Rhizobacterial Community Structures Associated with Native Plants Grown in Chilean Extreme Environments

Jorquera, Milko A.; Maruyama, Fumito; Ogram, Andrew; Navarrete, Oscar; Lagos, Lorena M.; Inostroza, Nitza G.; Acuña, Jacquelinne J.; Rilling, Joaquín I.; Mora, Marı́a de la Luz

doi:10.1007/s00248-016-0813-x

Cited by 55 publications

(48 citation statements)

References 58 publications

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“…This was revealed by the abundance of Rhizobium in the rhizosphere of soil of inoculated seedlings. This finding supports the well‐known role of rhizobia on the growth of native legumes of arid lands (Jorquera et al, ) and the documented interactions of rhizobia with many species of PGPB in agriculture (Martínez‐Hidalgo & Hirsch, ). Inoculation with B. pumilus ES4 also seems to have slightly reduced the abundance of several members of its own phylum (Firmicutes).…”

Section: Discussionsupporting

confidence: 87%

Root growth improvement of mesquite seedlings and bacterial rhizosphere and soil community changes are induced by inoculation with plant growth‐promoting bacteria and promote restoration of eroded desert soil

Galaviz¹,

Lopez²,

de‐Bashan

et al. 2018

Land Degrad Dev

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Soil degradation is an ecological disturbance, usually human‐caused, that negatively affects the vegetation and climate of an ecosystem, particularly arid and semiarid environments. These degraded soils can be restored by using native perennial plants inoculated with specific microorganisms. We studied the changes in root growth and the rhizosphere bacterial community of mesquite seedlings (Prosopis articulata) after inoculation with the endophytic bacteria Bacillus pumilus ES4, over 3 cycles of growth in the same soil under desert climatic conditions, and found that inoculation significantly enhanced root biomass during the growth cycles but not shoot biomass or root and shoot lengths. Fluorescent in situ hybridization analysis demonstrated that B. pumilus colonized the root cap, apical meristem, and elongation zone, forming small colonies, on roots from soil‐grown mesquite. Inoculation also significantly changed the bacterial community structure of rhizophere and nonrhizosphere (without plants) soils based on denaturing gradient gel electrophoresis profiles. The changes were highly stable, and the bacterial community structure was maintained throughout the experimental period and not affected by plant replacement. The 16S rRNA pyrosequencing confirmed the changes on structure of bacterial community and revealed an impact on the top taxonomic levels analyzed. The rhizospheres of inoculated plants showed a significant increase in the abundance of Proteobacteria and Acidobacteria coupled with a concomitant decrease in Actinobacteria, whereas an opposite response was observed in nonrhizospheric degraded soils. Overall, inoculation with B. pumilus reduced bacterial diversity but increased the Rhizobium population in the soil. The class Bacilli, despite B. pumilus inoculum, showed minimal variation.

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Section: Discussionsupporting

confidence: 87%

Root growth improvement of mesquite seedlings and bacterial rhizosphere and soil community changes are induced by inoculation with plant growth‐promoting bacteria and promote restoration of eroded desert soil

Galaviz¹,

Lopez²,

de‐Bashan

et al. 2018

Land Degrad Dev

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“…and Stipa sp. also native of the Atacama Desert [72] and in other arid ecosystems, Tribulus terrestris, Zygophyllum simplex, Panicum turgidum, and Euphorbia granulata of Saudi Arabia Desert [73]. Also, recent studies of endophytic bacterial communities in Distichilis spicata and Pluschea absinthioides have identified Proteobacteria as a higher dominant phylum [74].…”

Section: Discussionmentioning

confidence: 99%

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

et al. 2020

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The rhizosphere microbiome is key in survival, development, and stress tolerance in plants. Salinity, drought, and extreme temperatures are frequent events in the Atacama Desert, considered the driest in the world. However, little information of the rhizosphere microbiome and its possible contribution to the adaptation and tolerance of plants that inhabit the desert is available. We used a high-throughput Illumina MiSeq sequencing approach to explore the composition, diversity, and functions of fungal and bacterial communities of the rhizosphere of Baccharis scandens and Solanum chilense native plants from the Atacama Desert. Our results showed that the fungal phyla Ascomycota and Basidiomycota and the bacterial phyla Actinobacteria and Proteobacteria were the dominant taxa in the rhizosphere of both plants. The linear discriminant analysis (LDA) effect size (LefSe) of the rhizosphere communities associated with B. scandens showed the genera Penicillium and Arthrobacter were the preferential taxa, whereas the genera Oidiodendron and Nitrospirae was the preferential taxa in S. chilense. Both plant showed similar diversity, richness, and abundance according to Shannon index, observed OTUs, and evenness. Our results indicate that there are no significant differences (p = 0.1) between the fungal and bacterial communities of both plants, however through LefSe, we find taxa associated with each plant species and the PCoA shows a separation between the samples of each species. This study provides knowledge to relate the assembly of the microbiome to the adaptability to drought stress in desert plants.

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“…The quality and quantity of DNA extracts were measured using the Plate reader Infinite 200 PRO (TECAN, Switzerland). DNA purity was assessed by the determination of the A260/A280 absorbance ratios, and only DNA extracts with absorbance ratios of 1.8-2.0 were used for bacterial community analyses [37].…”

Section: Dna Extraction Polymerase Chain Reaction and Denaturing Grmentioning

confidence: 99%

Effects of Heavy Metals on Phyllosphere and Rhizosphere Microbial Community of Bothriochloa ischaemum

et al. 2018

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Copper mining has resulted in severe damage to the ecological environment of mining areas. This study investigated heavy metal distribution in plants and compared the driving factors between aboveground and subsurface microorganisms, as well as the diversity in rhizosphere and non-rhizosphere soil microbial community response to heavy metal transfer factors in a copper tailings dam. We analyzed phyllosphere and soil microbial community using high-throughput sequencing and denaturing gradient gel electrophoresis, respectively. Although we detected chromium in aboveground and subsurface of Bothriochloa ischaemum specimens, no chromium was detected in soil. Total nitrogen was negatively correlated to the carbon and nitrogen ratios of plants and soil, respectively, while the total sulfur was negatively correlated to cadmium in roots. On the contrary, soil sulfur was positively correlated to cadmium in soil. Moreover, soil sulphur was the main influencing factor on the soil bacterial community, while ammonium nitrogen, total nitrogen, and zinc were the driving factors of fungi diversity in non-rhizosphere soil. Fungi diversity in the rhizosphere was significantly correlated to phosphatase, and fungi diversity in the non-rhizosphere was significantly correlated to sucrose enzymes. The transfer factor of lead was negatively correlated to rhizosphere fungi diversity, and the transfer factor of copper was significantly correlated to non-rhizosphere bacterial diversity. Results from this study may offer some scientific reference for the improvement of plant-microbe remediation efficiency. At the same time, this study could provide an ecological basis for further studies on soil ecosystem restoration and degradation mechanisms that are associated with copper tailings dams.

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Rhizobacterial Community Structures Associated with Native Plants Grown in Chilean Extreme Environments

Cited by 55 publications

References 58 publications

Root growth improvement of mesquite seedlings and bacterial rhizosphere and soil community changes are induced by inoculation with plant growth‐promoting bacteria and promote restoration of eroded desert soil

Root growth improvement of mesquite seedlings and bacterial rhizosphere and soil community changes are induced by inoculation with plant growth‐promoting bacteria and promote restoration of eroded desert soil

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

Effects of Heavy Metals on Phyllosphere and Rhizosphere Microbial Community of Bothriochloa ischaemum

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