Rice dwarf phytoreovirus segment S12 transcript is tricistronic in Vitro

Suzuki, Nobuhiro; Sugawara, Mikiko; Kusano, Tomonobu

doi:10.1016/0042-6822(92)90279-x

Cited by 23 publications

(16 citation statements)

References 32 publications

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“…Fluorogram of SDS-PAGE of translation products in wheatgerm extracts programmed by synthetic transcripts generated from full-length cDNA clones of RDV genome segments, cDNA clones used for in vitro transcription were: 1 pRS4; 2 pRS5; 3 pRS6; 4 pRS7; 5 pRS8; 6 pRS9; 7 pRS10; 8 pRS11M; 9 pRS12. The products of S11 (P1 la, 8) and S12 (P12, 9) were reported previously [17,19] Concerning the assignment of the S9-coded product, a 45 k outer capsid protein has been proposed [9]. On the other hand, Uyeda et al [23] and Fukumoto et al E3] have suggested that $9 encodes a non-structural protein.…”

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confidence: 87%

“…Of these 12 genome segments, 11 have already been sequenced [3,10,12,13,17,[19][20][21][22][23][24], and only the sequence of the second largest segment $2 has yet to be determined. These sequence analyses have shown that the RDV segments each have a single large open reading frame (ORF), except for $12 [19]. With regard to gene products, only $1-, $3-, $7-, $8-, S11-, and S12-coded proteins have been found either or both in vitro and in vivo [10, 12, t7, 19, 20, 22], whereas no other virus-coded polypeptides have been identified.…”

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confidence: 99%

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In vitro translation of rice dwarf phytoreovirus genome segments S4 to S10

Suzuki¹

1993

Archives of Virology

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The individual synthetic plus-strand transcripts from full-length cDNAs of rice dwarf phytoreovirus genome segments S4 to S10 directed the in vitro synthesis of predominant polypeptides, whose migration positions in SDS-polyacrylamide gel indicated molecular masses of 83 k (P4), 89 k (P5), 56 k (P6), 58 k (P7), 43 k (P8), 49 k (P9), and 35 k (P10), respectively. P7 and P8 had the same mobilities as those of the structural proteins, supporting the previous observations. The other proteins (P4, P6, P10) as well as P9, which had been considered an outer capsid protein, are probably nonstructural proteins, since no polypeptides with similar migration behavior were found in a purified RDV preparation. It remains to be confirmed biochemically whether S5 encodes the outer layer protein.

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confidence: 87%

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confidence: 99%

In vitro translation of rice dwarf phytoreovirus genome segments S4 to S10

Suzuki¹

1993

Archives of Virology

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“…The nucleotide (nt) sequences of WTV segments S5, S7-8, S10-12 [7][8][9][10][11], RGDV segments S1, S2, S3, S5, S8-12 [12][13][14][15][16], and complete nt sequences of RDV [1,6,17] segments have been reported. The biological functions of some viral proteins have been assigned and characterized [12,[18][19][20][21][22][23][24][25][26][27]. In this study we determine the complete sequences and putative open reading frames of six segments (S5, S6, S8, S10-12) of a newly described phytoreovirus.…”

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confidence: 99%

Molecular analysis of six segments of Tobacco leaf enation virus, a novel phytoreovirus from tobacco

2007

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Tobacco leaf enation virus (TLEV) is a putative member of the genus Phytoreovirus within the family Reoviridae. Previous western blot analysis of structural viral proteins (apparent molecular weights of 93 kDa; 58 kDa; 48 kDa; 39 kDa and 36 kDa) associated with TLEV, isolated from infected tobacco in South Africa, suggested that these proteins may correspond to structural Wound tumor virus (WTV) proteins. To further establish the nature of this novel virus disease phenotype in tobacco, molecular characterization of six dsRNA components was undertaken. Full-length cDNA clones were obtained by an optimized modified single-primer amplification sequence-independent dsRNA cloning method. Results of this study revealed the conserved terminal sequence: 5'GG(U/C)...UGAU 3' of segments S6-S12, while adjacent to these conserved terminal sequences are imperfect inverted repeats (7-15 bp in length), both features being common to reoviruses. The complete nucleotide sequences of segments S5 (2,610 bp), S7 (1,740 bp), S8 (1,439 bp), S10 (1,252 bp), S11 (1,187 bp) and S12 (836 bp) were determined. Comparison of full-length nucleotide sequences with corresponding segments of other phytoreoviruses, Rice gall dwarf virus (RGDV), Rice dwarf virus (RDV) and WTV has shown nucleotide and predicted amino acid identities within the range of 30-60%. TLEV consistently shows a higher identity to WTV than to other phytoreovirus species where sequence data is available. Each segment had a single predicted open reading frame encoding proteins with calculated molecular weights of S5 (90.6 kDa); S7 (58.1 kDa); S8 (47.7 kDa); S10 (39.8 kDa); S11 (35 kDa) and S12 (19.5 kDa). The relatively low nucleotide and amino acid identity to other members of the genus demonstrates that TLEV is a novel phytoreovirus, distinct from the only other reported dicotyledenous-infecting WTV and is the first phytoreovirus reported to emerge in Africa.

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“…Polycistronic genes have been reported in viral genomes, where different protein messages may lie inphase side by side, or alternatively, on overlapping frames (Dinesh-Kumar et al 1992;Jacks et al 1988;Kirchner et al 1992;Lamb and Choppin 1979;Lamb and Horvath 1991;Montell et al 1982;Ray et al 1992;Suzuki et al 1992). The benefit of carrying different genes on overlapping frames is twofold: (1) it is a useful strategy to save space in microorganisms with a short gehome, like viruses and (2) it is a way to keep the expression of the overlapping proteins under the same regulatory control.…”

Section: Introductionmentioning

confidence: 99%

Investigating hypothetical products from noncoding frames (HyPNoFs)

Facchiano¹

1995

J Mol Evol

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Hypothetical Products from Noncoding Frames (i.e., HyPNoFs) are hypothetical, not-coded proteins, translated from alternate reading frames (i.e., coding + 1 and coding + 2) of cDNAs. HyPNoFs of CD4, PKC, oncostatin, bcl-2 proto-oncogene, tumor suppressor p53, cystic fibrosis transmembrane regulator (CFTR), and tumor necrosis factors alpha and beta were searched as query sequences vs the SWISS-PROT data bank. Homology searchers carried out revealed that hypothetical products (i.e., HyPNoFs) may share high similarity with real protein products actually coded. Sequence similarity of hypothetical products to real proteins is sometimes very high, suggesting common conformational features, according to the Sander and Schneider cutoff value. This finding supports the hypothesis that eukaryotic DNA, currently considered to be monocistronic, might occasionally have polycistronic regions, carrying different protein messages on overlapping frames. As yet, polycistronic genes have been observed in viral genomes only. The presence of polycistronic regions in eukaryotic genes is likely reminiscent of an ancient strategy, rather than a present feature of the genome in eukaryotes. These data suggest that thorough investigation of HyPNoFs is likely to improve our ability to trace genes' evolution and to investigate structure-function relationships of protein and DNA sequences.

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Rice dwarf phytoreovirus segment S12 transcript is tricistronic in Vitro

Cited by 23 publications

References 32 publications

In vitro translation of rice dwarf phytoreovirus genome segments S4 to S10

In vitro translation of rice dwarf phytoreovirus genome segments S4 to S10

Molecular analysis of six segments of Tobacco leaf enation virus, a novel phytoreovirus from tobacco

Investigating hypothetical products from noncoding frames (HyPNoFs)

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