Revised Manuscript Submitted to Asian Journal of Biotechnology and Bioresource Technology Enhancement of Triterpenoids formation of Antrodia cinnamomea in thin Stillage-submerged Culture by Shifting the Shaking Rate and Adding Soybean Oil

Abstract

Antrodia cinnamomea is an endemic medical mushroom in Taiwan. It is well known that the major effective components in this medical fungus are polysaccharides, triterpenoids, and steroids; it is found only in Taiwan. Its anti-tumour activity remains Current research interest is focused on its anti-tumour activity from secondary metabolites of triterpenoids. However, the main disadvantage of mycelia produced by liquid cultures is the quite small amount of triterpenoid. In this study, various cultivation strategies were tested to investigate their effects on the production of triterpenoids in thin stillage submerged cultures of A. cinnamomea. The results indicate that when thin stillage was reused in shaking flask culture at a constant speed, crude triterpenoids production reached the level of 43.04 mg/L at a low inoculum size of 1% at d-20, which was twice that of the control of high inoculum size of 10%. This study demonstrates that diminishing inoculum size has a