Rice Diversity Panel Mapping for Identifying Genetics Behind Leaf Vein Density Trait in Rice (<i>Oryza sativa</i> L.)

Abstract

Abstract:A screen of rice genotypes was carried out on a total of 327 genotypes of the rice diversity panel for leaf vein density. Identifying locations of genes that impart to leaf vein density in a quantitative way should capable the using of these genes in plant breeding and accelerate producing C4 rice plant. Finding of genotypes of rice that have less distances between veins is still the challenge of the plant breeders. Screen was shown significant variations in leaf vein density. Quantitative trait loci (QTLs) for the leaf vein density trait were identified by using Efficient Mixed Model Analysis (EMMA). QTLs were considered reportable if they had P values (below 0.0001). The most significant Single nucleotide polymorphisms (SNP) associations (EMMA 1.3, EMMA 1.8 and EMMA 10.1) were in each of the rice chromosomes 1 and 10 respectively. All genes positioned 200 kb around associations were selected. The candidacy of the most promising were NADP-dependent malic enzyme, chloroplast precursor (LOC_Os01g09320; ras-related protein, putative, expressed (LOC_Os01g51700); 60S acidic ribosomal protein (LOC_Os01g09510); Auxin-responsive Aux/IAA gene family member (LOC_Os01g09450; myb-related transcription activator, putative, expressed (LOC_Os01g09280); glycine-rich protein (LOC_Os01g09246); phosphofructokinase (LOC_Os01g09570); oxidoreductase, short chain dehydrogenase/reductase family (SDR) LOC_Os10g31780), which have been expressed in leaf tissue and requisite to be investigated further.