Review Paper on Cell Membrane Electroporation of Microalgae using Electric Field Treatment Method for Microalgae Lipid Extraction

Joannes, Costantine; Sipaut, Coswald Stephen; Dayou, Jedol; Yasir, Suhaimi Md; Mansa, Rachel Fran

doi:10.1088/1757-899x/78/1/012034

Cited by 33 publications

(17 citation statements)

References 20 publications

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“…Denaturation of proteins can occur in alkali media and degradation of pigments in acid environments (G€ unerken et al, 2015). (2015), Gonçalves et al (2013Gonçalves et al ( ), G€ unerken et al (2015, Halim et al (2012a), Joannes et al (2015), Kim et al (2013), Kumar et al (2015), Lee et al (2012), McMillan et al (2013), Mubarak et al (2015), Passos et al (2015a,b), and Show et al (2015).…”

Section: (Bio)chemical Methods For Cell Wall Disruptionmentioning

confidence: 99%

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Cell disruption technologies

Dhondt

Martín-Juárez

Bolado

et al. 2017

Microalgae-Based Biofuels and Bioproducts

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IntroductionAlgal cell walls separate the inside cell content from the environment to protect the cell against desiccation, pathogens, and predators while still allowing exchange of compounds. Toward application of algae biomass as a sustainable resource, disruption of this cell wall (¼cell disruption) is an essential pretreatment step to maximize product recovery in downstream processes of the algae biorefinery. Also for direct use of algae in feed or food, cell rupture is required to increase the bioavailability of algae constituents. Depending on the cell wall structure, the size, and the shape of algae, cell disruption can be challenging. A variety of cell disruption methods is currently available, and new approaches are being elaborated in parallel. Since downstream processing is responsible for a large part of the operational costs in the whole production chain, cell disruption technologies should be low cost and energy efficient and result preferably in high product quality. This chapter provides information on cell wall types and gives an overview of physical-mechanical and (bio-)chemical cell disruption technologies with attention to development stage, energy efficiency, product quality, costs, emerging approaches, and applicability on large scale. Cell wall types in various groups of microalgae and cyanobacteriaThe cell wall composition and architecture of algae and cyanobacteria are highly variable ranging from tiny membranes to multilayered complex structures. Despite the importance of algal cell wall properties in biotechnology, little structural information is available for most species (Scholz et al., 2014). Based on the complexity of surface structures, four cell types could be distinguished (Barsanti and Gualtieri, 2006;Lee, 2008) (Fig. 6.1). A simple cell membrane (Fig. 6.1, Type 1) is present in short-lived stages (e.g., gametes), chrysophytes, raphidophytes, green algae Dunaliella, and haptophytes Isochrysis. It consists of a lipid bilayer with integrated and peripheral proteins. Sometimes a cap of glycolipids and glycoproteins envelops the outer surface of cell membrane. Cell membranes with additional extracellular material are known in cyanobacteria and many groups of algae, including palmelloid phases. It is the most diverse cell wall type that includes various membrane-associated structures (cell wall, Microalgae-Based Biofuels and Bioproducts. http://dx

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Section: (Bio)chemical Methods For Cell Wall Disruptionmentioning

confidence: 99%

“…Pulse electric field is a promising novel technique for cell disruption (Joannes et al, 2015). The microalgal suspension is placed between two electrodes and a pulsed electric field is applied to cause electroporation to increase the permeability of the cell wall.…”

Section: Physical Methods For Cell Wall Disruptionmentioning

confidence: 99%

Cell disruption technologies

Dhondt

Martín-Juárez

Bolado

et al. 2017

Microalgae-Based Biofuels and Bioproducts

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“…In the scenarios 1, 4 and 5, the efficiency of lipids extraction (75%, 60% and 90%, respectively) was assessed. PEF extraction efficiencies between 60% and 90% have already been reported in the literature [46,49,50]. Since lipids extraction efficiency is easier to control than cell lipid content, this value was assumed to be constant (25%).…”

Section: Scenarios Descriptionmentioning

confidence: 99%

“…Regarding microalgal products, PEF is considered to have high potential for the extraction of compounds, due to the low energy consumption, easy scale-up and low operational costs. This extraction method does not use any toxic extraction solvent (not requiring a solvent recovery step) and is highly effective when directly applied to wet feedstocks [49,65,67]. PEF was already applied to extract lipids from C. vulgaris [46,68,69].…”

Section: Cell Disruption and Lipids Extractionmentioning

confidence: 99%

“…Considering the base scenario, presenting a PEF efficiency of 75% [49], total microalgal oil extracted, with a density of 0.86 kg¨L´1 [70], corresponds to 3.4 m 3¨d ay´1. For the other studied PEF efficiencies, 60% (scenario 4) and 90% (scenario 5), total lipids extracted are 2.7 and 5.1 m 3¨d ay´1, respectively.…”

Section: Cell Disruption and Lipids Extractionmentioning

confidence: 99%

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Integration of Microalgae-Based Bioenergy Production into a Petrochemical Complex: Techno-Economic Assessment

et al. 2016

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Abstract:The rapid development of modern society has resulted in an increased demand for energy, mainly from fossil fuels. The use of this source of energy has led to the accumulation of carbon dioxide (CO 2 ) in the atmosphere. In this context, microalgae culturing may be an effective solution to reduce the CO 2 concentration in the atmosphere, since these microorganisms can capture CO 2 and, simultaneously, produce bioenergy. This work consists of a techno-economic assessment of a microalgal production facility integrated in a petrochemical complex, in which established infrastructure allows efficient material and energy transport. Seven different scenarios were considered regarding photosynthetic, lipids extraction and anaerobic digestion efficiencies. This analysis has demonstrated six economically viable scenarios able to: (i) reduce CO 2 emissions from a thermoelectric power plant; (ii) treat domestic wastewaters (which were used as culture medium); and (iii) produce lipids and electrical and thermal energy. For a 100-ha facility, considering a photosynthetic efficiency of 3%, a lipids extraction efficiency of 75% and an anaerobic digestion efficiency of 45% (scenario 3), an economically viable process was obtained (net present value of 22.6 million euros), being effective in both CO 2 removal (accounting for 1.1ˆ10 4 t per year) and energy production (annual energy produced was 1.6ˆ10 7 kWh and annual lipids productivity was 1.9ˆ10 3 m 3 ).

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