Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response

Jayapala, Naveen; Mallikarjunaiah, Navya Hulikunte; Hariprasad, P.; Hithamani, Gavirangappa; Ramachandrappa, Niranjana Siddapura

doi:10.1186/s41938-019-0148-2

Cited by 57 publications

(38 citation statements)

References 32 publications

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“…The antagonistic Bacillus sp. suppressed anthracnose disease of chili by the activation of defense-related enzymes and the accumulation of phenolic compounds [ 86 ]. Similarly, Bacillus sp.…”

Section: Systemically Induced Disease Resistancementioning

confidence: 99%

The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

2020

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Bacillus spp. produce a variety of compounds involved in the biocontrol of plant pathogens and promotion of plant growth, which makes them potential candidates for most agricultural and biotechnological applications. Bacilli exhibit antagonistic activity by excreting extracellular metabolites such as antibiotics, cell wall hydrolases, and siderophores. Additionally, Bacillus spp. improve plant response to pathogen attack by triggering induced systemic resistance (ISR). Besides being the most promising biocontrol agents, Bacillus spp. promote plant growth via nitrogen fixation, phosphate solubilization, and phytohormone production. Antagonistic and plant growth-promoting strains of Bacillus spp. might be useful in formulating new preparations. Numerous studies of a wide range of plant species revealed a steady increase in the number of Bacillus spp. identified as potential biocontrol agents and plant growth promoters. Among different mechanisms of action, it remains unclear which individual or combined traits could be used as predictors in the selection of the best strains for crop productivity improvement. Due to numerous factors that influence the successful application of Bacillus spp., it is necessary to understand how different strains function in biological control and plant growth promotion, and distinctly define the factors that contribute to their more efficient use in the field.

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Section: Systemically Induced Disease Resistancementioning

confidence: 99%

The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

2020

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“…It was previously reported that the induction of plant defense related enzymes is related to the plant defense system and induced resistance by PGPR inoculation and colonization ( Liang et al, 2011 ). The accumulation of defense related enzymes after PGPR application has also been reported in cucumber ( Liang et al, 2011 ), chili ( Jayapala et al, 2019 ), and tomato ( Ramamoorthy et al, 2002b ). Dukare and Paul (2021) also found that rhizobacterial inoculation conferred resistance to pigeon pea seedlings by inducing and improving the production of defense related enzymes and phenolics.…”

Section: Discussionmentioning

confidence: 76%

“…Capsaicinoid compound derived from chili has many ethnopharmacological applications including anticancer, anti-obesity treatment, temperature regulation, pain therapy, and antioxidant effects ( Meghvansi et al, 2010 ). Chili crop is vulnerable to more than 100 different types of pathogens during its various growth stages ( Jayapala et al, 2019 ). Of the different microbial diseases that affect this plant, the damping-off and root rot disease caused by Pythium myriotylum Drechsler.…”

Section: Introductionmentioning

confidence: 99%

Biological Control of Chili Damping-Off Disease, Caused by Pythium myriotylum

Hyder¹,

Gondal

Rizvi³

et al. 2021

Front. Microbiol.

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Pythium myriotylum is a notorious soil-borne oomycete that causes post-emergence damping-off in chili pepper. Of various disease management strategies, utilization of plant growth promoting rhizobacteria (PGPR) in disease suppression and plant growth promotion is an interesting strategy. The present study was performed to isolate and characterize PGPR indigenous to the chili rhizosphere in Pakistan, and to test the potential to suppress the damping-off and plant growth promotion in chili. Out of a total of 28 antagonists, eight bacterial isolates (4a2, JHL-8, JHL-12, 1C2, RH-24, 1D, 5C, and RH-87) significantly suppressed the colony growth of P. myriotylum in a dual culture experiment. All the tested bacterial isolates were characterized for biochemical attributes, and 16S rRNA sequence based phylogenetic analysis identified these isolates as Flavobacterium spp., Bacillus megaterium, Pseudomonas putida, Bacillus cereus, and Pseudomonas libanensis. All the tested bacterial isolates showed positive test results for ammonia production, starch hydrolase (except 4a2), and hydrogen cyanide production (except 4a2 and 1D). All the tested antagonists produced indole-3-acetic acid (13.4–39.0 μg mL–1), solubilized inorganic phosphate (75–103 μg mL–1), and produced siderophores (17.1–23.7%) in vitro. All the tested bacterial isolates showed varying levels of susceptibility and resistance response against different antibiotics and all these bacterial isolates were found to be non-pathogenic to chili seeds and notably enhanced percentage seed germination, plumule, redical length, and vigor index over un-inoculated control. Additionally, under pathogen pressure, bacterization increased the defense related enzymes such as Peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) activates. Moreover, the treatment of chili seeds with these bacterial isolates significantly suppressed the damping-off caused by P. myriotylum and improved PGP traits compared to the control. In addition, a positive correlation was noticed between shoot, root length, and dry shoot and root weight, and there was a negative correlation between dry shoot, root weight, and seedling percentage mortality. These results showed that native PGPR possesses multiple traits beneficial to the chili plants and can be used to develop eco-friendly and effective seed treatment formulation as an alternative to synthetic chemical fungicides.

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“…It was previously reported that the induction of plant defense related enzymes is related with the plant defense system and induced resistance by PGPR inoculation and colonization (Liang et al, 2011). Accumulation of defense related enzymes after PGPR application has been reported in cucumber (Liang et al, 2011), chilli (Jayapala et al, 2019) and tomato (Ramamoorthy et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Capsaicinoid compound derived from chilli has many ethnopharmacological applications including anticancer, anti-obesity treatment, temperature regulation, pain therapy, and as antioxidant (Meghvansi et al, 2010). Chili crop is vulnerable to more than 100 different types of pathogens during various growth stages (Jayapala et al, 2019). Of different microbial diseases, damping off and root rot disease caused by Pythium myriotylum Drechsler., is the most devastating disease in terms of seedling mortality at very early growth stages in nurseries when the seedlings are in cotyledonous stage.…”

Section: Introductionmentioning

confidence: 99%

Biological control of chili damping off disease, caused byPythium myriotylum

Hyder

Gondal

Rizvi³

et al. 2020

Preprint

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Pythium myriotylum is a notorious soil-borne oomycete causes post-emergence damping off in chilli pepper. Of various disease management strategies, utilization of plant growth promoting rhizobacteria (PGPR) in disease suppression and plant growth promotion is eye catching strategy. The present study was performed to isolate and characterize PGPR indigenous to chili rhizosphere in Pakistan, and to test their potential to suppress damping off and plant growth promotion in chilli. Out of total 28 antagonists, 8 bacterial isolates (4a2, JHL-8, JHL-12, 1C2, RH-24, 1D, 5C and RH-87) significantly suppressed the colony growth of P. myriotylum in dual culture experiment. All the tested bacterial isolates were characterized for biochemical attributes, and 16S rRNA sequence based phylogenetic analysis identified these isolates as Flavobacterium spp., Bacillus megaterium, Pseudomonas putida, Bacillus cereus and Pseudomonas libanensis. All the tested bacterial isolates showed positive test results for ammonia production, starch hydrolase (except 4a2), and hydrogen cyanide production (except 4a2 and 1D). All the tested antagonists produced indole-3-acetic acid (13.4-39.0 μg ml−1), solubilized inorganic phosphate (75–103 μgml-1) and produced siderophores (17.1–23.7%) in vitro. All the tested bacterial isolates showed varied level of susceptibility and resistance response against different antibiotics and all these bacterial isolates were found non-pathogenic to chill seeds and notably enhanced percentage seed germination, plumule, redical length and vigor index over un-inoculated control. Additionally, under pathogen pressure, bacterization increased the defense related enzymes (PO, PPO and PAL) activates. Moreover, chilli seeds treatment with these bacterial isolates significantly suppressed damping-off caused by P. myriotylum, and improved PGP traits as compared to control. In addition, a positive correlation was noticed between shoot, root length and dry shoot and root weigh and a negative correlation was seen between dry shoot, root weight and seedling percentage mortality. These results showed that native PGPR possess multiple traits beneficial to the chilli plants and can be used to develop eco-friendly and effective seed treatment formulation as an alternative to synthetic chemical fungicides.

show abstract

Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response

Cited by 57 publications

References 32 publications

The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

Biological Control of Chili Damping-Off Disease, Caused by Pythium myriotylum

Biological control of chili damping off disease, caused byPythium myriotylum

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