Rhodovulum algae sp. nov., isolated from an algal mat

Abstract

A reddish-brown-pigmented, phototrophic bacterium, designated strain JA877 T , was isolated from a brown algae mat sample collected from Jalandhar beach, Gujarat, India. On the basis of the 16S rRNA gene sequence, strain JA877 T belongs to the class Alphaproteobacteria and is closely related to the type strains Rhodovulum viride JA756 T (99.0 %), Rhodovulum sulfidophilum Hansen W4 T (98.9 %), Rhodovulum visakhapatnamense JA181 T (98.8 %), Rhodovulum kholense JA297 T (97.5 %) and Rhodovulum salis JA746 T (97.0). However, strain JA877 T showed only 20-45 % relatedness with its phylogenetic neighbours and had a DT m between 5.8 and 7.0 C. The major respiratory quinone was ubiquinone-10 (Q10), and the polar lipid profile was composed of the major components phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, two unidentified sulfolipids and five unidentified lipids. The major fatty acids were C 18 : 1 !5c, C 18 : 1 !7c/C 18 : 1 !6c, C 16 : 0 and C 18 : 0 . The DNA G+C content was 64.5 mol%. On the basis of 16S rRNA gene sequence analysis, physiological data, and chemotaxonomic and molecular differences, strain JA877 T is significantly different from other species of the genus Rhodovulum and represents a novel species, for which the name Rhodovulum algae sp. nov. is proposed. The type strain is JA877 T (=LMG 29228 T = KCTC 42963 T ).Members of genus Rhodovulum (Rdv.) are phototrophic, isolated from marine habitats and form a distinct monophyletic 16S rRNA gene cluster with no interspersing chemotrophs. At the time of writing, there were 19 species with validly published names assigned to this genus (Divyasree et al., 2016). In this study, we isolated a strain (JA877 T ) from a brown algae mat sample and found that the 16S rRNA gene sequence of the isolate was related to those of species of the genus Rhodovulum. Here we describe the phenotypic, physiological and chemotaxonomic features of this isolate.Strain JA877 T was isolated from a brown algae mat sample collected from Jalandhar beach, Gujarat, India (GPS positioning of the sample collection site: 20 709¢ N 70 985¢ E), on 21 December 2014. One gram brown algae mat was serially diluted [10-fold dilution in saline (0.9 % NaCl)] and plated on a Zobell's marine agar (MA) medium (Himedia, India, cat no. M384). Three distinct colony morphologies were observed from plates incubated at 30 C for 6 days. Reddish-brown-pigmented colonies were purified by subsequent streaking on the same medium. The purified isolate was designated strain JA877 T and further the strain was preserved as glycerol stocks and stored at 4 C. For routine culturing and for physiological tests, strain JA877 T was grown in a medium described previously (Srinivas et al., 2014) in 50 ml fully filled, screw-capped bottles incubated at 30 C under 200 W m À2 of illumination by a tungsten lamp for 7 days under anaerobic conditions. Genomic DNA was isolated and purified by the method described by Marmur (1961), and the G+C content (mol%) Abbreviations: ML, maximum-likelihood; MP, max...

Anianabacter salinae gen. nov., sp. nov. ASV31T, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern

“…This characteristic was observed in this study. Photorganoheterotrophy was also observed in Rhodovulum algae LMG 29228 T (as described by Ramaprasad et al [38]). Fermentative growth was not seen in any of the strains analysed, as described in [5].…”