Rice Straw as a Support for Immobilization of Microbial Lipase

Abstract

Candida rugosa lipase was covalently immobilized on rice straw activated with glutaraldehyde using poly(ethylene glycol) (PEG) as the stabilizing agent. The effects of PEG molecular weight and enzyme loading were studied according to a full 2(2) factorial design. Higher immobilization yields (>70%) were attained when the lipase loading was 95 units/mg of dry support, independent of PEG molecular weight. All derivatives showed high hydrolytic and synthetic activities. This work provides preliminary results on the use of agricultural residues as a support matrix for immobilizing lipase and on the application of the resulting derivatives to butyl butyrate synthesis as a study model.

Kinetics of synthesis of butyl butyrate by esterification and transesterification in supercritical carbon dioxide

“…5). Conversions observed for the synthesis of butyl butyrate were only 27% in heptane, 6 16% in cyclohexane, 8 and 9% in heptane when lipase supported on rice straw 11 was used. However, in ScCO 2 , conversions of 77% and 52% were obtained in only 15 min for synthesis of isoamyl acetate 21 and lavandulyl acetate, 24 respectively.…”

Pilot-Scale Production of Lipase Using Palm Oil Mill Effluent as a Basal Medium and Its Immobilization by Selected Materials

“…Lowcost support materials are preferred for industrial applications; they can be organic chitin, chitosan or inorganic CaCO 3 26 . The organic support materials could come from lignocellulosic agro-industrial wastes, such as sawdust, wood chips/shavings, straw, spent grains 27 , rice straw 26,28 , cellulignin 29 , rice husk 30 , or coconut fiber 31 .…”

Estudo da influência do solvente, carboidrato e ácido graxo na síntese enzimática de ésteres de açúcares

“…Reações de síntese catalisadas por lipases imobilizadas na presença de PEG têm demonstrado um desempenho consistente e altamente satisfatório 23,25,26 devido, provavelmente, às interações hidrofóbicas entre o PEG, a enzima e o suporte.…”