RNA-Binding Proteins Play an Important Role in the Prognosis of Patients With Testicular Germ Cell Tumor

Abstract

Testicular germ cell tumors (TGCTs) are common urological neoplasms in young adult males. The outcome of TGCT depends on pathologic type and tumor stage. RNA-binding proteins (RBPs) influence numerous cancers via post-transcriptional regulation. The prognostic importance of RBPs in TGCT has not been fully investigated. In this study, we set up a prognostic risk model of TGCT using six significantly differentially expressed RBPs, namely, TRMT61A, POLR2J, DIS3L2, IFIH1, IGHMBP2, and NPM2. The expression profiles were downloaded from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression datasets. We observed by performing least absolute shrinkage and selection operator (LASSO) regression analyses that in the training cohort, the expression of six RBPs was correlated with disease-free survival in patients with TGCT. We assessed the specificity and sensitivity of 1-, 3-, 5-, and 10-year survival status prediction using receiver operating characteristic curve analysis and successfully validated using the test cohorts, the entire TCGA cohort, and Gene Expression Omnibus (GEO) datasets. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and gene set enrichment analyses were carried out to seek the possible signaling pathways related with risk score. We also examined the association between the model based on six RBPs and different clinical characteristics. A nomogram was established for TGCT recurrence prediction. Consensus clustering analysis was carried out to identify the clusters of TGCT with different clinical outcomes. Ultimately, external validations of the six-gene risk score were performed by using the GSE3218 and GSE10783 datasets downloaded from the GEO database. In general, our study constructed a prognostic model based on six RBPs, which could serve as independent risk factor in TGCT, especially in seminoma, and might have brilliant clinical application value.

NPM2 in malignant peritoneal mesothelioma: from basic tumor biology to clinical medicine

“…At present, the research on the NPM family mainly focuses on NPM1, and there is a lack of related reports on NPM2. Only a few experiments have shown that the expression or deletion of NPM2 is related to the occurrence, development, prognosis, and outcome of tumors [ 23 – 26 ]. Therefore, the exploration of NPM2 may bring new breakthroughs in the field of MPM diagnosis and treatment.…”

Comprehensive transcriptomic analysis and machine learning reveal unique gene expression profiles in patients with immune‐mediated necrotizing myopathy

“…Moreover, abnormal expression of NDUFAF7 could lead to mitochondrial dysfunction that might contribute to the development of myositis, especially IMNM 23,24 . POLR2J is a DNA repair gene that, through post‐transcriptional regulation, affects a variety of cancers, including rectal tumors and testicular germ cell tumors 25,26 . CD99 is a transmembrane glycoprotein shown to be upregulated in various malignancies and is also crucial for peripheral immune responses.…”

“…23,24 POLR2J is a DNA repair gene that, through post-transcriptional regulation, affects a variety of cancers, including rectal tumors and testicular germ cell tumors. 25,26 CD99 is a transmembrane glycoprotein shown to be upregulated in various malignancies and is also crucial for peripheral immune responses. It regulates a step at or close to the end trans-endothelial migration of polymorphonuclear leukocytes 27 and present to be a promising therapeutic target in acute myeloid leukemia.…”

POLR2J is a potential biomarker for abnormal tumor progression, vorinostat sensitization, immune infiltration, and prognosis of glioblastoma multiform

“…Meanwhile, RPB3 is a crucial subunit of RNA polymerase II that can interact with ATF4 and enhance the activation of ATF4 by promoter recognition [11]. Interestingly, POLR2J is considered as a promising prognostic biomarker for patients with testicular germ cell tumor [12]. Meanwhile, POLR2J is also overexpressed in rectal tumor organoids compared with than normal rectum organoids [13].…”