Ribosomes in Peripheral and Presynaptic Domains of Axons

Martin, Rainer de

doi:10.1007/978-1-4615-5405-9_110

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…More recently, contacts between keratinocytes and sensory afferents have also been observed at the ultrastructural level [16]. In line with previous studies [28; 32], we also detected components of the translational machinery and mitochondrial proteins in nerve terminals, underscoring their capacity for energy metabolism [13] and protein synthesis. This agrees with observations that ribosomes are not only present in the cell body but also in the terminals of sensory neurons [59].…”

Section: Discussionsupporting

confidence: 90%

Proteomic analysis of isolated nerve terminals from Na_V1.9 knockout mice reveals pathways relevant for neuropathic pain signalling

Rawat,

Vu,

Erbacher

et al. 2024

Preprint

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Neuropathic pain substantially affects the mental and physical well-being of patients and magnifies the socio-economic burden on the healthcare system. It is important to understand the molecular mechanisms underlying chronic pain to effectively target it. To investigate peripheral mechanisms relevant to pain signaling, we isolated nerve terminals from mouse footpads. The isolated peripheral terminals contain both pre- and post-synaptic proteins and are deficient in keratin and histone in both mice and humans. We detected the protein translational machinery and mitochondria in nerve terminals and observed that they were capable of endocytosis. An unbiased proteomic analysis of nerve terminals from footpads of NaV1.9 knockout mice shows dysregulation of the p38 mitogen-activated protein kinase (MAPK) and extracellular regulated kinase 1/2 (ERK1/2) pathways, and of protein components involved in translation and energy metabolism. Isolation of human nerve terminals from skin punch biopsies, validated by proteomic analysis, highlights the broad and translational value of our approach. Our study thus reveals peripheral signaling mechanisms implicated in pain perception.

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Section: Discussionsupporting

confidence: 90%

Proteomic analysis of isolated nerve terminals from Na_V1.9 knockout mice reveals pathways relevant for neuropathic pain signalling

Rawat,

Vu,

Erbacher

et al. 2024

Preprint

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“…Besides the transcripts for the hormones, the mRNA encoding the light NF protein was also detected in the axons of the same neurons (232). Ribosomes have not been reported in neurohypophysial axons of control rats, but they are present in the hypertrophic vasopressin-containing axons following chronic stimulation by a salt-loading regime (223). In addition, the mRNAs encoding olfactory receptors and the olfactory marker protein were identified in the axons of olfactory receptor neurons (70,268,307,328).…”

Section: Vertebrate Axonsmentioning

confidence: 95%

Local Gene Expression in Axons and Nerve Endings: The Glia-Neuron Unit

Giuditta¹,

Chun²,

Eyman³

et al. 2008

Physiological Reviews

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Neurons have complex and often extensively elongated processes. This unique cell morphology raises the problem of how remote neuronal territories are replenished with proteins. For a long time, axonal and presynaptic proteins were thought to be exclusively synthesized in the cell body, which delivered them to peripheral sites by axoplasmic transport. Despite this early belief, protein has been shown to be synthesized in axons and nerve terminals, substantially alleviating the trophic burden of the perikaryon. This observation raised the question of the cellular origin of the peripheral RNAs involved in protein synthesis. The synthesis of these RNAs was initially attributed to the neuron soma almost by default. However, experimental data and theoretical considerations support the alternative view that axonal and presynaptic RNAs are also transcribed in the flanking glial cells and transferred to the axon domain of mature neurons. Altogether, these data suggest that axons and nerve terminals are served by a distinct gene expression system largely independent of the neuron cell body. Such a local system would allow the neuron periphery to respond promptly to environmental stimuli. This view has the theoretical merit of extending to axons and nerve terminals the marginalized concept of a glial supply of RNA (and protein) to the neuron cell body. Most long-term plastic changes requiring de novo gene expression occur in these domains, notably in presynaptic endings, despite their intrinsic lack of transcriptional capacity. This review enlightens novel perspectives on the biology and pathobiology of the neuron by critically reviewing these issues.

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Ribosomes in the squid giant axon

Bleher

Martin

2001

Neuroscience

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Ribosomes in Peripheral and Presynaptic Domains of Axons

Cited by 3 publications

References 10 publications

Proteomic analysis of isolated nerve terminals from Na_V1.9 knockout mice reveals pathways relevant for neuropathic pain signalling

Proteomic analysis of isolated nerve terminals from Na_V1.9 knockout mice reveals pathways relevant for neuropathic pain signalling

Local Gene Expression in Axons and Nerve Endings: The Glia-Neuron Unit

Ribosomes in the squid giant axon

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Ribosomes in Peripheral and Presynaptic Domains of Axons

Cited by 3 publications

References 10 publications

Proteomic analysis of isolated nerve terminals from NaV1.9 knockout mice reveals pathways relevant for neuropathic pain signalling

Proteomic analysis of isolated nerve terminals from NaV1.9 knockout mice reveals pathways relevant for neuropathic pain signalling

Local Gene Expression in Axons and Nerve Endings: The Glia-Neuron Unit

Ribosomes in the squid giant axon

Contact Info

Product

Resources

About

Proteomic analysis of isolated nerve terminals from Na_V1.9 knockout mice reveals pathways relevant for neuropathic pain signalling

Proteomic analysis of isolated nerve terminals from Na_V1.9 knockout mice reveals pathways relevant for neuropathic pain signalling