Risk Scoring System based on lncRNA Expression for Predicting Survival in Hepatocellular Carcinoma with Cirrhosis

Abstract

Objective: This study aims to explore the roles of long non-coding RNAs (lncRNAs) for predicting survival in hepatocellular carcinoma (HCC) patients with cirrhosis. Methods: A set of lncRNAs differentially expressed between HCC patients with or without cirrhosis was identified using expression profiles of The Cancer Genome Atlas database, and these lncRNAs were screened for their risk scoring system to predict recurrence-free survival (RFS) or overall survival (OS). Predictive ability of risk scoring systems was confirmed using uni- and multivariate Cox analyses while adjusting for clinical features. Predictive lncRNAs were analyzed by functional enrichment analysis. Results: Our screen identified 22 lncRNAs that were upregulated in the presence of cirrhosis and 59 that were downregulated. To predict OS of HCC patients with cirrhosis, a risk scoring system was developed with four lncRNAs (LINC02086, LINC00880, LINC01549 and AC136475.3); to predict RFS in these patients, the risk scoring system contained five lncRNAs (SH3RF3-AS1, AC104117.3, AC136475.3, LINC00239 and MRPL23-AS1). All risk scoring systems were associated with an area under the receiver operating characteristic curve > 0.7. Based on uni- and multivariate Cox analyses, the risk scoring system could serve as a significant independent predictor for OS in HCC patients with cirrhosis. Functional enrichment analysis suggested that the lncRNAs in the risk scoring systems are involved primarily in the pathway of Wnt signal and cytokine-cytokine receptor interaction. Conclusion: Risk scoring systems based on lncRNAs can effectively predict OS of HCC patients with cirrhosis. The system should be further developed and validated in larger, preferably multi-site patient populations.

Immune-related long non-coding RNAs can serve as prognostic biomarkers for clear cell renal cell carcinoma

“…Among these selected ILRPs, the roles of several irlncRNAs have previously been investigated in some cancers. Ye et al indicated that AC136475.3 can serve as a prognostic biomarker for patients with HCC who have cirrhosis (27). HOXB-AS3 has a dual role in various cancers; in colon cancer, it acts as a tumor suppressor, but in lung cancer, it acts as a tumor promoter (28,29).…”

<p>Long Non-Coding RNA LINC00239 Functions as a Competitive Endogenous RNA by Sponging microRNA-484 and Enhancing KLF12 Expression to Promote the Oncogenicity of Colorectal Cancer</p>

“… 21 Additionally, LINC00239 is identified as a biomarker to predict the overall survival of patients with hepatocellular carcinoma without fibrosis. 40 However, the expression and functions of LINC00239 in CRC are poorly understood. In this study, LINC00239 was found to be overexpressed in CRC tissues and cell lines.…”

Abnormal Expression of Mitochondrial Ribosomal Proteins and Their Encoding Genes with Cell Apoptosis and Diseases

“…In fact, there are numerous studies on the relationship between MRPs and digestive tract cancer, some of the explorations have been done in the last 5 years. As mentioned above, MRPL23 not only promotes the metastasis of salivary adenoid cystic carcinoma, it is also related to the occurrence of liver cancer [ 112 ], suggesting that the same MRP can affect different cancers. However, it is not clear whether there is a difference in the mechanism of its influence.…”