Rhinovirus/enterovirus RNA in tonsillar tissue of children with tonsillar disease

Comparison of Results of Detection of Rhinovirus by PCR and Viral Culture in Human Nasal Wash Specimens from Subjects with and without Clinical Symptoms of Respiratory Illness

“…The forward primer sequence corresponded to nucleotides 414 to 432 of HRV-89, as previously described (11). The reverse primer sequence corresponded to the reverse complement of nucleotides 461 to 481 of HRV-89 (25). The double-labeled fluorescent probe sequence corresponded to nucleotides 438 to 459 of HRV-89.…”

“…Recent reports suggest that PCR detection extends the scope of HRV illness to include lower respiratory tract illness (10) and establishes a strong association between rhinovirus and exacerbations of asthma (12,26). HRV has been shown to replicate in cells of both the upper (14, 27) and lower (21) respiratory tracts and can grow at the higher temperature of the lower respiratory tract (22).However, more limited studies have detected HRV by PCR in 12 to 20% of asymptomatic children and in a relatively high percentage of adenoid and tonsillar tissues, suggesting that identification of HRV by PCR may not necessarily confirm it as the etiologic agent (16,20,23,25). Identification of rhinovirus infection by PCR may not be accompanied by a serologic response, an unusual occurrence with most symptomatic respiratory viral infections (4).…”

“…However, more limited studies have detected HRV by PCR in 12 to 20% of asymptomatic children and in a relatively high percentage of adenoid and tonsillar tissues, suggesting that identification of HRV by PCR may not necessarily confirm it as the etiologic agent (16,20,23,25). Identification of rhinovirus infection by PCR may not be accompanied by a serologic response, an unusual occurrence with most symptomatic respiratory viral infections (4).…”

Decreasing incidence of adenotonsillar problems in Dutch general practice: real or artefact?

“…Bacterial species that have been isolated are: Haemophilus influenzae, Streptococcus pyogenes, Streptococcus pneumonia, and Staphylococcus aureus. [18][19][20][21][22][23][24][25][26][27][28][29][30] A decrease in the incidence of these pathogens could explain a decrease in adenotonsillar problems. The present study uses a national general practice database to explore the trend in the incidence of hypertrophy and recurrent infections of the tonsils/adenoids, and the causes of any changes identified, among children aged 0-14 years in the Netherlands.…”

“…• based on the literature, the major microbial pathogens of hypertrophy and infections of the tonsils/adenoids were listed; [18][19][20][21][22][23][24][25][26][27][28][29][30] • next, other frequent clinical manifestations of these pathogens were looked for and the corresponding ICPC codes selected. Clinical manifestations involving a general ICPC code, such as coughing (R05), were excluded; and • for each pathogen a cluster of ICPC codes was composed (Appendix 1).…”