Rice peroxisomal ascorbate peroxidase knockdown affects ROS signaling and triggers early leaf senescence

Ribeiro, C. T. M.; Körbes, Ana Paula; Garighan, Julio de Andrade; Jardim-Messeder, Douglas; Carvalho, Fernanda Torres de; Sousa, Rachel H. V.; Caverzan, Andréia; Teixeira, Felipe Karam; Silveira, Joaquim Albenísio Gomes da; Margis‐Pinheiro, Márcia

doi:10.1016/j.plantsci.2017.07.009

Cited by 82 publications

(54 citation statements)

References 67 publications

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“…The interesting triple localization pattern of APX5 needs to be further clarified, as APX5 appears to be a shared component of antioxidant systems in all three energy organelles. Consistent with these findings, increased APX activities were reported during leaf senescence in pea peroxisomes, mitochondria and chloroplasts (Jim enez et al 1998;Palma et al 2006;Ribeiro et al 2017).…”

Section: Identification Of a Large Number Of Peroxisomal Proteinssupporting

confidence: 81%

Proteome analysis of peroxisomes from dark‐treated senescent Arabidopsis leaves

Pan

Reumann

Lisik

et al. 2018

JIPB

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Peroxisomes compartmentalize a dynamic suite of biochemical reactions and play a central role in plant metabolism, such as the degradation of hydrogen peroxide, metabolism of fatty acids, photorespiration, and the biosynthesis of plant hormones. Plant peroxisomes have been traditionally classified into three major subtypes, and in-depth mass spectrometry (MS)-based proteomics has been performed to explore the proteome of the two major subtypes present in green leaves and etiolated seedlings. Here, we carried out a comprehensive proteome analysis of peroxisomes from Arabidopsis leaves given a 48-h dark treatment. Our goal was to determine the proteome of the third major subtype of plant peroxisomes from senescent leaves, and further catalog the plant peroxisomal proteome. We identified a total of 111 peroxisomal proteins and verified the peroxisomal localization for six new proteins with potential roles in fatty acid metabolism and stress response by in vivo targeting analysis. Metabolic pathways compartmentalized in the three major subtypes of peroxisomes were also compared, which revealed a higher number of proteins involved in the detoxification of reactive oxygen species in peroxisomes from senescent leaves. Our study takes an important step towards mapping the full function of plant peroxisomes.

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Section: Identification Of a Large Number Of Peroxisomal Proteinssupporting

confidence: 81%

Proteome analysis of peroxisomes from dark‐treated senescent Arabidopsis leaves

Pan

Reumann

Lisik

et al. 2018

JIPB

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“…Plant senescence is primarily determined by the development stage and is strongly influenced by both internal and environmental signals. The perception of these different signals is mediated by the ROS, which act as a signaling molecule to regulate the gene expression of senescence when at low levels and as a cytotoxic molecule when at high levels . In the present study, the contents of both hydrogen peroxide (H 2 O 2 ) and hydroxyl radical (·OH) increased in control fruit peel during postharvest storage (Fig.…”

Section: Resultsmentioning

confidence: 50%

Comparative volatile compounds and primary metabolites profiling of pitaya fruit peel after ozone treatment

Zhang

Zhu

et al. 2018

J Sci Food Agric

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BACKGROUND: Ozone treatment can effectively inhibit fruit decay in many fruits during postharvest storage. However, little information is available for pitaya fruit. RESULTS:Ozone treatment significantly reduced the decay rate and induced the enzyme activities of peroxidase and polyphenol oxidase, and also reduced the levels of reactive oxygen species. In total, 103 metabolites were detected and changed the content after ozone treatment, including 54 primary metabolites and 49 aromatic compounds. After significance and importance analysis, 37 metabolites were important. Some metabolites were induced by peel senescence to respond to senescence stress, including D-fructose, D-glucose, mannose, inositol, galactonic acid, ethanedioic acid and stearic acid. Some metabolic products of peel senescence were reduced by ozone treatment, including D-arabinose, glucaric acid, galacturonic acid, 1-hexanol, senescence and decay, including malic acid, succinic acid, pentenoic acid, eicosanoic acid, 2-hexenal, hexanal, 2-heptenal, 4-heptenal, 2-octenal and nitro m-xylene. CONCLUSION: Ozone treatment significantly reduced decay and prolonged shelf-life without reducing fruit quality. In total, 37 metabolites might play an important role in ozone delayed fruit decay. 4-ethylcyclohexanol, -linalool, palmitoleic acid and 2-hydroxy-cyclopentadecanone. Some metabolites induced by ozone treatment might play a vital role in delaying the RESULTS AND DISCUSSION Decay rateDuring the postharvest storage, fruit decay is the main factor leading to fruit loss. In pitaya fruit, decay generally occurs near the pedicle and then the disease spot expands gradually, which results in a fruit shelf-life of only 7-10 days at 25 ∘ C. To test the effect of ozone in pitaya, we screened the concentration J Sci Food Agric 2019; 99: 2610-2621

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“…It suggested that H 2 O 2 might play the mediated role in the senescence of pitaya peel. Previous study reported that H 2 O 2 was a cytotoxic molecule at high levels and a signaling molecule at low levels that regulates the gene expression associated with stress responses [30]. In this study, red light irradiation might not only significantly delay fruit senescence and decay of pitaya but also induce fruit resistance by reducing H 2 O 2 content.…”

Section: H 2 O 2 Mediated the Peel Senescence Of Pitayamentioning

confidence: 67%

Deciphering the Metabolic Pathways of Pitaya Peel after Postharvest Red Light Irradiation

Gao

Zhang

et al. 2020

Metabolites

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Red light irradiation can effectively prolong the shelf-life of many fruit. However, little is known about red light-induced metabolite and enzyme activities. In this study, pitaya fruit was treated with 100 Lux red light for 24 h. Red light irradiation significantly attenuated the variation trend of senescence traits, such as the decrease of total soluble solid (TSS) and TSS/acidity (titratable acidity, TA) ratio, the increase of TA, and respiratory rate. In addition, the reactive oxygen species (ROS) related characters, primary metabolites profiling, and volatile compounds profiling were determined. A total of 71 primary metabolites and 67 volatile compounds were detected and successfully identified by using gas chromatography mass spectrometry (GC-MS). Red light irradiation enhanced glycolysis, tricarboxylic acid (TCA) cycle, aldehydes metabolism, and antioxidant enzymes activities at early stage of postharvest storage, leading to the reduction of H2O2, soluble sugars, organic acids, and C-6 and C-7 aldehydes. At a later stage of postharvest storage, a larger number of resistance-related metabolites and enzyme activities were induced in red light-treated pitaya peel, such as superoxide dismutase (SOD), ascorbate peroxidase (APX), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging, reducing power, fatty acids, and volatile aroma.

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Rice peroxisomal ascorbate peroxidase knockdown affects ROS signaling and triggers early leaf senescence

Cited by 82 publications

References 67 publications

Proteome analysis of peroxisomes from dark‐treated senescent Arabidopsis leaves

Proteome analysis of peroxisomes from dark‐treated senescent Arabidopsis leaves

Comparative volatile compounds and primary metabolites profiling of pitaya fruit peel after ozone treatment

Deciphering the Metabolic Pathways of Pitaya Peel after Postharvest Red Light Irradiation

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