RIP3 expression induces a death profile change in U2OS osteosarcoma cells after 5-ALA-PDT

Coupienne, Isabelle; Fettweis, Grégory; Piette, Jacques

doi:10.1002/lsm.21088

Cited by 38 publications

(32 citation statements)

References 37 publications

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“…RIP3 has recently been outlined as a key gene involved in the regulation of apoptosis and necroptosis [13]. RIP3 was proved to play an important role in some malignant tumors, including hepatocarcinoma, chronic lymphocytic leukemia, osteosarcoma and glioblastoma [14][15][16][17]. However, the expression of RIP3 and its potential role in colorectal cancer remains elusive.…”

Section: Discussionmentioning

confidence: 99%

Receptor-interacting protein kinase 3 is a predictor of survival and plays a tumor suppressive role in colorectal cancer

Feng¹,

Song²,

Yu.Yu.³

et al. 2015

neo

142

114

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Receptor-interacting protein kinase 3 (RIP3) is a member of the RIP Ser/Thr kinase family, plays an important role in regulating cell survival, cell apoptosis and cell necrosis. However, the role of RIP3 in the carcinogenesis of colorectal cancer is still poorly understood.We used quantitative PCR and Western blot analysis to examine RIP3 expression in primary colorectal cancer and paired normal colorectal mucosa. RIP3 clinicopathological significance was assessed by immunohistochemical staining in 112 cases of primary colorectal cancer paired with noncancerous tissues. The biological function of RIP3 overexpression was measured by CCK8 assay and plate colony formation assay. Dual staining with fluorescent Annexin V and propidium iodide (PI) was used to discriminate apoptotic or necrotic cell death.RIP3 expression was significantly lower in colorectal cancer and associated with T stage, M stage and AJCC stage. Cox proportional hazard models showed that RIP3 expression was an independent prognostic factor for overall survival and disease-free survival in patients with colorectal cancer. Overexpression of RIP3 significantly suppressed the proliferation of colorectal cancer cells in vitro.Our results suggest that RIP3 may function as a novel prognostic indicator after surgery and play a suppressive role in the colorectal carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

Receptor-interacting protein kinase 3 is a predictor of survival and plays a tumor suppressive role in colorectal cancer

Feng¹,

Song²,

Yu.Yu.³

et al. 2015

neo

142

114

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“…Therefore we checked the influence of KD (Kinase Dead) and RHIM (RIP Homotypic Interacting Motif) RIP3 mutations, respectively abrogating the kinase activity of RIP3 and its ability to multimerize with RIP1 after PDT, as previously described [31]. By TSC2 immunoprecipitation, we showed that RIP3-KD mutant was poorly recruited to TSC2 while the RHIM mutant interaction with TSC2 was enhanced compared to RIP3-wt.…”

Section: Tsc2/rip3/14-3-3 Complex Stoichiometry Is Affected By Rip3 Kmentioning

confidence: 95%

“…Total lysates were obtained by resuspension of cells in total lysis buffer (50 mM TrisHCl pH 7.5, 150 mM NaCl, 1% Na-deoxycholate, 1% NP-40, 1 mM PMSF, Roche complete protease inhibitor and Halt™ Phosphatase Inhibitor cocktail (ThermoFisher)). The western blot procedure has been previously published [31].…”

Section: Western Blot Analysismentioning

confidence: 99%

RIP3 antagonizes a TSC2-mediated pro-survival pathway in glioblastoma cell death

Fettweis¹,

Valentin

L’homme³

et al. 2017

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Glioblastomas are the deadliest type of brain cancer and are frequently associated with poor prognosis and a high degree of recurrence despite removal by surgical resection and treatment by chemo-and radio-therapy. Photodynamic therapy (PDT) is a treatment well known to induce mainly necrotic and apoptotic cell death in solid tumors. 5-Aminolevulinic acid (5-ALA)-based PDT was recently shown to sensitize human glioblastoma cells (LN-18) to a RIP3 (Receptor Interacting Protein 3)-dependent cell death which is counter-acted by activation of autophagy. These promising results led us to investigate the pathways involved in cell death and survival mechanisms occurring in glioblastoma following PDT. In the present study, we describe a new TSC2 (Tuberous Sclerosis 2)-dependent survival pathway implicating MK2 (MAPKAPK2) kinase and 14-3-3 proteins which conducts to the activation of a pro-survival autophagy. Moreover, we characterized a new RIP3/TSC2 complex where RIP3 is suggested to promote cell death by targeting TSC2-dependent survival pathway. These results highlight (i) a new role of TSC2 to protect glioblastoma against PDT-induced cell death and (ii) TSC2 and 14-3-3 as new RIP3 partners.

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“…In many photodynamic responses, the inductor stimulus is 1 O 2 , originating from mitochondria, which enhances the formation of the receptor interacting protein 3 (RIP-3) complex. The cellular mechanism by which ROS generate such a response is not well understood [54,55]. …”

Section: Introductionmentioning

confidence: 99%

Cell Death Pathways and Phthalocyanine as an Efficient Agent for Photodynamic Cancer Therapy

Mfouo-Tynga

Abrahamse

2015

IJMS

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The mechanisms of cell death can be predetermined (programmed) or not and categorized into apoptotic, autophagic and necrotic pathways. The process of Hayflick limits completes the execution of death-related mechanisms. Reactive oxygen species (ROS) are associated with oxidative stress and subsequent cytodamage by oxidizing and degrading cell components. ROS are also involved in immune responses, where they stabilize and activate both hypoxia-inducible factors and phagocytic effectors. ROS production and presence enhance cytodamage and photodynamic-induced cell death. Photodynamic cancer therapy (PDT) uses non-toxic chemotherapeutic agents, photosensitizer (PS), to initiate a light-dependent and ROS-related cell death. Phthalocyanines (PCs) are third generation and stable PSs with improved photochemical abilities. They are effective inducers of cell death in various neoplastic models. The metallated PCs localize in critical cellular organelles and are better inducers of cell death than other previous generation PSs as they favor mainly apoptotic cell death events.

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RIP3 expression induces a death profile change in U2OS osteosarcoma cells after 5-ALA-PDT

Cited by 38 publications

References 37 publications

Receptor-interacting protein kinase 3 is a predictor of survival and plays a tumor suppressive role in colorectal cancer

Receptor-interacting protein kinase 3 is a predictor of survival and plays a tumor suppressive role in colorectal cancer

RIP3 antagonizes a TSC2-mediated pro-survival pathway in glioblastoma cell death

Cell Death Pathways and Phthalocyanine as an Efficient Agent for Photodynamic Cancer Therapy

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