Reversibly Photoswitching Upconversion Nanoparticles for Super‐Sensitive Photoacoustic Molecular Imaging

Rao, Jianghong; Liu, Cheng; Zheng, Xianchuang; Dai, Tingting; Wang, Shuangfeng; Chen, Xian; Chen, Bing; Sun, Tianying; Wang, Feng; Chu, Steven

doi:10.1002/ange.202116802

Cited by 7 publications

(10 citation statements)

References 44 publications

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“…Following the clear demonstrations of reversible photoconversion proteins for providing a high SNR and contrast-tobackground ratio (CBR), Liu et al recently presented a novel strategy for developing reversibly photoswitchable nanoprobes by combining upconversion nanoparticles (UCNPs) with photochromic small molecules within an amphiphilic polymer coating (Figure 6a,b). 309 The nanoprobe they developed had two main components: Small molecules (dithienylethenecontaining β-diketone, 3ThacacH) and UCNPs. First, photoswitchable small molecules were switched "on" by 365 nm ultraviolet (UV) light (defined as the transition from an open form to a closed form) and "off" by 640 nm red light illumination (λ 2 in Figure 6a, defined as the transition from the closed form to the open form).…”

Section: Reversibly Photoswitchable Chromophoresmentioning

confidence: 99%

“…Following the clear demonstrations of reversible photoconversion proteins for providing a high SNR and contrast-to-background ratio (CBR), Liu et al recently presented a novel strategy for developing reversibly photoswitchable nanoprobes by combining upconversion nanoparticles (UCNPs) with photochromic small molecules within an amphiphilic polymer coating (Figure a,b) . The nanoprobe they developed had two main components: Small molecules (dithienylethene-containing β-diketone, 3ThacacH) and UCNPs.…”

Section: Challenge 1: Snr or Contrastmentioning

confidence: 99%

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Recent Advances in Contrast-Enhanced Photoacoustic Imaging: Overcoming the Physical and Practical Challenges

et al. 2023

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For decades now, photoacoustic imaging (PAI) has been investigated to realize its potential as a niche biomedical imaging modality. Despite its highly desirable optical contrast and ultrasonic spatiotemporal resolution, PAI is challenged by such physical limitations as a low signal-to-noise ratio (SNR), diminished image contrast due to strong optical attenuation, and a lower-bound on spatial resolution in deep tissue. In addition, contrast-enhanced PAI has faced practical limitations such as insufficient cell-specific targeting due to low delivery efficiency and difficulties in developing clinically translatable agents. Identifying these limitations is essential to the continuing expansion of the field, and substantial advances in developing contrast-enhancing agents, complemented by high-performance image acquisition systems, have synergistically dealt with the challenges of conventional PAI. This review covers the past four years of research on pushing the physical and practical challenges of PAI in terms of SNR/contrast, spatial resolution, targeted delivery, and clinical application. Promising strategies for dealing with each challenge are reviewed in detail, and future research directions for next generation contrast-enhanced PAI are discussed.

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Section: Reversibly Photoswitchable Chromophoresmentioning

confidence: 99%

Section: Challenge 1: Snr or Contrastmentioning

confidence: 99%

Recent Advances in Contrast-Enhanced Photoacoustic Imaging: Overcoming the Physical and Practical Challenges

et al. 2023

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Section: Nir‐to‐uv Upconversion For Bioapplicationsmentioning

confidence: 99%

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confidence: 99%

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Tuning Near‐Infrared‐to‐Ultraviolet Upconversion in Lanthanide‐Doped Nanoparticles for Biomedical Applications

Wei

Zheng

Zhang

et al. 2022

Advanced Optical Materials

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converting NIR light to UV and vis light owing to several merits such as low toxicity, [11] high stability, [12] and minimal photo bleaching. [13] Ln-doped UCNPs can be readily prepared by several complementary methods such as co-precipitation, [14] thermal decomposition, [15] and thermal injection. [16] The as-synthesized nanoparticles can also be readily modified by silica, [17] mesoporous silica, [18] and hydrophilic molecules [19] with good biocompatibility. Importantly, UCNPs can easily convert multiwavelength NIR light to tunable UV light by doping different lanthanide ions [20] such as Yb 3+ , Nd 3+ , Er 3+ , Tm 3+ , and Gd 3+ . [21] Compared with visible emissions, the high-energy UV photons enable effective simulation of various photoactivatable systems, thus, making these UCNPs attractive nanoplatforms for light-controlled diagnosis and therapy in biomedicine. [22] This review focuses on the recent developments of NIR-to-UV UCNPs and the relevant applications in biomedicine. In Section 2, we survey the strategies for tuning UV emissions in Ln-doped UCNPs by excitations of NIR lasers at different wavelengths. In Section 3, we highlight the emerging applications of NIR-to-UV UCNPs in biomedical diagnosis and therapy. Strategies for NIR-to-UV UpconversionTo realize NIR-to-UV upconversion emission, a sensitizer with a sufficient absorption cross-section in the NIR region is essential. The selection of appropriate sensitizer co-doped along with the activator through an efficient energy transfer process could realize strong UV upconversion emissions under different excitation wavelengths (Figure 1). For example, Yb 3+ is an ideal sensitizer for 980 nm excitation because of its larger absorption cross-section at around 980 nm than other lanthanide ions due to the 2 F 7/2 → 2 F 5/2 transition. [23] Additionally, the 2 F 5/2 → 2 F 7/2 transition of Yb 3+ well matches electronic transitions in typical upconverting activators (e.g., Er 3+ , Tm 3+ , and Ho 3+ ) that facilitates energy transfer upconversion (ETU). [24] Nd 3+ is particularly suitable for use as a sensitizer to absorb shorter-wavelength laser due to its multiple NIR excitation bands at 730, 808, and 865 nm, corresponding to transitions from the 4 I 9/2 state to the 4

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Multiplane Spectroscopic Whole‐Body Photoacoustic Computed Tomography of Small Animals In Vivo

Yang,

Choi,

Kim

et al. 2024

Laser & Photonics Reviews

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To understand complex biological process and disease progression, it is essential to comprehensively track biodynamics across entire organisms. The invaluable tracking tool, photoacoustic computed tomography (PACT), provides insights into structural, functional, and molecular aspects of living tissues. However, current small‐animal PACT systems suffer from low temporal/spatial resolution and a restricted field of view, and they are limited in the biological information they can provide, hindering research on whole‐body biodynamics. Here, it is demonstrated a continuous rotary‐scanning PACT system for rapid monitoring of various parameters within the relatively large torso of a small animal. In this PACT system, a hemispherical transducer array is continuously rotated at high speed, enabling a 3D scan of an entire mouse body in just 54 s, with a spatial resolution of 172–212 µm. The rapid rotary scanning allows us to not only image whole‐body structures but also to monitor pharmacokinetics and changes in hemoglobin oxygen saturation in living animals. This approach holds great promise for advancing the understanding of in vivo biological dynamics, opening up a new avenue of preclinical research in areas such as metabolic diseases and drug delivery.

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Reversibly Photoswitching Upconversion Nanoparticles for Super‐Sensitive Photoacoustic Molecular Imaging

Cited by 7 publications

References 44 publications

Recent Advances in Contrast-Enhanced Photoacoustic Imaging: Overcoming the Physical and Practical Challenges

Recent Advances in Contrast-Enhanced Photoacoustic Imaging: Overcoming the Physical and Practical Challenges

Tuning Near‐Infrared‐to‐Ultraviolet Upconversion in Lanthanide‐Doped Nanoparticles for Biomedical Applications

Multiplane Spectroscopic Whole‐Body Photoacoustic Computed Tomography of Small Animals In Vivo

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