RFLP diversity of common bean (Phaseolus vulgaris) in its centres of origin

Abstract

Eighty-five wild and cultivated accessions of common bean (Phaseolus vulgaris L.), representing a wide geographic area in the centres of domestication were tested for restriction fragment length polymorphisms (RFLPs). Genomic DNA was digested with one of three restriction enzymes (EcoRI, EcoRV, and HindIII) and hybridized to 12 probes distributed throughout the common bean genome. Accessions could be classified into two major groups with a distinct geographical distribution in Middle America and the Andes. Within each gene pool, cultivated accessions clustered together with wild forms from the same geographical area supporting the multiple domestications hypothesis for this crop. Estimates of Nei's genetic distances among the cultivated races from the two different gene pools varied from 0.12 to 0.56 and among races from the same gene pool from 0.04 to 0.12, suggesting that the divergence in Phaseolus vulgaris has reached the subspecies level. The level of genetic diversity (Ht = 0.38) was twice the value obtained with isozyme analysis. Genetic diversity within races (Hs = 0.27) was four to five times higher compared with isozymes, but genetic diversity between races (Dst = 0.11) was similar for both categories of markers. These results corroborate previous studies on the characterization of genetic diversity in common bean that clearly showed two distinct gene pools, Middle American and Andean. Moreover, RFLP markers are superior to isozymes because they provide better coverage of the genome and reveal higher level of polymorphisms.

Investigation of the domestication of common bean (Phaseolus vulgaris) using multilocus sequence data

“…This observation is consistent with earlier research (Becerra Velasquez and Gepts 1994;Singh et al 1991b;Freyre et al 1996;McClean et al 2004;McClean and Lee 2007;). For the landraces we analysed, the nucleotide diversity of MA was higher than that the Andean gene pool, similar to earlier studies (Cattan-Toupance et al 1998;Beebe et al 2001;McClean et al 2004;McClean and Lee 2007;Rossi et al 2009).…”

“…Two large gene pools of wild types were identified based on phaseolin seed protein variation Gepts 1990), DNA marker diversity (Becerra Velasquez and Gepts 1994;Sonnante et al 1994;Freyre et al 1996;Tohme et al 1996), morphology (Evans 1976;Gepts and Debouck 1991), isozymes (Koenig and Gepts 1989), mitochondrial DNA variation (Khairallah et al 1992) and amplified fragment length polymorphism (AFLP) (Rossi et al 2009) and short sequence repeats (SSR) ) marker data.…”

“…The presence of distinct groups of landraces has been described at both the morphological (Singh et al 1991a) and molecular level Singh et al 1991b;Becerra Velasquez and Gepts 1994;Freyre et al 1996). Singh et al (1991a) classified the MA cultivars into three races -lowland race Mesoamerican (M) and highland races Durango and Jalisco.…”

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

“…Since, the cultivar IC 251431 and IC 251434 occupy a distinct place in the dendrogram. The level of polymorphism observed in the entire data set was found to be 90.90%, which were in more or less harmony with those of Velasquez and Gepts (1994), who observed 70% polymorphism in Phaseolus vulgaris. The results obtained further strengthened the previous findings of Nodari et al (1992) and Silberstein et al (1999).…”

Cacao domestication I: the origin of the cacao cultivated by the Mayas

“…Furthermore, Cuatrecasas (1964) hypothesized that wild plants from the Lacandona rainforest from Mexico were possible ancestors of domesticated cacao. Separate simultaneous origin and domestication in both Central and South America has been suggested for the common bean, Phaseolus vulgaris (Velasquez and Gepts, 1994).…”