Review on Different Methods to Assess the Antioxidant Activity of Some Common Plants of Indian Traditional Medicine

Dey, Samiran; Saxena, Pallavi

doi:10.22270/jddt.v1i1.27

Cited by 2 publications

(3 citation statements)

References 7 publications

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“…These are the best methods for analyzing antioxidant and free radical scavenging activity in the studied samples. 17 The DPPH assay revealed a better IC50 value at a lesser concentration of 0.23 μg/mL for V. vinifera extract, followed by Z. officinale and P. nigrum extracts. The B. nigra and A. cepa extracts showed the IC50 value for DPPH at ∼2 μg/mL.…”

Section: Resultsmentioning

confidence: 91%

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Novel Cell-Based Assay to Investigate Monoacylglycerol Acyltransferase 2 Inhibitory Activity Using HIEC-6 Cell Line

et al. 2021

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The dietary triacylglycerol (TAG) gets absorbed and accumulated in the body through the monoacylglycerol (MAG) pathway, which plays a major role in obesity and related disorders. The main enzyme of this pathway, monoacylglycerol acyltransferase 2 (MGAT2), is considered as a potential target for developing antiobesity compounds. Hence, there is a need for in vitro cell-based assays for screening the potential leads for MGAT2 inhibitors. Because of synthetic inhibitor's side effects, there is an increased interest in natural extracts as potential leads. Hence, we have optimized a 2-MAG-induced TAG accumulation inhibitory cell-based assay to screen natural extracts using the HIEC-6 cell line. A concentration-dependent TAG accumulation was observed when the HIEC-6 cells were fed with exogenous 2-MAG. The TAG accumulation was confirmed by in situ BODIPY staining and was quantified. However, no TAG accumulation was seen when the cells were fed with exogenous DAG or TAG, suggesting MGAT2mediated MAG uptake and its conversion to TAG. We demonstrated the utility of this assay by screening five different plant-based aqueous extracts. These extracts showed various inhibition levels (25% to 30%) of 2-MAG-induced TAG accumulation in the HIEC-6. The MGAT2 inhibitory potential of these extracts was confirmed by an in vitro MGAT2 assay. This cell-based assay adds a new methodology for screening, developing, and evaluating MGAT2 inhibitors for addressing obesity and related disorders.

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Section: Resultsmentioning

confidence: 91%

“…The antioxidant assays for different extracts were performed by DPPH, ABTS, and FRAP methods and are presented in Table . These are the best methods for analyzing antioxidant and free radical scavenging activity in the studied samples . The DPPH assay revealed a better IC50 value at a lesser concentration of 0.23 μg/mL for V.…”

Section: Resultsmentioning

confidence: 97%

Novel Cell-Based Assay to Investigate Monoacylglycerol Acyltransferase 2 Inhibitory Activity Using HIEC-6 Cell Line

et al. 2021

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“…In phenolics, flavonoids are one of the naturally distributed secondary metabolites in plants. The flavonoids exhibit an anti-oxidative effect, which results in the inhibition of lipid peroxidation to scavenge free radicals and active oxygen, chelate iron ions, and inactivate lipoxygenase [34]. It has been found that the presence of higher phenols and flavonoids in plants contributes to their antioxidant potential.…”

Section: Total Flavonoid Contentmentioning

confidence: 99%

Evaluating the Phytochemical Composition and Antioxidant Activity of Leaves of Different Rose Varieties

Sharma,

Awasthi,

Kumari

2023

IJPB

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Rose is a commercially significant floricultural crop that has been used for various industrial as well as decoration purposes. Along with the beautification of rose flowers, their leaves are enriched with different biologically active compounds having various therapeutic uses. The current study was performed on the phytochemical and antioxidant activity of aqueous extracts of rose leaves. In our study, we found there are consequential variations observed in all the parameters, viz., total chlorophyll, carotenoids, total anthocyanin, total phenol, flavonoids, and antioxidant activity, among all the varieties. Among the ten varieties, Thelma Barlow revealed the maximum phenolic content (35.19 mg/g FW), flavonoids content (15.97 mg/g FW), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of IC50 value (206.86 ± 0.49 µg/mL), and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (301.62 ± 2.31 µg/mL). The variety Grand Amore presented the highest values for total chlorophyll (1.41 mg/g FW) and carotenoids (36.29 mg/g FW) content compared to other varieties. Also, a comparative correlation was studied amongst the phytochemicals such as anthocyanin content, total phenolic content, flavonoids, and antioxidant activities. Amongst the different rose varieties, Grand Amore and Thelma Barlow contain higher antioxidant potential, owing to their greater phytochemical activity. From our findings, we collectively concluded that fresh rose leaves contain potentially higher phenolic and flavonoid content, i.e., are responsible for higher antioxidant activity, which can be utilized for various pharmacological as well as food industries.

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Review on Different Methods to Assess the Antioxidant Activity of Some Common Plants of Indian Traditional Medicine

Cited by 2 publications

References 7 publications

Novel Cell-Based Assay to Investigate Monoacylglycerol Acyltransferase 2 Inhibitory Activity Using HIEC-6 Cell Line

Novel Cell-Based Assay to Investigate Monoacylglycerol Acyltransferase 2 Inhibitory Activity Using HIEC-6 Cell Line

Evaluating the Phytochemical Composition and Antioxidant Activity of Leaves of Different Rose Varieties

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