Rigid endomicroscopic system for multiphoton imaging and tissue ablation in the head and neck region

Lai, Chenting; Messerschmidt, Bernhard; Reichwald, Karl; Flämig, Sven; Eidam, Tino; Stutzki, Fabian; Calvarese, Matteo; Bae, Hyeonsoo; Meyer, Tobias; Schmitt, Michael; Groß, Herbert; Hoffmann, Franziska; Guntinas‐Lichius, Orlando

doi:10.1117/12.2650081

Cited by 1 publication

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“…7(c). The ablation region has been created by scanning the laser with a speed of 6.3 mm/s covering an area of 116 μm×116 μm 22 , 40 . After five consecutive scans on the focal plane, the sample was moved towards the probe tip with 4 μm steps, repeating the five scans procedure for each focal plane.…”

Section: Preliminary Endomicroscopic Imaging and Fs Laser Ablation Testsmentioning

confidence: 99%

“…The ablation region has been created by scanning the laser with a speed of

covering an area of

. 22 , 40 After five consecutive scans on the focal plane, the sample was moved towards the probe tip with

steps, repeating the five scans procedure for each focal plane. By moving the specimen toward the probe tip, the laser can be focused within the tissue, and the ablated volume can be increased.…”

Section: Preliminary Endomicroscopic Imaging and Fs Laser Ablation Testsmentioning

confidence: 99%

“…Some of the results in this article have been previously reported in SPIE conference proceedings. 22 , 23 , 40 The authors declare no commercial conflicts of interest related to this article.…”

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confidence: 99%

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Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

Lai,

Calvarese,

Reichwald

et al. 2023

J. Biomed. Opt.

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.SignificanceConventional diagnosis of laryngeal cancer is normally made by a combination of endoscopic examination, a subsequent biopsy, and histopathology, but this requires several days and unnecessary biopsies can increase pathologist workload. Nonlinear imaging implemented through endoscopy can shorten this diagnosis time, and localize the margin of the cancerous area with high resolution.AimDevelop a rigid endomicroscope for the head and neck region, aiming for in-vivo multimodal imaging with a large field of view (FOV) and tissue ablation.ApproachThree nonlinear imaging modalities, which are coherent anti-Stokes Raman scattering, two-photon excitation fluorescence, and second harmonic generation, as well as the single photon fluorescence of indocyanine green, are applied for multimodal endomicroscopic imaging. High-energy femtosecond laser pulses are transmitted for tissue ablation.ResultsThis endomicroscopic system consists of two major parts, one is the rigid endomicroscopic tube 250 mm in length and 6 mm in diameter, and the other is the scan-head (10 × 12 × 6 cm3 in size) for quasi-static scanning imaging. The final multimodal image accomplishes a maximum FOV up to 650 μm, and a resolution of 1 μm is achieved over 560 μm FOV. The optics can easily guide sub-picosecond pulses for ablation.ConclusionsThe system exhibits large potential for helping real-time tissue diagnosis in surgery, by providing histological tissue information with a large FOV and high resolution, label-free. By guiding high-energy fs laser pulses, the system is even able to remove suspicious tissue areas, as has been shown for thin tissue sections in this study.

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Section: Preliminary Endomicroscopic Imaging and Fs Laser Ablation Testsmentioning

confidence: 99%

“…The ablation region has been created by scanning the laser with a speed of

covering an area of

. 22 , 40 After five consecutive scans on the focal plane, the sample was moved towards the probe tip with

steps, repeating the five scans procedure for each focal plane. By moving the specimen toward the probe tip, the laser can be focused within the tissue, and the ablated volume can be increased.…”

Section: Preliminary Endomicroscopic Imaging and Fs Laser Ablation Testsmentioning

confidence: 99%

See 1 more Smart Citation

Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

Lai,

Calvarese,

Reichwald

et al. 2023

J. Biomed. Opt.

Self Cite

View full text Add to dashboard Cite

show abstract

Rigid endomicroscopic system for multiphoton imaging and tissue ablation in the head and neck region

Cited by 1 publication

References 8 publications

Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

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