Review on Citrinin: Production, Effect of Some Plant Extracts and Gene Involved in its Biosynthesis

Abou-Zeid, Alaa Mostafa

doi:10.4172/2165-784x.1000113

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…It has been commonly used as a model PKS for evaluation of heterologously expression system such as S. cerevisiae [ 3 , 11 ], S. coelicolo r [ 12 ], E. coli [ 13 ], tobacco [ 14 ]. Mycotoxin citrinin was originally isolated from Penicillium citrinum and now produced by a variety of other fungi [ 15 ]. It damages human health and usually accompanies with pigment production in Monascus spp., and gives rise to wide attention.…”

Section: Introductionmentioning

confidence: 99%

Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

et al. 2013

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BackgroundPolyketides are one of the most important classes of secondary metabolites and usually make good drugs. Currently, heterologous production of fungal polyketides for developing a high potential industrial application system with high production capacity and pharmacutical feasibility was still at its infancy. Pichia pastoris is a highly successful system for the high production of a variety of heterologous proteins. In this work, we aim to develop a P. pastoris based in vivo fungal polyketide production system for first time and evaluate its feasibility for future industrial application.ResultsA recombinant P. pastoris GS115-NpgA-ATX with Aspergillus nidulans phosphopantetheinyl transferase (PPtase) gene npgA and Aspergillus terrus 6-methylsalicylic acid (6-MSA) synthase (6-MSAS) gene atX was constructed. A specific compound was isolated and idenified as 6-MSA by HPLC, LC-MS and NMR. Transcription of both genes were detected. In 5-L bioreactor, the GS115-NpgA-ATX grew well and produced 6-MSA quickly until reached a high value of 2.2 g/L by methanol induction for 20 hours. Thereafter, the cells turned to death ascribing to high concentration of antimicrobial 6-MSA. The distribution of 6-MSA changed that during early and late induction phase it existed more in supernatant while during intermediate stage it mainly located intracellular. Different from 6-MSA production strain, recombinant M. purpureus pksCT expression strains for citrinin intermediate production, no matter PksCT located in cytoplasm or in peroxisomes, did not produce any specfic compound. However, both npgA and pksCT transcripted effectively in cells and western blot analysis proved the expression of PPtase. Then the PPTase was expressed and purified, marked by fluorescent probes, and reacted with purified ACP domain and its mutant ACPm of PksCT. Fluoresence was only observed in ACP but not ACPm, indicating that the PPTase worked well with ACP to make it bioactive holo-ACP. Thus, some other factors may affect polyketide synthesis that include activities of the individual catalytic domains and release of the product from the synthase of PksCT.ConclusionsAn efficient P. pastoris expression system of fungal polyketides was successfully constructed. It produced a high production of 6-MSA and holds potential for future industrial application of 6-MSA and other fungal polyketides.

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Section: Introductionmentioning

confidence: 99%

Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

et al. 2013

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“…There have been several reports on the isolation of bioactive metabolites, and the investigation of the ethyl acetate extracts from the broth and mycelia of P. citrinum has led to the extraction of nearly eighteen metabolites [11]. Bioactivity of citrinin possesses antibiotic, bacteriostatic, antifungal and antiprotozoal properties [12]. Citrinin was isolated from a Penicillium janczewskii strain found to inhibit the root-rot pathogen Heterobasidion annosum.…”

Section: Application Of Citrininmentioning

confidence: 99%

“…The optimum conditions for citrinin production were temperature 35 °C, pH 7.0 salinty20ppt, carbon source-3% glucose, Nitrogen source-0.5% sodium nitrite, 3% rice bran Compared to commercially available carbon source (glucose), rice bran gave higher production of citrinin. About 1.50µg/ml of citrinin was produced at optimized condition [12]. Likewise, another researcher Kaur et al [25] carried out solid state fermentation for the production of pigments by using broken wheat (0.996 OD).…”

Section: Production Of Citrininmentioning

confidence: 99%

Review on (Yelow Pigment) Citrinin

Vasanthabharathi¹

2018

JBB

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Mazumde et al. [15] observed Minimum Inhibitory Concentration (MIC) against some gram positive strains viz. Staphylococcus aureus, Bacillus pumilus, Bacillus subtillis, Bacillus cereus, Klebsiella pneumoniae, Streptococcus pneumoniae, Lactobacillus arabinosus and gram negative strains E. Coli, Shigella dysenteriae, shigella sonnei, shigella boydii, Salmonella typhimurium, Proteus mirabilis and Vibrio cholerae by using citrinin. Antioxidant JBIR-124 [16], Cytotoxic penicitrinols C and E, topo IIαinhibitory tricitrinol B and the cytotoxic dicitrinone B [17]. The isolation of five polyketides including two benzopyranones, one isochroman

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Recent advances in reconstructing microbial secondary metabolites biosynthesis in Aspergillus spp.

Wang

Chen

et al. 2018

Biotechnology Advances

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Review on Citrinin: Production, Effect of Some Plant Extracts and Gene Involved in its Biosynthesis

Cited by 8 publications

References 20 publications

Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

Review on (Yelow Pigment) Citrinin

Recent advances in reconstructing microbial secondary metabolites biosynthesis in Aspergillus spp.

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