Rhizobium leguminosarum bv. trifolii NodD2 Enhances Competitive Nodule Colonization in the Clover-Rhizobium Symbiosis

Ferguson, Shaun; Major, Anthony S.; Sullivan, John T.; Bourke, Scott D.; Kelly, Simon; Perry, Benjamin J.; Ronson, Clive W.

doi:10.1128/aem.01268-20

Cited by 17 publications

(9 citation statements)

References 85 publications

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“…viciae strains able to use homoserine as a carbon and nitrogen source were found to be prevalent in pea nodules (Hynes and O'Connell, 1990;Vanderlinde et al, 2014). However, this does not exclude the possibility that some genes present on the chromosome may also contribute to the symbiotic phenotype and its variation (Boivin et al, 2020;Ferguson et al, 2020).…”

Section: Genetic Stability Of Inoculant Strainsmentioning

confidence: 99%

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Competition, Nodule Occupancy, and Persistence of Inoculant Strains: Key Factors in the Rhizobium-Legume Symbioses

et al. 2021

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Biological nitrogen fixation by Rhizobium-legume symbioses represents an environmentally friendly and inexpensive alternative to the use of chemical nitrogen fertilizers in legume crops. Rhizobial inoculants, applied frequently as biofertilizers, play an important role in sustainable agriculture. However, inoculants often fail to compete for nodule occupancy against native rhizobia with inferior nitrogen-fixing abilities, resulting in low yields. Strains with excellent performance under controlled conditions are typically selected as inoculants, but the rates of nodule occupancy compared to native strains are rarely investigated. Lack of persistence in the field after agricultural cycles, usually due to the transfer of symbiotic genes from the inoculant strain to naturalized populations, also limits the suitability of commercial inoculants. When rhizobial inoculants are based on native strains with a high nitrogen fixation ability, they often have superior performance in the field due to their genetic adaptations to the local environment. Therefore, knowledge from laboratory studies assessing competition and understanding how diverse strains of rhizobia behave, together with assays done under field conditions, may allow us to exploit the effectiveness of native populations selected as elite strains and to breed specific host cultivar-rhizobial strain combinations. Here, we review current knowledge at the molecular level on competition for nodulation and the advances in molecular tools for assessing competitiveness. We then describe ongoing approaches for inoculant development based on native strains and emphasize future perspectives and applications using a multidisciplinary approach to ensure optimal performance of both symbiotic partners.

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Section: Genetic Stability Of Inoculant Strainsmentioning

confidence: 99%

“…In R. leguminosarum bv. trifolii , a second copy, NodD2, enhanced nodule colonization and competitiveness in symbiosis with clover ( Ferguson et al, 2020 ). These accessory nod genes may reflect the variations within the interactions among rhizobial and plant species.…”

Section: Types Of Competition and Genetic Features Influencing Competitivenessmentioning

confidence: 99%

Competition, Nodule Occupancy, and Persistence of Inoculant Strains: Key Factors in the Rhizobium-Legume Symbioses

et al. 2021

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“…nodAseq7 and nodDseq9 occurred only as secondary sequences in this partial nod region and were designated as nodAa and nodDa, respectively ( Figure 2C-D). A third type of nodD (nodD2) was observed in some genomes flanked by transposases and no other nod genes (Kelly et al, 2018;Ferguson et al, 2020). Three nodD amplicons belong to this group.…”

Section: Geographically Distinct Sites Display a Site-specific Set Ofmentioning

confidence: 98%

Amplicons and isolates:Rhizobiumdiversity in fields under conventional and organic management

Moeskjær

Tausen

Andersen

et al. 2020

Preprint

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Background: The influence of farming on plant, animal and microbial biodiversity has been carefully studied and much debated. Here, we compare an isolate-based study of 196 Rhizobium strains to amplicon-based MAUI-seq analysis of rhizobia from 17,000 white clover root nodules. We use these data to investigate the influence of soil properties, geographic distance, and field management on Rhizobium nodule populations. Results: Overall, there was good agreement between the two approaches and the precise allele frequency estimates from the large-scale MAUI-seq amplicon data allowed detailed comparisons of rhizobium populations between individual plots and fields. A few specific chromosomal core-gene alleles were significantly correlated with soil clay content, and core-gene allele profiles became increasingly distinct with geographic distance. Field management was associated with striking differences in Rhizobium diversity, where organic fields showed significantly higher diversity levels than conventionally managed trials. Conclusions: Our results indicate that MAUI-seq is suitable and robust for assessing nodule Rhizobium diversity. We further observe possible profound effects of field management on microbial diversity, which could impact plant health and productivity and warrant further investigation.

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“…Often, rhizobia contain multiple nodD copies that encode different NodD isoforms (Ferguson et al ., 2020). These isoforms may perform divergent roles, may be involved in distinct stages during symbiotic infection, or may be required under different environmental conditions, and have been described to enhance nodulation competitiveness or to extend symbiotic host range through perception of different plant signalling molecules (Demont et al ., 1994; del Cerro et al ., 2015, 2017, 2020; Kelly et al ., 2018; Acosta‐Jurado et al ., 2019; Ferguson et al ., 2020). Once activated, NodD induces the expression of bacterial genes involved in the synthesis of the so‐called Nod factors (Broughton et al ., 2000).…”

Section: Mechanisms Behind the Limitations Of The Nodulation Process And Bacterial Tools To Overcome Themmentioning

confidence: 99%

Legume–rhizobium dance: an agricultural tool that could be improved?

Basile

Lepek

2021

Microbial Biotechnology

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Summary The specific interaction between rhizobia and legume roots leads to the development of a highly regulated process called nodulation, by which the atmospheric nitrogen is converted into an assimilable plant nutrient. This capacity is the basis for the use of bacterial inoculants for field crop cultivation. Legume plants have acquired tools that allow the entry of compatible bacteria. Likewise, plants can impose sanctions against the maintenance of nodules occupied by rhizobia with low nitrogen‐fixing capacity. At the same time, bacteria must overcome different obstacles posed first by the environment and then by the legume. The present review describes the mechanisms involved in the regulation of the entire legume–rhizobium symbiotic process and the strategies and tools of bacteria for reaching the nitrogen‐fixing state inside the nodule. Also, we revised different approaches to improve the nodulation process for a better crop yield.

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Rhizobium leguminosarum bv. trifolii NodD2 Enhances Competitive Nodule Colonization in the Clover-Rhizobium Symbiosis

Cited by 17 publications

References 85 publications

Competition, Nodule Occupancy, and Persistence of Inoculant Strains: Key Factors in the Rhizobium-Legume Symbioses

Competition, Nodule Occupancy, and Persistence of Inoculant Strains: Key Factors in the Rhizobium-Legume Symbioses

Amplicons and isolates:Rhizobiumdiversity in fields under conventional and organic management

Legume–rhizobium dance: an agricultural tool that could be improved?

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