Rhizoferrin: A complexone type siderophore of the mocorales and entomophthorales (Zygomycetes)

Thieken, Andrea; Winkelmann, G.

doi:10.1016/0378-1097(92)90579-d

Cited by 59 publications

(53 citation statements)

References 4 publications

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“…We were able to partially purify the siderophore using a series of chromatographic steps similar to those used for purification of the polycarboxylate siderophore rhizoferrin from Rhizopus spp. and other fungal species (12,40). The siderophore was retained on an anion-exchange column AG1-X8 (formate form) and could be eluted using formic acid or ammonium formate (Fig.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the Siderophore ofFrancisella tularensisand Role offslAin Siderophore Production

Sullivan¹,

Jeffery²,

Shannon

et al. 2006

J Bacteriol

210

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We determined that LVS and Schu S4 strains of the human pathogen Francisella tularensis express a siderophore when grown under iron-limiting conditions. We purified this siderophore by conventional column chromatography and high-pressure liquid chromatography and used mass spectrometric analysis to demonstrate that it is structurally similar to the polycarboxylate siderophore rhizoferrin. The siderophore promoted the growth of LVS and Schu S4 strains in iron-limiting media. We identified a potential siderophore biosynthetic gene cluster encoded by fslABCD in the F. tularensis genome. The first gene in the cluster, fslA, encodes a member of the superfamily of nonribosomal peptide synthetase-independent siderophore synthetases (NIS synthetases) characterized by the aerobactin synthetases IucA and IucC. We determined that fslA is transcribed as part of an operon with downstream gene fslB and that the expression of the locus is induced by iron starvation. A targeted in-frame nonpolar deletion of fslA in LVS resulted in the loss of siderophore expression and in a reduced ability of F. tularensis to grow under conditions of iron limitation. Siderophore activity and the ability to grow under iron limitation could be regained by introducing the fslA ؉ gene on a complementing plasmid. Our results suggest that the fslA-dependent siderophore is important for survival of F. tularensis in an iron-deficient environment.

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Section: Resultsmentioning

confidence: 99%

Characterization of the Siderophore ofFrancisella tularensisand Role offslAin Siderophore Production

Sullivan¹,

Jeffery²,

Shannon

et al. 2006

J Bacteriol

210

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“…The siderophores have shown considerable structural diversity, being classified mainly into hydroxamate and phenol-catecholate types according to their chelating groups (37). Recent discoveries of more hydrophilic siderophores have established a separate type, polyhydroxycarboxylate (complexone) (59). This type of siderophores has been reported to include rhizobactin (51), rhizoferrin (59), staphyloferrin B (13), vibrioferrin (71), and achromobactin (36).…”

mentioning

confidence: 99%

“…Recent discoveries of more hydrophilic siderophores have established a separate type, polyhydroxycarboxylate (complexone) (59). This type of siderophores has been reported to include rhizobactin (51), rhizoferrin (59), staphyloferrin B (13), vibrioferrin (71), and achromobactin (36). In gram-negative bacteria, all siderophore-mediated iron acquisition systems so far described strictly require specific outer membrane receptors as well as ATP-binding cassette (ABC) transport systems (4).…”

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confidence: 99%

Identification and Characterization of Genes Required for Biosynthesis and Transport of the Siderophore Vibrioferrin in Vibrio parahaemolyticus

et al. 2003

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In response to low iron availability, Vibrio parahaemolyticus synthesizes and secretes a polyhydroxycarboxylate-type siderophore vibrioferrin which is composed of 1 mol each of 2-ketoglutaric acid, L-alanine, ethanolamine, and citric acid. We have previously reported the cloning and characterization of the pvuA gene, which encodes the 78-kDa outer membrane receptor protein for ferric vibrioferrin. In this study, nine genes involved in the biosynthesis and transport of vibrioferrin have been identified in the genomic regions surrounding the pvuA gene. The genes were sequenced, and gene disruptants were constructed by insertion mutation for phenotype analysis. Five of the genes, named pvsABCDE, constitute an operon that is expressed under iron-limiting conditions. Homology searches of their predicted protein products suggested that the four genes pvsABDE are implicated in the biosynthesis of the siderophore. Another gene in the same operon, pvsC, encodes a putative exporter that is homologous to members of the major facilitator superfamily of multidrug efflux pumps. The remaining four genes, named pvuBCDE, encode proteins strongly homologous to Escherichia coli FecBCDE, respectively, which are components of the ATP-binding cassette transporter system for ferric dicitrate. Reverse transcriptase PCR analysis revealed that these transport genes are transcribed as a single mRNA with the upstream genes, psuA and pvuA. Phenotypic comparison between the wild-type strain and its targeted gene disruptants supported the biological functions for the respective operons that were expected on the basis of the homology search.

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“…Rhizoferrin seems to be a characteristic siderophore of a variety of fungal species like Rhizopus, Mucor, Phycomyces, Chaetostylum, Absidia, Cokeromyces, Cunninghamella, Mycotypha, and Mortierella (Winkelmann, 1992;Thieken and Winkelmann, 1992).…”

Section: Agrobacterium Tumefaciens; Dihydroxybenzoic Acid By Erwinia mentioning

confidence: 99%

Siderophore Production by Microorganisms Isolated From a Podzol Soil Profile

Ahmed

Holmström

2014

Geomicrobiology Journal

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Siderophore producing bacteria/actinobacteria and fungi were isolated from O-(organic), E-(eluvial), B-(upper illuvial), and C-(parent material) horizons of podzol soil. Siderophores were isolated and hydroxamate type siderophores were detected and quantitated by high-performance liquid chromatography coupled to electrospray ionization mass spectrometry. The molecular identification of siderophore producing isolates showed that there was a high diversity of fungal and bacterial/actinobacterial species throughout the soil profile. The isolated bacteria/actinobacteria showed different abilities in the production of ferrioxamines (E, B, G and D). Moreover, the isolated fungal species showed great variety in the production of ferrichromes, coprogens and fusarinines.

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Rhizoferrin: A complexone type siderophore of the mocorales and entomophthorales (Zygomycetes)

Cited by 59 publications

References 4 publications

Characterization of the Siderophore ofFrancisella tularensisand Role offslAin Siderophore Production

Characterization of the Siderophore ofFrancisella tularensisand Role offslAin Siderophore Production

Identification and Characterization of Genes Required for Biosynthesis and Transport of the Siderophore Vibrioferrin in Vibrio parahaemolyticus

Siderophore Production by Microorganisms Isolated From a Podzol Soil Profile

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