Review: Microfluidics technologies for blood-based cancer liquid biopsies

Sun, Yuxi; Haglund, Thomas A.; Rogers, Aaron J.; Ghanim, Asem; Sethu, Palaniappan

doi:10.1016/j.aca.2017.12.050

Cited by 87 publications

(52 citation statements)

References 228 publications

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“…Cancer, a leading cause of death around the world and, in fact, the most important cause of death and morbidity in Europe and United States following cardiovascular diseases mostly appears as solid tumours of the breast, lung, bronchus, prostate, colon, rectum and bladder . It is nowadays well‐known that early detection, even before symptoms appear, of some of the most common cancer types, such as breast, colorectal and cervical cancers, increases the chance of cure, together with reliable evaluation of the disease progression and response to treatment .…”

Section: Introductionmentioning

confidence: 99%

Disposable Amperometric Immunosensor for the Determination of the E‐Cadherin Tumor Suppressor Protein in Cancer Cells and Human Tissues

et al. 2018

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This paper describes the results obtained in the development of the first electrochemical immunosensor described to date for the detection of E‐cadherin (E‐cad) protein, a relevant biomarker of prognosis and metastasis in cancer, based on the use of magnetic microcarriers (MBs) and amperometric transduction at screen‐printed carbon electrodes (SPCEs). Thus, the determination of E‐cad protein involved the use of two specific antibodies against this protein (one of them labelled with HRP) in a sandwich configuration onto HOOC‐MBs. The magnetic bioconjugates were captured onto SPCEs and the amperometric transduction was performed using the H2O2/hydroquinone (HQ) system. Under optimal conditions, this bioplatform demonstrated a wide linear concentration range (0.50–25 ng mL−1) and a detection limit as low as 0.16 ng mL−1, well below the optimal cut‐off level for the E‐cad protein (defined as 10,000 ng mL−1 for soluble E‐cad levels in serum). The developed sensor also showed a good reproducibility among measurements with seven different sensors constructed in the same manner (RSD, 5.4 %), stability for more than 15 days and good specificity towards other proteins commonly found on biological samples. The applicability of this simple handling bioplatform for the direct determination of this protein in cell lysates with different metastatic potential and extracts from paraffined‐embedded human colorectal cancer tissues of different grade were also demonstrated.

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Section: Introductionmentioning

confidence: 99%

Disposable Amperometric Immunosensor for the Determination of the E‐Cadherin Tumor Suppressor Protein in Cancer Cells and Human Tissues

et al. 2018

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“…The spatial and temporal delivery of biomolecules in these systems attempts to control the fate of cellular processes with many of these systems utilizing pre-equilibrated media or gas channels to achieve desired oxygenation (Beachley et al, 2015;Grist et al, 2012;Grist et al, 2015;Samorezov and Alsberg, 2015). A standard of systematic oxygenation has not yet been established in these systems, so the implementation of a method of physiological oxygenation to 3D cultures that also allows for high-throughput scalability is therefore highly desirable (Brennan et al, 2014;Place et al, 2017;Sun et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Spatial control of oxygen delivery to 3D cultures alters cancer cell growth and gene expression

Wulftange

Garmendia‐Cedillos

et al. 2019

Preprint

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Commonly used monolayer cancer cell cultures fail to provide a physiologically relevant environment in terms of oxygen delivery. Here, we describe a three-dimensional bioreactor system where cancer cells are grown in Matrigel in modified 6-well plates. Oxygen is delivered to the cultures through a polydimethylsiloxane (PDMS) membrane at the bottom of the wells, with microfabricated PDMS pillars to control oxygen delivery. The plates receive 3% oxygen from below and 0% oxygen at the top surface of the media, providing a gradient of 3% to 0% oxygen. We compared growth and transcriptional profiles for cancer cells grown in Matrigel in the bioreactor, 3D cultures grown in 21% oxygen, and cells grown in a standard hypoxia chamber at 3% oxygen. Additionally, we compared gene expression of conventional 2D monolayer culture and 3D Matrigel culture in 21% oxygen. We conclude that controlled oxygen delivery may provide a more physiologically relevant 3D system.

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“…Furthermore, circulating microvesicles could exist in abundance in cancer patients (13 trillion per ml of plasma). In fact, exosomes are found to increase by a few folds in cancer patients [1], and could provide crucial information that complements the biodata derived from CTCs and ctDNAs. Exosomal contents have thus emerged as promising biomarkers comparable or even superior to the other analytes.…”

mentioning

confidence: 99%

“…Attracted by its clinical significance, many researchers have invested enormous efforts in ensuring capture with high purity and high throughput. As most CTCs are distinctly larger than other blood cells, numerous physical size-based microfluidic technologies have been successfully developed and commercialized in the past decade [1]. Recent efforts are now leaning towards the design of special geometries within the microchannels to isolate single CTCs into different compartments.…”

mentioning

confidence: 99%

“…Notably, this makes it possible to capture and isolate tumor-derived cells from peripheral blood. For example, circulating tumor cells (CTCs) in blood were found to originate either from the primary tumor or secondary metastatic site [1], and the enumeration of CTCs alone could be used to correlate prognosis. Attracted by its clinical significance, many researchers have invested enormous efforts in ensuring capture with high purity and high throughput.…”

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confidence: 99%

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