Review of phage display: A jack-of-all-trades and master of most biomolecule display

Song, Brenda Pei Chui; Ch'ng, Angela Chiew Wen; Lim, Theam Soon

doi:10.1016/j.ijbiomac.2023.128455

Cited by 10 publications

(3 citation statements)

References 193 publications

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“…Furthermore, phage display technology, allowing us to expose virtually any peptides on the surface of phage virions when fused to one of coat proteins [25], has revolutionized the biotechnological search for compounds able to bind to any specific target molecules. This technology is useful in a wide spectrum of applications, such as improving medical diagnostics, searching for novel drugs, developing effective vaccines, inactivating toxins, producing novel materials (including nanomaterials), and others [26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Bacteriophages—Dangerous Viruses Acting Incognito or Underestimated Saviors in the Fight against Bacteria?

Podlacha,

Węgrzyn,

Węgrzyn

2024

IJMS

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The steadily increasing number of drug-resistant bacterial species has prompted the search for alternative treatments, resulting in a growing interest in bacteriophages. Although they are viruses infecting bacterial cells, bacteriophages are an extremely important part of the human microbiota. By interacting with eukaryotic cells, they are able to modulate the functioning of many systems, including the immune and nervous systems, affecting not only the homeostasis of the organism, but potentially also the regulation of pathological processes. Therefore, the aim of this review is to answer the questions of (i) how animal/human immune systems respond to bacteriophages under physiological conditions and under conditions of reduced immunity, especially during bacterial infection; (ii) whether bacteriophages can induce negative changes in brain functioning after crossing the blood–brain barrier, which could result in various disorders or in an increase in the risk of neurodegenerative diseases; and (iii) how bacteriophages can modify gut microbiota. The crucial dilemma is whether administration of bacteriophages is always beneficial or rather if it may involve any risks.

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Section: Introductionmentioning

confidence: 99%

Bacteriophages—Dangerous Viruses Acting Incognito or Underestimated Saviors in the Fight against Bacteria?

Podlacha,

Węgrzyn,

Węgrzyn

2024

IJMS

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“…Phage display was pioneered by Dr. George Smith at the University of Missouri in 1985 [ 1 ]. The technology is a combinatorial technique that employs the use of an assembled library of filamentous bacteriophages, in which the DNA has been genetically modified, resulting in the fusion of a foreign peptide or antibody to the N-terminal end of one of the viral coat proteins [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. Such phage libraries provide researchers with a vast pool from which polypeptide-based ligands that exhibit high affinity and specificity towards a target antigen can be selected.…”

Section: Introductionmentioning

confidence: 99%

Improving Pharmacokinetics of Peptides Using Phage Display

Asar,

Newton-Northup,

Soendergaard

2024

Viruses

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Phage display is a versatile method often used in the discovery of peptides that targets disease-related biomarkers. A major advantage of this technology is the ease and cost efficiency of affinity selection, also known as biopanning, to identify novel peptides. While it is relatively straightforward to identify peptides with optimal binding affinity, the pharmacokinetics of the selected peptides often prove to be suboptimal. Therefore, careful consideration of the experimental conditions, including the choice of using in vitro, in situ, or in vivo affinity selections, is essential in generating peptides with high affinity and specificity that also demonstrate desirable pharmacokinetics. Specifically, in vivo biopanning, or the combination of in vitro, in situ, and in vivo affinity selections, has been proven to influence the biodistribution and clearance of peptides and peptide-conjugated nanoparticles. Additionally, the marked difference in properties between peptides and nanoparticles must be considered. While peptide biodistribution depends primarily on physiochemical properties and can be modified by amino acid modifications, the size and shape of nanoparticles also affect both absorption and distribution. Thus, optimization of the desired pharmacokinetic properties should be an important consideration in biopanning strategies to enable the selection of peptides and peptide-conjugated nanoparticles that effectively target biomarkers in vivo.

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“…Soon after its birth in 1985, following a short lag period [ 1 , 2 ], the phage display technology enjoyed a tremendous exponential growth, demonstrating its competence and performance in solving numerous challenging problems in material science, bioengineering, and medicine, including cancer research [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ] ( Figure 1 ). This Special Issue aims to illustrate the promise of phage display as a universal research tool in cancer research.…”

Section: Introductionmentioning

confidence: 99%