RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops

Rosa-Téllez, Sara; Kanhonou, Rodoldphe; Bellés, Carlos Castellote; Serrano, Ramón; Alepuz, Paula; Ros, Roc

doi:10.3390/agronomy10020250

Cited by 12 publications

(11 citation statements)

References 78 publications

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“…In sugar beet, six genes coding for RBPs were identified as being able to increase salt tolerance in yeast [ 146 ]. Two of these genes participate in splicing, and the other four have only been putatively assigned as being involved in RNA metabolism.…”

Section: Manipulation Of Rbps Confers Desirable Traits In Plantsmentioning

confidence: 99%

“…Two of these genes participate in splicing, and the other four have only been putatively assigned as being involved in RNA metabolism. One of these salt-tolerance genes, BvSATO1 (which is involved in splicing), was verified in sugar beet and Arabidopsis, which showed that BvSATO1 increased salt tolerance in Arabidopsis [ 146 ].…”

Section: Manipulation Of Rbps Confers Desirable Traits In Plantsmentioning

confidence: 99%

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The Landscape of RNA-Protein Interactions in Plants: Approaches and Current Status

Burjoski

Reddy

2021

IJMS

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RNAs transmit information from DNA to encode proteins that perform all cellular processes and regulate gene expression in multiple ways. From the time of synthesis to degradation, RNA molecules are associated with proteins called RNA-binding proteins (RBPs). The RBPs play diverse roles in many aspects of gene expression including pre-mRNA processing and post-transcriptional and translational regulation. In the last decade, the application of modern techniques to identify RNA–protein interactions with individual proteins, RNAs, and the whole transcriptome has led to the discovery of a hidden landscape of these interactions in plants. Global approaches such as RNA interactome capture (RIC) to identify proteins that bind protein-coding transcripts have led to the identification of close to 2000 putative RBPs in plants. Interestingly, many of these were found to be metabolic enzymes with no known canonical RNA-binding domains. Here, we review the methods used to analyze RNA–protein interactions in plants thus far and highlight the understanding of plant RNA–protein interactions these techniques have provided us. We also review some recent protein-centric, RNA-centric, and global approaches developed with non-plant systems and discuss their potential application to plants. We also provide an overview of results from classical studies of RNA–protein interaction in plants and discuss the significance of the increasingly evident ubiquity of RNA–protein interactions for the study of gene regulation and RNA biology in plants.

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Section: Manipulation Of Rbps Confers Desirable Traits In Plantsmentioning

confidence: 99%

Section: Manipulation Of Rbps Confers Desirable Traits In Plantsmentioning

confidence: 99%

The Landscape of RNA-Protein Interactions in Plants: Approaches and Current Status

Burjoski

Reddy

2021

IJMS

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“…Moreover, RBPs have been proposed as targets to improve stress tolerance in crops, e.g. a recent study showed that in halotolerant sugar beet (Beta vulgaris (Bv)) expression of BvSATO1, an RNA metabolism associated RBP, was repressed by salt treatment, while in Arabidopsis BvSATO1 increased salt tolerance [41].…”

Section: Drought Stress-induced Rna Interactome Changesmentioning

confidence: 99%

The increasing diversity and complexity of the RNA-binding protein repertoire in plants

Marondedze

2020

Proc. R. Soc. B.

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Post-transcriptional regulation has far-reaching implications on the fate of RNAs. It is gaining increasing momentum as a critical component in adjusting global cellular transcript levels during development and in response to environmental stresses. In this process, RNA-binding proteins (RBPs) are indispensable chaperones that naturally bind RNA via one or multiple globular RNA-binding domains (RBDs) changing the function or fate of the bound RNAs. Despite the technical challenges faced in plants in large-scale studies, several hundreds of these RBPs have been discovered and elucidated globally over the past few years. Recent discoveries have more than doubled the number of proteins implicated in RNA interaction, including identification of RBPs lacking classical RBDs. This review will discuss these new emerging classes of RBPs, focusing on the current state of the RBP repertoire in Arabidopsis thaliana , including the diverse functional roles derived from quantitative studies implicating RBPs in abiotic stress responses. Notably, this review highlights that 836 RBPs are enriched as Arabidopsis RBPs while 1865 can be classified as candidate RBPs. The review will also outline outstanding areas within this field that require addressing to advance our understanding and potential biotechnological applications of RBPs.

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“…The plant cDNA library from salt-stressed sugar beet leaves used in this study was previously described in the following references [55,56]. cDNA was ligated into the λPG15 phage and inserted into the pYPGE15 shuttle expression plasmid [57].…”

Section: Screening Of a Cdna Library From Nacl-induced Sugar Beet Leaves And Isolation Of Bvhb2mentioning

confidence: 99%

“…Seeds of B. vulgaris cv Dita [55,56] were sterilized for 3 min in pure ethanol and washed three times with sterile water and placed on sterile vermiculite at 25 • C to germinate. Ten-day-old seedlings were transferred to plastic pots containing 1 L of sterilized peat moss/vermiculite (1:1, v/v).…”

Section: Plant Materials and Growth Conditions For Sugar Beetmentioning

confidence: 99%

Overexpression of BvHb2, a Class 2 Non-Symbiotic Hemoglobin from Sugar Beet, Confers Drought-Induced Withering Resistance and Alters Iron Content in Tomato

et al. 2020

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Drought stress is one of the major threats to agriculture and concomitantly to food production. Tomato is one of the most important industrial crops, but its tolerance to water scarcity is very low. Traditional plant breeding has a limited margin to minimize this water requirement. In order to design novel biotechnological approaches to cope with this problem, we have screened a plant cDNA library from the halotolerant crop sugar beet (Beta vulgaris L.) for genes able to confer drought/osmotic stress tolerance to the yeast model system upon overexpression. We have identified the gene that encodes BvHb2, a class 2 non-symbiotic hemoglobin, which is present as a single copy in the sugar beet genome, expressed mainly in leaves and regulated by light and abiotic stress. We have evaluated its biotechnological potential in the model plant Arabidopsis thaliana and found that BvHb2 is able to confer drought and osmotic stress tolerance. We also generated transgenic lines of tomato (Solanum lycopersicum) overexpressing BvHb2 and found that the resulting plants are more resistant to drought-induce withering. In addition, transgenic lines overexpressing BvHb2 exhibit increased levels of iron content in leaves. Here, we show that class 2 non-symbiotic plant hemoglobins are targets to generate novel biotechnological crops tolerant to abiotic stress. The fact that these proteins are conserved in plants opens the possibility for using Non-GMO approaches, such as classical breeding, molecular breeding, or novel breeding techniques to increase drought tolerance using this protein as a target.

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RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops

Cited by 12 publications

References 78 publications

The Landscape of RNA-Protein Interactions in Plants: Approaches and Current Status

The Landscape of RNA-Protein Interactions in Plants: Approaches and Current Status

The increasing diversity and complexity of the RNA-binding protein repertoire in plants

Overexpression of BvHb2, a Class 2 Non-Symbiotic Hemoglobin from Sugar Beet, Confers Drought-Induced Withering Resistance and Alters Iron Content in Tomato

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