Review Paper: Burn Coverage Technologies: Current Concepts and Future Directions

Cell proliferation on three-dimensional chitosan–agarose–gelatin cryogel scaffolds for tissue engineering applications

“…For plotting the standard cell number curve cell pellet of 8 Â 10 6 was prepared and was digested overnight at 60 C in papain. Using papain as blank, serial dilutions of papain digest were prepared, e.g., 8.0 Â 10 6 , 4.0 Â 10 6 , 2.0 Â 10 6 , 0.5 Â 10 6 , 0.25 Â 10 6 and 1.25 Â 10 6 cells per ml. From each cell density 37.5 ml aliquot was transferred to the 24 well plate followed by the addition of 0.75 ml Hoechst working solution and 0.5 ml Hoechst dilution buffer to each well.…”

“…Various acellular dermal constructs like integra, alloderm, biobrane are available for the treatment of acute wounds and full thickness burn injury patients (6,7). These approaches have the associated drawbacks like they are expensive, require frequent dressing changes or make the patient prone to the subsequent secondary bacterial infections (8). So there is a demand for other alternative treatment strategies for burns or other skin injuries.…”

Porcine skin as a source of biodegradable matrices: alkaline treatment and glutaraldehyde crosslinking

“…Moreover, tissue loss can be extensive and the healing process may not be sufficient for a theraupetic healing of the tissue. Thus, the use of grafts should be considered [2,3] . Acellular matrices for tissue engineering are currently being prepared to serve as scaffolds for new tissue growth [4] .…”

Bioprinting of Skin

“…Noncellular dermal substitutes composed of various materials have been used in the clinic [17,26,27,[29][30][31][32][33]. Integra ® (Integra LifeSciences, Plainsboro, New Jersey) is an example of a noncellular dermal substitute bilayer construct containing type I bovine collagen with chondroitin-6-sulfate and a silicone membrane that can be used to create a multilayer system [27,33].…”