Review of cell and particle trapping in microfluidic systems

Nilsson, Johan; Evander, Mikael; Hammarström, Björn; Laurell, Thomas

doi:10.1016/j.aca.2009.07.017

Cited by 483 publications

(368 citation statements)

References 110 publications

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“…Cells are immobilized in certain regions of the chip after being separated from the flow by different mechanical barriers or obstacles. 32,33 The obstacle or barrier dimensions are similar to the sizes of the cells to be captured. There have been a few ways to trap cells by hydrodynamic trapping: ͑i͒ front-trapping, ͑ii͒ side-trapping, ͑iii͒ gravity trapping, and ͑iv͒ microvortex trapping.…”

Section: Hydrodynamic Trappingmentioning

confidence: 87%

“…For this application, gradients of the magnetic field were achieved using a pin holder and used for a 3D cell culture array. 33 Ho et al 95 initially formed random arrangement of multicellular spheroids of HeLa cells after labeling them with paramag-netic particles. These spheroids were patterned rapidly with magnetic fields.…”

Section: Magnetophoretic Trappingmentioning

confidence: 99%

“…The resultant magnetic force used in microfluidic devices is in the range of 2-1000 pN. 33 Depending on the properties of the cell, there are three main types of magnetic trapping: ͑i͒ using the diamagnetic properties of cells, 84 ͑ii͒ using paramagnetic properties of cells, 85 and ͑iii͒ using surface chemistry to attach the cell to magnetic particles. 86 The main advantage of the first method is that it is applicable to any diamagnetic particle as long as its magnetic susceptibility is different from that of the medium and no chemical or physical modification of the surface is required.…”

Section: Magnetophoretic Trappingmentioning

confidence: 99%

See 2 more Smart Citations

Exploitation of physical and chemical constraints for three-dimensional microtissue construction in microfluidics

Choudhury

Iliescu

et al. 2011

Biomicrofluidics

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There are a plethora of approaches to construct microtissues as building blocks for the repair and regeneration of larger and complex tissues. Here we focus on various physical and chemical trapping methods for engineering three-dimensional microtissue constructs in microfluidic systems that recapitulate the in vivo tissue microstructures and functions. Advances in these in vitro tissue models have enabled various applications, including drug screening, disease or injury models, and cellbased biosensors. The future would see strides toward the mesoscale control of even finer tissue microstructures and the scaling of various designs for high throughput applications. These tools and knowledge will establish the foundation for precision engineering of complex tissues of the internal organs for biomedical applications.

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Section: Hydrodynamic Trappingmentioning

confidence: 87%

Section: Magnetophoretic Trappingmentioning

confidence: 99%

Section: Magnetophoretic Trappingmentioning

confidence: 99%

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Exploitation of physical and chemical constraints for three-dimensional microtissue construction in microfluidics

Choudhury

Iliescu

et al. 2011

Biomicrofluidics

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“…84,85 Early work on cell electrical measurements dates back to the 1910s, [86][87][88] the foundation for interpreting the electrical properties of cells, where the cell is modeled as a spherical cytoplasm surrounded by a thin dielectric membrane. 89,90 Generally, the electrical properties of a plasma membrane are affected by the membrane morphology, lipid bilayer composition and thickness, and embedded proteins.…”

Section: Electrical Characterization Techniquesmentioning

confidence: 99%

Recent advances in microfluidic techniques for single-cell biophysical characterization

et al. 2013

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“…Isolation of individual live cells from suspensions shows promise for applications of highthroughput single-cell and even organelle microanalysis. A variety of methods, including flow cytometry and microfluidic and array-based platforms, allow cell targeting based on fluorescent tags or even metabolic activity (Nilsson et al, 2009;Suzuki et al, 2009).…”

Section: Sampling Strategies For Microanalysismentioning

confidence: 99%

Small-Volume Analysis of Cell–Cell Signaling Molecules in the Brain

Romanova

Aerts

Croushore

et al. 2013

Neuropsychopharmacol

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Modern science is characterized by integration and synergy between research fields. Accordingly, as technological advances allow new and more ambitious quests in scientific inquiry, numerous analytical and engineering techniques have become useful tools in biological research. The focus of this review is on cutting edge technologies that aid direct measurement of bioactive compounds in the nervous system to facilitate fundamental research, diagnostics, and drug discovery. We discuss challenges associated with measurement of cell-to-cell signaling molecules in the nervous system, and advocate for a decrease of sample volumes to the nanoliter volume regimen for improved analysis outcomes. We highlight effective approaches for the collection, separation, and detection of such small-volume samples, present strategies for targeted and discovery-oriented research, and describe the required technology advances that will empower future translational science.

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Review of cell and particle trapping in microfluidic systems

Cited by 483 publications

References 110 publications

Exploitation of physical and chemical constraints for three-dimensional microtissue construction in microfluidics

Exploitation of physical and chemical constraints for three-dimensional microtissue construction in microfluidics

Recent advances in microfluidic techniques for single-cell biophysical characterization

Small-Volume Analysis of Cell–Cell Signaling Molecules in the Brain

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