Rhodococcus UKMP-5M, an endogenous lipase producing actinomycete from Peninsular Malaysia

Nagarajan, J.; Nawawi, Mohd; Ibrahim, Ab Latif

doi:10.2478/s11756-013-0308-x

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…and Halomonas spp. are reported to produce lipase, , while based on a current study, Tetracladium genomes are enriched for esterase and pectate lyase domains . Lipase and esterase are important enzymes for PBSA degradation. , Furthermore, Tetracladium spp.…”

Section: Resultsmentioning

confidence: 81%

Back to the Future: Decomposability of a Biobased and Biodegradable Plastic in Field Soil Environments and Its Microbiome under Ambient and Future Climates

Purahong

Wahdan

Heinz

et al. 2021

Environ. Sci. Technol.

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Decomposition by microorganisms of plastics in soils is almost unexplored despite the fact that the majority of plastics released into the environment end up in soils. Here, we investigate the decomposition process and microbiome of one of the most promising biobased and biodegradable plastics, poly(butylene succinate-co-adipate) (PBSA), under field soil conditions under both ambient and future predicted climates (for the time between 2070 and 2100). We show that the gravimetric and molar mass of PBSA is already largely reduced (28–33%) after 328 days under both climates. We provide novel information on the PBSA microbiome encompassing the three domains of life: Archaea, Bacteria, and Eukarya (fungi). We show that PBSA begins to decompose after the increase in relative abundances of aquatic fungi (Tetracladium spp.) and nitrogen-fixing bacteria. The PBSA microbiome is distinct from that of surrounding soils, suggesting that PBSA serves as a new ecological habitat. We conclude that the microbial decomposition process of PBSA in soil is more complex than previously thought by involving interkingdom relationships, especially between bacteria and fungi.

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Section: Resultsmentioning

confidence: 81%

Back to the Future: Decomposability of a Biobased and Biodegradable Plastic in Field Soil Environments and Its Microbiome under Ambient and Future Climates

Purahong

Wahdan

Heinz

et al. 2021

Environ. Sci. Technol.

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“…Tween 20 (Sigma-Aldrich) was an adequate sub strate for simple and fast detection of microbial lipolytic activity on agar plate. White-like precipitate appears around the colony having lipase activity (Nagarajan et al, 2014).…”

Section: Lipase Activity Assaysmentioning

confidence: 99%

Biodegradation of animal fats and vegetable oils by Rhodococcus erythropolis PR4

Kis

Laczi

Zsíros

et al. 2015

International Biodeterioration & Biodegradation

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The alkane (pristane) degradation capacity o f Rhodococcus erythropolis PR4 (NBRC 100887), isolated from marine environment, was previously observed. In this study, the ability of this strain for biodegradation of various animal fats, such as pig lards and poultry fats as well as butter, margarine and sunflower cooking oil was studied. Bioconversion of fats and oil was determined as methyl-ester (FAME) derivatives by GC-MS. R. erythropolis PR4 strain could utilize all substrates tested but the bioconversion rate and efficacies varied. The optimum pH for decomposition of pig lard and poultry fat was 8.5, respectively. Addition of carbonate to the media dramatically improved the efficiency o f the process via stabilization of pH o f the fermentation. Biotransformation of poultry fat was complete in four days and around 80% conversion was reached in the case of pig lard in media containing carbonate. The extracellular lipase activity of the R. erythropolis PR4 strain was also demonstrated by various techniques. The results suggest the R. erythropolis PR4 strain studied is a promising candidate in bioremediation/bioconversion of fatcontaining wastes within a relatively short time.

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“…Understanding the influence of variation in these factors is important to provide the optimal conditions that are essential for microbial metabolism in order to degrade the targeted pollutant in the environment [10]. A number of tropical bacteria have been identified with potential in the biodegradation of waste cooking oils, grease and kitchen waste [20]. Nzila et al [17] reported that a bacterial consortium (including Stenotrophomonas rhizophila, Sphingobacterium sp., Pseudomonas libanensis, P. poae and P. aeruginosa) isolated from wastewater in a Saudi Arabian city could degrade 90% of oil within 7-9 days.…”

Section: Introductionmentioning

confidence: 99%

The Use of Response Surface Methodology as a Statistical Tool for the Optimisation of Waste and Pure Canola Oil Biodegradation by Antarctic Soil Bacteria

Zahri

Zulkharnain

Gómez-Fuentes

et al. 2021

Life

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Hydrocarbons can cause pollution to Antarctic terrestrial and aquatic ecosystems, both through accidental release and the discharge of waste cooking oil in grey water. Such pollutants can persist for long periods in cold environments. The native microbial community may play a role in their biodegradation. In this study, using mixed native Antarctic bacterial communities, several environmental factors influencing biodegradation of waste canola oil (WCO) and pure canola oil (PCO) were optimised using established one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. The factors include salinity, pH, type of nitrogen and concentration, temperature, yeast extract and initial substrate concentration in OFAT and only the significant factors proceeded for the statistical optimisation through RSM. High concentration of substrate targeted for degradation activity through RSM compared to OFAT method. As for the result, all factors were significant in PBD, while only 4 factors were significant in biodegradation of PCO (pH, nitrogen concentration, yeast extract and initial substrate concentration). Using OFAT, the most effective microbial community examined was able to degrade 94.42% and 86.83% (from an initial concentration of 0.5% (v/v)) of WCO and PCO, respectively, within 7 days. Using RSM, 94.99% and 79.77% degradation of WCO and PCO was achieved in 6 days. The significant interaction for the RSM in biodegradation activity between temperature and WCO concentration in WCO media were exhibited. Meanwhile, in biodegradation of PCO the significant factors were between (1) pH and PCO concentration, (2) nitrogen concentration and yeast extract, (3) nitrogen concentration and PCO concentration. The models for the RSM were validated for both WCO and PCO media and it showed no significant difference between experimental and predicted values. The efficiency of canola oil biodegradation achieved in this study provides support for the development of practical strategies for efficient bioremediation in the Antarctic environment.

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Rhodococcus UKMP-5M, an endogenous lipase producing actinomycete from Peninsular Malaysia

Cited by 9 publications

References 38 publications

Back to the Future: Decomposability of a Biobased and Biodegradable Plastic in Field Soil Environments and Its Microbiome under Ambient and Future Climates

Back to the Future: Decomposability of a Biobased and Biodegradable Plastic in Field Soil Environments and Its Microbiome under Ambient and Future Climates

Biodegradation of animal fats and vegetable oils by Rhodococcus erythropolis PR4

The Use of Response Surface Methodology as a Statistical Tool for the Optimisation of Waste and Pure Canola Oil Biodegradation by Antarctic Soil Bacteria

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