Ribonuclease activity as a new prospective disease resistance marker in potato

Abstract

Disease resistance is an important characteristic for each variety of potato, and the search for pathogen resistance markers is one of the primary tasks of plant breeding. Higher plants possess a wide spectrum of enzymes catalyzing the hydrolysis of nucleic acids; it is believed that protection against pathogens is the most probable function of the enzymes. RNases are actively involved in several immune systems of higher plants, for example, systemic acquired resistance (SAR) and genetic silencing, hence RNase activity in plant leaves, as a relatively easily measured parameter, can serve as a good marker for the selection of pathogen resistant varieties. We have analyzed sixteen varieties of potatoes permitted for use on the territory of the Russian Federation and tested the correlation of the level of variety­specifc ribonuclease (RNase) activity with such economically valuable traits as maturity and resistance to viruses, late blight and common scab. In general, the level of RNase activity was variety­specifc, which was confrmed by very small values of average squared error for the majority of tested varieties. We have detected a statistically signifcant positive correlation of RNase activity in potato leaves with increased resistance of varieties to phytopathogenic viruses, a negative correlation with resistance to scab and an absence of a signifcant connection with maturity and resistance to late blight, regardless of the organ affected by the oomycete. Thus, the level of RNase activity in potato leaves can be used as a selective marker for resistance to viruses, while varieties with increased RNase activity should be avoided when selecting resistance to scab.

By Modulating the Hormonal Balance and Ribonuclease Activity of Tomato Plants Bacillus subtilis Induces Defense Response against Potato Virus X and Potato Virus Y

“…It should be noted that the ribonuclease activity decreased in the PVY-susceptible VS variety and increased in the PVY-resistant Ural variety (Figure 4). Our results are consistent with the data obtained by other authors, in which a high correlation was found between the ribonuclease activity and the resistance to viruses in different potato varieties [26].…”

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

“…Thus, for buckwheat varieties Roksolana and Kara-Dag with different resistance to the buckwheat burn virus (BBV), a positive correlation between resistance to virus and RNase activity was shown ( Sindarovska et al, 2014 ). Trifonova et al (2018) have proposed to use the level of RNase activity in potato leaves as a selective marker for resistance to viruses.…”

“…Pseudomonas putida A3 ( Yang et al, 2012 ) and B. pumilus 7P/3-19 ( Sharipova et al, 2015 ) were shown to cleave viral particles in the juice from TMV-infected tobacco plants. The strong positive correlation was shown between the RNase activity in different potato varieties and their resistance to PVX, PVY, PVM, and PVS ( Trifonova et al, 2018 ). Expression of RNase gene PAC1 from Schizosaccharomyces pombe in soybean plants led to a significantly higher level of uninfected with prevalent in soybean-growing regions of China soybean mosaic virus (SMV) SC3 strain under the field conditions, compared with the areas where non-transformed plants were grown ( Yang et al, 2019 ).…”

Endophytic Bacillus bacteria with RNase activity in the resistance of potato plants to viruses

“…It is of interest to develop antiviral biocontrol agents based on PGPR isolates having high RNase activity or the preparation of RNase itself to protect plants from viral infection, taking into account that the majority of phytoviruses are RNA viruses (Sharipova et al, 2015) as well as the generation of genetically modified plants expressing RNase genes (Trifonova et al, 2004). It has been found that potato plants expressing Serratia marcescens nuclease display enhanced resistance to pathogens (Trifonova et al, 2018).…”