Risk assessments in nanotoxicology: bioinformatics and computational approaches

Pikula, Konstantin; Zakharenko, Alexander M.; Чайка, Владимир; Kirichenko, Konstantin; Tsatsakis, Aristidis; Golokhvast, Kirill S.

doi:10.1016/j.cotox.2019.08.006

Cited by 32 publications

(13 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, in silico methods, such as bioinformatics and computational approaches, have become a comprehensive tool that allows one to evaluate the possible risks of NPs. Currently, nano-specific databases can be used to study NP risk assessments, such as the Online Chemical Modeling Environment (OCHEM), NanoDatabank, NanoHub, NanoMILE, and ModNanoTox [ 68 ]. To study NPs as new pharmaceutical drugs aimed at the prevention and treatment of diseases, the physico-chemical and structural properties of the nanomaterial must be investigated via in silico approaches.…”

Section: Category Of In Silico Modelsmentioning

confidence: 99%

Current Strategies in Assessment of Nanotoxicity: Alternatives to In Vivo Animal Testing

Huang

Lee

Hsu

et al. 2021

IJMS

View full text Add to dashboard Cite

Millions of experimental animals are widely used in the assessment of toxicological or biological effects of manufactured nanomaterials in medical technology. However, the animal consciousness has increased and become an issue for debate in recent years. Currently, the principle of the 3Rs (i.e., reduction, refinement, and replacement) is applied to ensure the more ethical application of humane animal research. In order to avoid unethical procedures, the strategy of alternatives to animal testing has been employed to overcome the drawbacks of animal experiments. This article provides current alternative strategies to replace or reduce the use of experimental animals in the assessment of nanotoxicity. The currently available alternative methods include in vitro and in silico approaches, which can be used as cost-effective approaches to meet the principle of the 3Rs. These methods are regarded as non-animal approaches and have been implemented in many countries for scientific purposes. The in vitro experiments related to nanotoxicity assays involve cell culture testing and tissue engineering, while the in silico methods refer to prediction using molecular docking, molecular dynamics simulations, and quantitative structure–activity relationship (QSAR) modeling. The commonly used novel cell-based methods and computational approaches have the potential to help minimize the use of experimental animals for nanomaterial toxicity assessments.

show abstract

Section: Category Of In Silico Modelsmentioning

confidence: 99%

Current Strategies in Assessment of Nanotoxicity: Alternatives to In Vivo Animal Testing

Huang

Lee

Hsu

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Despite intense interest in the problem of interaction between NPs and organisms and a relatively big amount of experimental data, the mechanisms of toxic action for NPs are still not fully clear [28][29][30]. Hence, the risk evaluation of nano-bio interaction, regulation, and development of the standards for safe production and utilization of NMs and NPs has become one of the high-priority problems in nanotechnology and nanotoxicology [31,32].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

et al. 2020

Self Cite

View full text Add to dashboard Cite

Nanoparticles (NPs) have various applications in medicine, cosmetics, optics, catalysis, environmental purification, and other areas nowadays. With an increasing annual production of NPs, the risks of their harmful influence to the environment and human health is rising. Currently, our knowledge about the mechanisms of interaction between NPs and living organisms is limited. Additionally, poor understanding of how physical and chemical characteristic and different conditions influence the toxicity of NPs restrict our attempts to develop the standards and regulations which might allow us to maintain safe living conditions. The marine species and their habitat environment are under continuous stress due to anthropogenic activities which result in the appearance of NPs in the aquatic environment. Our study aimed to evaluate and compare biochemical effects caused by the influence of different types of carbon nanotubes, carbon nanofibers, and silica nanotubes on four marine microalgae species. We evaluated the changes in growth-rate, esterase activity, membrane polarization, and size changes of microalgae cells using flow cytometry method. Our results demonstrated that toxic effects caused by the carbon nanotubes strongly correlated with the content of heavy metal impurities in the NPs. More hydrophobic carbon NPs with less ordered structure had a higher impact on the red microalgae P. purpureum because of higher adherence between the particles and mucous covering of the algae. Silica NPs caused significant inhibition of microalgae growth-rate predominantly produced by mechanical influence.

show abstract

“…Our research demonstrated that the sea urchin S. intermedius can be used in the testing of NPs. However, we believe that further investigations of NP environmental risk assessment should focus on the incorporation of multiomics approaches, predicting-toxicity tools, and the evaluation of the problems of biocorona formation and biotransformation of NPs by the organisms and in the media [ 55 , 56 , 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

Toxicity of Carbon, Silicon, and Metal-Based Nanoparticles to Sea Urchin Strongylocentrotus intermedius

et al. 2020

Self Cite

View full text Add to dashboard Cite

With the increasing annual production of nanoparticles (NPs), the risks of their harmful influence on the environment and human health are rising. However, our knowledge about the mechanisms of interaction between NPs and living organisms is limited. Prior studies have shown that echinoderms, and especially sea urchins, represent one of the most suitable models for risk assessment in environmental nanotoxicology. To the best of the authors’ knowledge, the sea urchin Strongylocentrotus intermedius has not been used for testing the toxicity of NPs. The present study was designed to determine the effect of 10 types of common NPs on spermatozoa activity, egg fertilization, and early stage of embryo development of the sea urchin S. intermedius. In this research, we used two types of multiwalled carbon nanotubes (CNT-1 and CNT-2), two types of carbon nanofibers (CNF-1 and CNF-2), two types of silicon nanotubes (SNT-1 and SNT-2), nanocrystals of cadmium and zinc sulfides (CdS and ZnS), gold NPs (Au), and titanium dioxide NPs (TiO2). The results of the embryotoxicity test showed the following trend in the toxicity level of used NPs: Au > SNT-2 > SNT-1 > CdS > ZnS > CNF-2 > CNF-1 > TiO2 > CNT-1 > CNT-2. This research confirmed that the sea urchin S. intermedius can be considered as a sensitive and stable test model in marine nanotoxicology.

show abstract

Risk assessments in nanotoxicology: bioinformatics and computational approaches

Cited by 32 publications

References 49 publications

Current Strategies in Assessment of Nanotoxicity: Alternatives to In Vivo Animal Testing

Current Strategies in Assessment of Nanotoxicity: Alternatives to In Vivo Animal Testing

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

Toxicity of Carbon, Silicon, and Metal-Based Nanoparticles to Sea Urchin Strongylocentrotus intermedius

Contact Info

Product

Resources

About