning
confidence: 99%
“…Visual transduction requires the activation of G-protein heterotrimers by light-activated opsins, and cells in the retina primarily use farnesylated Gg types (Gg1 and Gg9) in heterotrimers. Since statins greatly perturb the PM localization of the farnesylated Gg types, we examined whether statins reduce Gbg translocation (which is considered a measure of heterotrimer activation) (Senarath et al, 2016) in adult retinal pigment epithelial-19 (ARPE-19) cells. Gg-subtype expression profile of ARPE-19 cells was determined using quantitative real-time PCR (qRT-PCR).…”
Section: Fluvastatin Significantly Impairs Heterotrimer Activation Anmentioning
confidence: 99%
“…To examine whether opsins and G proteins can be activated exclusively at the LE, the G␥9 assay was used (39). This assay employs the translocation of mCherry G␥9 (mCh-␥9) from the PM to internal membranes (IMs), generated by activated GPCRs.…”
Section: Oa Of Blue Opsin For Subcellular G Protein Activation and DImentioning
confidence: 99%