Rifampin as a Selective Agent for Isolation of Oral Spirochetes

Leschine, Susan; Canale-Parola, E.

doi:10.1128/jcm.12.6.792-795.1980

Cited by 102 publications

(52 citation statements)

References 7 publications

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“…As the inocuJa contained an average of 0.5x10ŝ pirochetes/ml (range 0.1 x 10^-1.3 x 10m l), the maximal recovery on BHIA-SC with blood and GM-1 without blood was approximately 4%, while the maximal recovery on RGGA-SC and GM-I (both with blood) was 2%. These ftgures are of the same magnitude as those observed by Leschine and Canale-Parola (1980) by use of GM-1 medium with 2 ^g/ml rifampicin. Under the eonditions of the present experiment neither Hardy I nor PPLO-Socransky promoted growth of oral spirochetes wiiich is in contrast to the fmdings of Hardy et al 1963 andSocransky et al 1969.…”

Section: Resultssupporting

confidence: 78%

Evaluation of serum‐containing substrates for cultivation of oral spirochetes

Fiehn

Frandsen

1984

J of Periodontal Research

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The aim of this study was to evaluate eight different solid substrates with rabbit serum and ±5% blood for suitability to support growth of oral spirochetes in subgingival plaque samples from patients with advanced destructive periodontitis. In order to inhibit growth of other bacteria, nine antibiotics were added to the substrates singly or in combinations. Four of the tested substrates supported growth of oral spirochetes, BHIA‐SC and GM‐1 (both with blood), yielding the best results with a recovery of about 4%. The antibiotics best suited for the suppression of other bacteria were rifampicin, polymyxin B, and nalidixic acid.

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Section: Resultssupporting

confidence: 78%

Evaluation of serum‐containing substrates for cultivation of oral spirochetes

Fiehn

Frandsen

1984

J of Periodontal Research

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“…he GM-1 medium without blood was, how-^e r, almost selective for spirochetes with one endoflagella as spirochetes with more endo-flagella only sporadically occurred. This is in contrast to the findings of LESCHINE & CANALE-PAROLA (16). Only two media, RGCA-SC and MGM-1 (both with blood), permitted growth of all types of spirochetes observed in the primary samples.…”

Section: Discussioncontrasting

confidence: 97%

“…All six substrates contained 0.7% Noble agar, and had rifampicin (Sigma) 2 Hg/ml and polymyxin B (Sigma) 800 U/ml added by filtersterilization after autoclavation. The rifampicin stock solution was made according to LESCHINE & CANAI.E-PAROLA (16).…”

Section: Methodsmentioning

confidence: 99%

“…Several reports on cultivation of oral spirochetes have emerged during the two last decades (11)(12)(13)(14)(15). Most of the cultivation substrates used supported only growth of small spirochetes (11)(12)(13)(14), but LESCHINE & CANALE-PAROLA (16) found that the GM-1 medium Tiade the isolation of both small-and medium-sized oral spirochetes possible.…”

Section: Several Darkfield Microscopic Investigations Oarten and Helldenmentioning

confidence: 99%

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Cultivation on solid media of spirochetes in subgingival plaque from advanced marginal periodontitis in humans

Fiehn

Westergaard

1984

European J Oral Sciences

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– Six solid substrates were compared for suitability to support growth of spirochetes from 18 subgingival plaque samples in patients with advanced marginal periodontitis. The following substrates with rabbit serum were tested: BHIA‐SC + blood, RGCA‐SC + blood, GM‐1 ±blood and MGM‐1 ±blood. All media contained rifampicin and polymyxin B. After incubation in a glovebox (70% N2, 20% H2, 10% CO2) for 14 days spirochete colonies were semiquantitated and the colony morphologies described. The spirochetes in six of the primary samples and in the related colonies after cultivation were identified ultrastructurally by number of endoflagella. The results showed that the colony morphologies were more related to the substrate used than to the various types of spirochetes, and that individual colonies contained different types of spirochetes. The substrates MGM‐1, RGCA‐SC, and BHIA‐SC, all with blood, showed the highest recoveries of about 5%. Spirochetes with less than four endoflagella grew on all substrates, but the MGM‐1 + blood and RGCA‐SC + blood media seemed the most versatile as they showed growth of all types of spirochetes observed in the primary samples.

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“…Systems, Cockeysville, MD) supplemented with 5% heat-inactivated calf serum and 0.001% N-acetylmuramic acid. Treponema denticola was grown in fluid NOS medium as previously described [9]. The cultures were incubated at 35°C until they reached an optical density of 0.4 at 660 nm.…”

Section: Bacteroides Forsythus Was Grown In Brain Heart Infusion Brotmentioning

confidence: 99%

Detection of stress proteins in Porphyromonas gingivalis and other oral bacteria by Western immunoblotting analysis

Vayssier

1994

FEMS Microbiology Letters

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Detection of stress proteins in Porphyromonas gingivalis was investigated by SDS-PAGE and Western immunoblotting procedure using a polyclonal antibody (anti hsp60) and a monoclonal antibody (anti-Dnak). Results indicate that P. gingivalis can elicit a hsp60-like stress protein when submitted to different environmental stresses such as a heat shift from 35 degrees C to 43 degrees C, a pH drop from 7.2 to 6.0 or an increase in oxygen concentration. Virulent and non-virulent strains of P. gingivalis responded the same way. The other bacterial species tested also showed an increased synthesis of a GroEL-like protein after heat shock, as detected by the anti hsp60 antibody. However, the monoclonal anti-Dnak recognized an hsp70-like protein only in two of the tested species.

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Rifampin as a Selective Agent for Isolation of Oral Spirochetes

Abstract: Spirochetes indigenous to the healthy gingival crevice of the human mouth were isolated directly from colonies in agar medium containing rifampin as a selective agent.

Cited by 102 publications

References 7 publications

Evaluation of serum‐containing substrates for cultivation of oral spirochetes

Evaluation of serum‐containing substrates for cultivation of oral spirochetes

Cultivation on solid media of spirochetes in subgingival plaque from advanced marginal periodontitis in humans

Detection of stress proteins in Porphyromonas gingivalis and other oral bacteria by Western immunoblotting analysis

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