Rice ORMDL Controls Sphingolipid Homeostasis Affecting Fertility Resulting from Abnormal Pollen Development

Chueasiri, Chutharat; Chunthong, Ketsuwan; Pitnjam, Keasinee; Chakhonkaen, Sriprapai; Sangarwut, Numphet; Sangsawang, Kanidta; Suksangpanomrung, Malinee; Michaelson, Louise V.; Napier, Johnathan A.; Muangprom, Amorntip

doi:10.1371/journal.pone.0106386

Cited by 27 publications

(15 citation statements)

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“…Suppression of LOC_Os07 g26940 ORMDL or all three rice ORMDLs lead to abnormal pollen development resulting in male sterility. In contrast with previous findings in yeasts and mammals, where decreased expression of ORMDLs leads to increased production of sphingolipids and ceramides, in tms2 sterile plants, where expression of LOC_Os07g26940 ORMDL is decreased, the ceramide levels are also decreased (66). It needs to be determined whether ORMDL-mediated regulation of sphingolipid biosynthesis could play a role in production of gametes, not only in plants, but also in other organisms.…”

Section: -Ketodihydrosphinganine Reductasecontrasting

confidence: 62%

The role of ORMDL proteins, guardians of cellular sphingolipids, in asthma

Paulenda

Dráber

2016

Allergy

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To cite this article: Paulenda T, Draber P. The role of ORMDL proteins, guardians of cellular sphingolipids, in asthma. AbstractA family of widely expressed ORM-like (ORMDL) proteins has been recently linked to asthma in genomewide association studies in humans and extensively explored in in vivo studies in mice. ORMDL proteins are key regulators of serine palmitoyltransferase, an enzyme catalyzing the initial step of sphingolipid biosynthesis. Sphingolipids play prominent roles in cell signaling and response to stress, and they affect the mechanistic properties of cellular membranes. Deregulation of sphingolipid biosynthesis and their recycling has been proven to support and even cause several diseases including allergy, inflammation, and asthma. ORMDL3, the most extensively studied member of the ORMDL family, has been shown to be important for endoplasmic reticulum homeostasis by regulating the unfolded protein response and calcium response. In immune cells, ORMDL3 is involved in migration and in the production of proinflammatory cytokines. Furthermore, changes in the expression level of ORMDL3 are important in allergen-induced asthma pathologies. This review focuses on functional aspects of the ORMDL family proteins, which may serve as new therapeutic targets for the treatment of asthma and some other life-threatening diseases.

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Section: -Ketodihydrosphinganine Reductasecontrasting

confidence: 62%

The role of ORMDL proteins, guardians of cellular sphingolipids, in asthma

Paulenda

Dráber

2016

Allergy

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“…ORM proteins were found to be negative regulators of SPT in yeast [7] . In rice, RNAi against ORM-like genes also led to abnormal pollen development confirming the importance of sphingolipids for male fertility [16] .…”

Section: Introductionmentioning

confidence: 83%

Plant sphingolipids: Their importance in cellular organization and adaption

Michaelson

Napier

Molino

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Sphingolipids and their phosphorylated derivatives are ubiquitous bio-active components of cells. They are structural elements in the lipid bilayer and contribute to the dynamic nature of the membrane. They have been implicated in many cellular processes in yeast and animal cells, including aspects of signaling, apoptosis, and senescence. Although sphingolipids have a better defined role in animal systems, they have been shown to be central to many essential processes in plants including but not limited to, pollen development, signal transduction and in the response to biotic and abiotic stress. A fuller understanding of the roles of sphingolipids within plants has been facilitated by classical biochemical studies and the identification of mutants of model species. Recently the development of powerful mass spectrometry techniques hailed the advent of the emerging field of lipidomics enabling more accurate sphingolipid detection and quantitation. This review will consider plant sphingolipid biosynthesis and function in the context of these new developments. This article is part of a Special Issue entitled: Plant Lipid Biology edited by Kent D. Chapman and Ivo Feussner.

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“…OsAP65, a rice aspartic protease, was essential for male fertility and played a important role in pollen germination and pollen tube growth [ 79 ]. Chueasiri et al [ 80 ] quantified gene expression in anthers of temperature-sensitive rice plants grown in controlled growth rooms (26 °C and 32 °C) for fertile and sterile conditions, the results indicated that plant orosomucoids-like proteins influenced sphingolipid homeostasis, and deletion of this gene affected spikelet fertility resulting from abnormal pollen development. The transcription factor bHLH142 was identified as a pivotal role in tapetal programmed cell death and pollen development during early meiosis in rice [ 81 ].…”

Section: Discussionmentioning

confidence: 99%

RNA-seq reveals differentially expressed genes of rice (Oryza sativa) spikelet in response to temperature interacting with nitrogen at meiosis stage

Yang

Chen

et al. 2015

BMC Genomics

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BackgroundRice (Oryza sativa) is one of the most important cereal crops, providing food for more than half of the world’s population. However, grain yields are challenged by various abiotic stresses such as drought, fertilizer, heat, and their interaction. Rice at reproductive stage is much more sensitive to environmental temperatures, and little is known about molecular mechanisms of rice spikelet in response to high temperature interacting with nitrogen (N).ResultsHere we reported the transcriptional profiling analysis of rice spikelet at meiosis stage using RNA sequencing (RNA-seq) as an attempt to gain insights into molecular events associated with temperature and nitrogen. This study received four treatments: 1) NN: normal nitrogen level (165 kg ha−1) with natural temperature (30 °C); 2) HH: high nitrogen level (330 kg ha−1) with high temperature (37 °C); 3) NH: normal nitrogen level and high temperature; and 4) HN: high nitrogen level and natural temperature, respectively. The de novo assembly generated 52,553,536 clean reads aligned with 72,667 unigenes. About 10 M reads were identified from each treatment. In these differentially expressed genes (DEGs), we found 151 and 323 temperature-responsive DEGs in NN-vs-NH and HN-vs-HH, and 114 DEGs were co-expressed. Meanwhile, 203 and 144 nitrogen-responsive DEGs were focused in NN-vs-HN and NH-vs-HH, and 111 DEGs were co-expressed. The temperature-responsive genes were principally associated with calcium-dependent protein, cytochrome, flavonoid, heat shock protein, peroxidase, ubiquitin, and transcription factor while the nitrogen-responsive genes were mainly involved in glutamine synthetase, transcription factor, anthocyanin, amino acid transporter, leucine zipper protein, and hormone. It is noted that, rice spikelet fertility was significantly decreased under high temperature, but it was more reduced under higher nitrogen. Accordingly, numerous spikelet genes involved in pollen development, pollen tube growth, pollen germination, especially sporopollenin biosynthetic process, and pollen exine formation were mainly down-regulated under high temperature. Moreover, the expression levels of co-expressed DEGs including 5 sporopollenin biosynthetic process and 7 pollen exine formation genes of NN-vs-NH were lower than that of HN-vs-HH. Therefore, these spikelet genes may play important roles in response to high temperature with high nitrogen and may be good candidates for crop improvement.ConclusionsThis RNA-seq study will help elucidate the molecular mechanisms of rice spikelet defense response to high temperature interacting with high nitrogen level.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2141-9) contains supplementary material, which is available to authorized users.

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Rice ORMDL Controls Sphingolipid Homeostasis Affecting Fertility Resulting from Abnormal Pollen Development

Cited by 27 publications

References 35 publications

The role of ORMDL proteins, guardians of cellular sphingolipids, in asthma

The role of ORMDL proteins, guardians of cellular sphingolipids, in asthma

Plant sphingolipids: Their importance in cellular organization and adaption

RNA-seq reveals differentially expressed genes of rice (Oryza sativa) spikelet in response to temperature interacting with nitrogen at meiosis stage

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