Revisiting dextran effect on red blood cell to understand the importance of rouleaux distribution and red blood cell-endothelial cell adhesion

Mahalakshmi, Vijayaraghavan; Chatterjee, Suvro; Sumantran, Venil N.; Jayavelu, Tamilselvan

doi:10.1007/s13399-022-02851-0

Cited by 2 publications

(3 citation statements)

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“…Another study of RBC aggregation stability asses the the distribution of RBC rouleaux shape and its adhesive nature towards endothelial cell in a non-flowing environment with biopolymer dextran present (known to be the RBC aggregation promoter). Authors observed constant rouleaux I-, L-, Y-shapes and clump-shaped with gradient change in dextran concentration (450-650 kDa) as well as dextran (7.5% and 10%) consistently promoting single RBC adhesion towards endothelial cell then aggregated RBCs [121]. Finally, the RBC aggregation has also been studied by using a microfluidic biosensor with continuously varying blood flows to determine the aggregation index vs shear rate dependency [112], and ultrasound-based methods for quantifying RBC aggregation [122].…”

Section: Rbc Flow Analysis In Bifurcating Channelsmentioning

confidence: 98%

“…Biosensors 2021, 11, x FOR PEER REVIEW 11 of 51 (450-650 kDa) as well as dextran (7.5% and 10%) consistently promoting single RBC adhesion towards endothelial cell then aggregated RBCs [121]. Finally, the RBC aggregation has also been studied by using a microfluidic biosensor with continuously varying blood flows to determine the aggregation index vs shear rate dependency [112], and ultrasoundbased methods for quantifying RBC aggregation [122].…”

Section: Rbc Agglutination/aggregation In Microfluidic Environmentmentioning

confidence: 99%

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Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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The utilizations of microfluidic chips for single RBC (red blood cell) studies have attracted great interests in recent years to filter, trap, analyze, and release single erythrocytes for various applications. Researchers in this field have highlighted the vast potential in developing micro devices for industrial and academia usages, including lab-on-a-chip and organ-on-a-chip systems. This article critically reviews the current state-of-the-art and recent advances of microfluidics for single RBC analyses, including integrated sensors and microfluidic platforms for microscopic/tomographic/spectroscopic single RBC analyses, trapping arrays (including bifurcating channels), dielectrophoretic and agglutination/aggregation studies, as well as clinical implications covering cancer, sepsis, prenatal, and Sickle Cell diseases. Microfluidics based RBC microarrays, sorting/counting and trapping techniques (including acoustic, dielectrophoretic, hydrodynamic, magnetic, and optical techniques) are also reviewed. Lastly, organs on chips, multi-organ chips, and drug discovery involving single RBC are described. The limitations and drawbacks of each technology are addressed and future prospects are discussed.

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Section: Rbc Flow Analysis In Bifurcating Channelsmentioning

confidence: 98%

Section: Rbc Agglutination/aggregation In Microfluidic Environmentmentioning

confidence: 99%

Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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“…Dextran is often used to study the behavior of erythrocytes due to its ability to induce their aggregation. Depending on its molecular weight and concentration, it can simulate certain physiological or pathological conditions [25,26].…”

Section: Introductionmentioning

confidence: 99%

The Impact of COVID-19 on Cellular Factors Influencing Red Blood Cell Aggregation Examined in Dextran: Possible Causes and Consequences

Bosek,

Wybranowski,

Napiórkowska-Mastalerz

et al. 2023

IJMS

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Several studies have indicated that COVID-19 can lead to alterations in blood rheology, including an increase in red blood cell aggregation. The precise mechanisms behind this phenomenon are not yet fully comprehended. The latest findings suggest that erythrocyte aggregation significantly influences microcirculation, causes the formation of blood clots in blood vessels, and even damages the endothelial glycocalyx, leading to endothelial dysfunction. The focus of this research lies in investigating the cellular factors influencing these changes in aggregation and discussing potential causes and implications in the context of COVID-19 pathophysiology. For this purpose, the aggregation of erythrocytes in a group of 52 patients with COVID-19 pneumonia was examined in a 70 kDa Dextran solution, which eliminates the influence of plasma factors. Using image analysis, the velocities and sizes of the formed aggregates were investigated, determining their porosity. This study showed that the process of erythrocyte aggregation in COVID-19 patients, independent of plasma factors, leads to the formation of more compact, denser, three-dimensional aggregates. These aggregates may be less likely to disperse under circulatory shear stress, increasing the risk of thrombotic events. This study also suggests that cellular aggregation factors can be responsible for the thrombotic disorders observed long after infection, even when plasma factors have normalized. The results and subsequent broad discussion presented in this study can contribute to a better understanding of the potential complications associated with increased erythrocyte aggregation.

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Revisiting dextran effect on red blood cell to understand the importance of rouleaux distribution and red blood cell-endothelial cell adhesion

Cited by 2 publications

References 63 publications

Advances in Microfluidics for Single Red Blood Cell Analysis

Advances in Microfluidics for Single Red Blood Cell Analysis

The Impact of COVID-19 on Cellular Factors Influencing Red Blood Cell Aggregation Examined in Dextran: Possible Causes and Consequences

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