Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins

Xie, Shaofeng; Švec, František; Fréchet, Jean M. J.

doi:10.1016/s0021-9673(97)00254-9

Cited by 146 publications

(55 citation statements)

References 20 publications

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“…The columns were used for the separation of polar aromatic compounds. Xie et al [32] prepared a porous poly(AAm-co-butyl methacrylate-co-N,N'-methylene-bis-AAm) monolithic column for hydrophobic interaction chromatography of proteins. The hydrophobicity of the monolithic column could be easily controlled by the percentage of hydrophobic monomer of butyl methacrylate (BMA) in the polymerization mixture.…”

Section: Acrylamide-based Monolithsmentioning

confidence: 99%

Monolithic media in microfluidic devices for proteomics

Nayak

Knapp

2006

Electrophoresis

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Considerable effort has been invested in the development of integrated microfluidic devices for fast and highly efficient proteomic studies. Among various fabrication techniques for the preparation of analytical components (separation columns, reactors, extractors, valves, etc.) in integrated microchips, in situ fabrication of monolithic media is receiving increasing attention. This is mainly due to the ease and simplicity of preparation of monolithic media and the availability of various precursors and chemistries. In addition, UV-initiated photopolymerization technique enables the incorporation of multiple analytical components into specified parts of a single microchip using photomasks. This review summarizes preparation methods for monolithic media and their application as microfluidic analytical components in microchips.

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Section: Acrylamide-based Monolithsmentioning

confidence: 99%

Monolithic media in microfluidic devices for proteomics

Nayak

Knapp

2006

Electrophoresis

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“…For example, macroporous polymers have been used as catalytic surfaces and supports, 165,166 separation and adsorbent media, [167][168][169] biomaterials, [170][171][172][173] and chromatographic materials. [174][175][176] Similarly macroporous ceramics can be employed as lightweight thermal and electrical insulators, as well as for catalysis. [177][178][179][180] Colloidal crystals are also of fundamental interest.…”

Section: Near-perfect Colloidal Crystalsmentioning

confidence: 99%

Entropically driven self-assembly of colloidal crystals on templates in space

Zimmerli

Yodh

2001

2001 Conference and Exhibit on International Space Station Utilization

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“…Thus, many kinds of monolithic stationary phases have been synthesized to meet various needs. [1][2][3] Several types of zwitterionic stationary phases were developed for ion chromatography. The zwitterionic structure has both anion and cation exchange sites.…”

Section: Introductionmentioning

confidence: 99%

“…Monolithic columns were introduced in the late 1990s; [1][2][3] many scientists have made attempts to synthesize zwitterionic monolith columns since then. Polymer-based monolithic columns were synthesized by in-situ polymerization.…”

Section: Introductionmentioning

confidence: 99%

Optimization and Investigation of Zwitterionic Monolithic Stationary Phases for Capillary Ion Chromatography

2017

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Zwitterionic monolithic columns were synthesized by a one-pot reaction using [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide, ethylene dimethacrylate, methanol and 2,2′-azobis(isobutyronitrile) as the monomer, cross-linker, porogen and initiator, respectively. The optimum conditions for polymerization and the efficiency of the prepared columns were examined for ion chromatography. The separation of five kinds of inorganic anions was achieved. The back pressures were monitored as increasing flow-rate, and the resulting plate heights (i.e. height equivalent of a theoretical plate, HETP) of SCN -were calculated at the inspected flow-rates. It was found that the increment rates of both the back pressure and HETP were rather slight. Mobile phases containing various cations or acid increased the retention times of the anions. Divalent cations could be separated, while monovalent cations could not be resolved due to their weak retention on the stationary phases.

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Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins

Cited by 146 publications

References 20 publications

Monolithic media in microfluidic devices for proteomics

Monolithic media in microfluidic devices for proteomics

Entropically driven self-assembly of colloidal crystals on templates in space

Optimization and Investigation of Zwitterionic Monolithic Stationary Phases for Capillary Ion Chromatography

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