Ritkábban előforduló, alsó légúti fertőzést okozó                     Gram-negatív nem fermentáló pálcák

Juhász, Emese; Iván, Miklós; Pongrácz, Júlia; Kristóf, Katalin

doi:10.1556/650.2018.30926

Cited by 9 publications

(3 citation statements)

References 26 publications

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“…Many bacteria, such as Rhodopseudomonas sp., Acinetobacter sp., and Delftia sp. are often defective in hexokinase and phosphotransferase system and consequently cannot utilize glucose [80][81][82][83]. They are able to utilize a variety of organic acids including pyruvic acid.…”

Section: Discussionmentioning

confidence: 99%

Two-tiered mutualism improves survival and competitiveness of cross-feeding soil bacteria

Ge,

Zhai,

Xie

et al. 2023

The ISME Journal

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Metabolic cross-feeding is a pervasive microbial interaction type that affects community stability and functioning and directs carbon and energy flows. The mechanisms that underlie these interactions and their association with metal/metalloid biogeochemistry, however, remain poorly understood. Here, we identified two soil bacteria, Bacillus sp. BP-3 and Delftia sp. DT-2, that engage in a two-tiered mutualism. Strain BP-3 has low utilization ability of pyruvic acid while strain DT-2 lacks hexokinase, lacks a phosphotransferase system, and is defective in glucose utilization. When strain BP-3 is grown in isolation with glucose, it releases pyruvic acid to the environment resulting in acidification and eventual self-killing. However, when strain BP-3 is grown together with strain DT-2, strain DT-2 utilizes the released pyruvic acid to meet its energy requirements, consequently rescuing strain BP-3 from pyruvic acid-induced growth inhibition. The two bacteria further enhance their collective competitiveness against other microbes by using arsenic as a weapon. Strain DT-2 reduces relatively non-toxic methylarsenate [MAs(V)] to highly toxic methylarsenite [MAs(III)], which kills or suppresses competitors, while strain BP-3 detoxifies MAs(III) by methylation to non-toxic dimethylarsenate [DMAs(V)]. These two arsenic transformations are enhanced when strains DT-2 and BP-3 are grown together. The two strains, along with their close relatives, widely co-occur in soils and their abundances increase with the soil arsenic concentration. Our results reveal that these bacterial types employ a two-tiered mutualism to ensure their collective metabolic activity and maintain their ecological competitive against other soil microbes. These findings shed light on the intricateness of bacterial interactions and their roles in ecosystem functioning.

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Section: Discussionmentioning

confidence: 99%

Two-tiered mutualism improves survival and competitiveness of cross-feeding soil bacteria

Ge,

Zhai,

Xie

et al. 2023

The ISME Journal

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“…Since the emergence of carbapenem resistance among GNB some years ago, they have become one of the major causes of death among hospital-acquired infections (Juhász et al 2018). These organisms are also considered as public health threat worldwide (Tamma et al 2016;Illés et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Modified CIM test as a useful tool to detect carbapenemase activity among extensively drug-resistant Klebsiella pneumoniae, Escherichia coli and Acinetobacter baumannii

et al. 2021

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Purpose Timely detection of carbapenemases is essential for developing strategies to control the spread of infections by carbapenem-resistant isolates. The purpose of this study was to determine the epidemiology of carbapenemase genes among carbapenem-resistant isolates of Acinetobacter baumannii, Klebsiella pneumoniae, and Escherichia coli. In addition, the efficacy of the modified Hodge test (MHT), Carba NP test, and modified carbapenem inactivation method (mCIM) were compared. Methods A total of 122 carbapenem-resistant clinical isolates including 77 K. pneumoniae, 39 A. baumannii, and six E. coli were collected from hospitalized patients. Three phenotypic methods, including the MHT, Carba NP test, and mCIM were used for investigation of carbapenemase production. In addition, polymerase chain reaction (PCR) was performed to detect carbapenemase-encoding genes. Result The sensitivity and specificity of the MHT were 75.0% and 100%, respectively. In addition, Carba NP displayed 80.8% sensitivity and 100% specificity, whereas the sensitivity and specificity were 90.4% and 100% for the mCIM test, respectively. Among carbapenem-resistant isolates, 70, 84, and 87 isolates exhibited positive results according to the MHT, Carba NP test, and mCIM, respectively. PCR indicated the presence of one or more carbapenemase genes in 119 of carbapenem-resistant isolates, with blaKPC and blaVIM being the most commonly encountered. Co-production of ‘KPC and OXA-48’, ‘KPC and VIM’, and ‘KPC and IMP’ was detected in three, nine, and seven isolates, respectively. Conclusion Our results confirm that the mCIM test is a useful tool for the reliable detection of carbapenemase activity in enterobacterial isolates, especially in clinical microbiological laboratories with limited resources.

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“…Since the outbreak of CR-GNB some years ago, they have become one of the top causes of death among hospital-acquired infections [53]. This has raised concern about developing reliable and quick methods for detecting carbapenemases using phenotypic or genotypic approaches, which are a major driver of carbapenem resistance dissemination [54].…”

Section: Multidrug-resistantmentioning

confidence: 99%

Phenotypic and genotypic characterization of carbapenemases in carbapenem-resistant gram-negative bacilli isolated from adult cancer patients.

El.Nobi,

Elgendy,

Bakry

et al. 2023

Microbes and Infectious Diseases

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Background: Carbapenem-resistant Gram-negative bacteria (CR-GNB) infections are prevalent in cancer patients with weakened immune systems, causing significant morbidity and mortality. The empirical use of antimicrobials has reduced mortality but led to the emergence of multidrug-resistant (MDR) bacteria. In this study, identification and susceptibility testing were carried out using standard procedures (Kirby-Bauer and broth microdilution techniques), and phenotypic and genotypic detection of carbapenemase-producing GNB isolated from adult cancer patients was performed using conventional procedures. Methods: One hundred and eight Gramnegative bacteria were recovered from various specimens, with the most common isolates being, Escherichia (E.) coli (45; 41.7%), followed by Klebsiella spp. (38; 35.2%), Acromobacter spp. (9;8.3%), Acinetobacter (A.) baumannii (5; 4.6%) and others including Enterobacter aerogenes, Raoultella ornithinolytica, Serratia fonticola, Citrobacter brakii, Comamonas testosteroni, Proteus mirabilis (11; 10.2%). Concerningly, 64 of 108 Gram-negative bacterial isolates (59.3%) were MDR. Furthermore, 91 out of 108 GNB isolates (84.3%) revealed a pattern of meropenem resistance using the broth microdilution method, which is a worrying rise in the rate of carbapenem resistance. Following the modified carbapenem inactivation method (mCIM), EDTA carbapenem inactivation method (eCIM), and combined disc test as phenotypic tests for the preliminary screening of carbapenemase producers (CPs), conventional PCR was performed on the 91 extracted DNA (Using 6 common carbapenemase primers). Results: It was found that blaNDM was the most common 60(66%), then blaOXA-48, VIM 47 (51.6%), blaIMP 32(35.2%), blaKPC 20(22.2%), and blaGES 12(13.2%). Conclusion: Based on these results, rapid and precise carbapenemase detection is crucial for clinical care, epidemiological investigations, and infection control.

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Ritkábban előforduló, alsó légúti fertőzést okozó Gram-negatív nem fermentáló pálcák

Cited by 9 publications

References 26 publications

Two-tiered mutualism improves survival and competitiveness of cross-feeding soil bacteria

Two-tiered mutualism improves survival and competitiveness of cross-feeding soil bacteria

Modified CIM test as a useful tool to detect carbapenemase activity among extensively drug-resistant Klebsiella pneumoniae, Escherichia coli and Acinetobacter baumannii

Phenotypic and genotypic characterization of carbapenemases in carbapenem-resistant gram-negative bacilli isolated from adult cancer patients.

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