RIP2 interacts with REL1 to control leaf architecture by modulating brassinosteroid signaling in rice

Zhang, Qiuxin; Liu, Guanming; Jin, Jing; Liang, Jiayan; Zhang, Jingjing; Peng, Haifeng; Wang, Wenyi; Zhang, Zemin

doi:10.1007/s00122-021-04011-w

Cited by 6 publications

(5 citation statements)

References 42 publications

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“…Exogenously applied AsA protects proteins and lipids against salinity- and drought-induced oxidative stress [ 31 , 35 ]. The rel1-D line has a strong resistance to drought when compared to the ZH11 line, and it exhibits markedly increased expression of ABA-related genes to promote the drought response [ 15 , 16 , 17 ]. In this study, we revealed that REL1 interacts with RIP5 in the chloroplasts.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we identified a rice T-DNA insertion gain-of-function mutation named ROLLED AND ERECT LEAF1 ( REL1 ) that was extensively involved in leaf morphology and abiotic-stress resistance, which had significantly increased expression under conditions of drought stress [ 14 ]. REL1 encodes an unknown functional domain that improves tolerance to drought stress by reducing ROS levels and inducing abscisic acid (ABA) responses [ 15 , 16 , 17 ]. In this study, we determined that REL1 physically interacts with RIP5 in the chloroplasts.…”

Section: Introductionmentioning

confidence: 99%

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RIP5 Interacts with REL1 and Negatively Regulates Drought Tolerance in Rice

Zhang,

He,

Zhang

et al. 2024

Cells

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Improving the drought resistance of rice is of great significance for expanding the planting area and improving the stable yield of rice. In our previous work, we found that ROLLED AND ERECT LEAF1 (REL1) protein promoted enhanced tolerance to drought stress by eliminating reactive oxygen species (ROS) levels and triggering the abscisic acid (ABA) response. However, the mechanism through which REL1 regulates drought tolerance by removing ROS is unclear. In this study, we identified REL1 interacting protein 5 (RIP5) and found that it directly combines with REL1 in the chloroplast. We found that RIP5 was strongly expressed in ZH11 under drought-stress conditions, and that the rip5-ko mutants significantly improved the tolerance of rice plants to drought, whereas overexpression of RIP5 resulted in greater susceptibility to drought. Further investigation suggested that RIP5 negatively regulated drought tolerance in rice by decreasing the content of ascorbic acid (AsA), thereby reducing ROS clearance. RNA sequencing showed that the knockout of RIP5 caused differential gene expression that is chiefly associated with ascorbate and aldarate metabolism. Furthermore, multiple experimental results suggest that REL1 is involved in regulating drought tolerance by inhibiting RIP5. Collectively, our findings reveal the importance of the inhibition of RIP5 by REL1 in affecting the rice’s response to drought stress. This work not only explains the drought tolerance mechanism of rice, but will also help to improve the drought tolerance of rice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RIP5 Interacts with REL1 and Negatively Regulates Drought Tolerance in Rice

Zhang,

He,

Zhang

et al. 2024

Cells

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“…Studies have shown that BR, as an efficient plant growth regulator, plays an important role in the development of leaf angle in monocotyledons 4 , 11 , 12 . The mutant LC1 was obtained from the T-DNA SHIP insert population, and phenotypic analysis revealed that cell elongation in the lobe is responsible for the enlarged lobe angle.…”

Section: Introductionmentioning

confidence: 99%

WGCNA analysis of the effect of exogenous BR on leaf angle of maize mutant lpa1

Ji,

Gao,

Zhuang

et al. 2024

Sci Rep

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Leaf angle, as one of the important agronomic traits of maize, can directly affect the planting density of maize, thereby affecting its yield. Here we used the ZmLPA1 gene mutant lpa1 to study maize leaf angle and found that the lpa1 leaf angle changed significantly under exogenous brassinosteroid (BR) treatment compared with WT (inbred line B73). Transcriptome sequencing of WT and lpa1 treated with different concentrations of exogenous BR showed that the differentially expressed genes were upregulated with auxin, cytokinin and brassinosteroid; Genes associated with abscisic acid are down-regulated. The differentially expressed genes in WT and lpa1 by weighted gene co-expression network analysis (WGCNA) yielded two gene modules associated with maize leaf angle change under exogenous BR treatment. The results provide a new theory for the regulation of maize leaf angle by lpa1 and exogenous BR.

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“…BRI1 is the starting point of the entire BR signaling pathway, acts as a sensor for BR, and the cascade transmits BR signaling [23]. Studies also observed that RIP2 encode a RING finger E3 ligase protein that directly binds to ROLLED AND ERECT LEAF 1 (REL1), a key regulator of leaf morphogenesis, which is broadly involved in leaf tilt by coordinating BR signaling [24]. However, the underlying molecular mechanism of BR governing the control of maize leaf angle remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-Based Weighted Correlation Network Analysis of Maize Leaf Angle Regulation by Exogenous Brassinosteroid

Gao

Zhuang

et al. 2022

Agronomy

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Maize (Zea mays L.) leaf angle is an important characteristic affecting high-density planting, and it is also a central indicator for maize plant type selection to improve yield. Brassinosteroids (BRs) are a class of phytohormones that could modulate the growth and development of plant leaf angles. However, its functional mechanism remains unclear in maize. In this study, we used maize self-line B73 as material to analyze the transcriptome of leaf cushion after BR treatment at the seedling stage. Using seven concentrations of exogenous BR-treated maize B73 plants, the results show that the leaf angle and the cell length near the leaf pillow increased and then decreased with BR concentration increasing, and the 50 μM level was the best treatment. Analysis of 11,487 differences expressed genes (DEGs) found that genes related to cell volume were up-regulated, and the expression of genes related to the cell division was down-regulated. It is speculated that exogenous BR regulates the size of the maize leaf angle by regulating cell volume and cell division, and so we constructed a molecular mechanism model of maize response to exogenous BR. The molecular mechanism model of exogenous BR through weighted gene co-expression network analysis (WGCNA) DEGs, and two gene modules related to changes in maize leaf angle were identified. The results can provide a theoretical basis for determining the mechanism of exogenous BR-regulated maize.

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RIP2 interacts with REL1 to control leaf architecture by modulating brassinosteroid signaling in rice

Cited by 6 publications

References 42 publications

RIP5 Interacts with REL1 and Negatively Regulates Drought Tolerance in Rice

RIP5 Interacts with REL1 and Negatively Regulates Drought Tolerance in Rice

WGCNA analysis of the effect of exogenous BR on leaf angle of maize mutant lpa1

Transcriptome-Based Weighted Correlation Network Analysis of Maize Leaf Angle Regulation by Exogenous Brassinosteroid

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