RNA Biomarker Trends across Type I and Type II Aerobic Methanotrophs in Response to Methane Oxidation Rates and Transcriptome Response to Short-Term Methane and Oxygen Limitation in Methylomicrobium album BG8

Abstract

Methanotrophs are naturally occurring microorganisms capable of oxidizing methane, having an impact on global net methane emissions. Additionally, they have also gained interest for their biotechnological applications in single-cell protein production, biofuels, and bioplastics.

A methanotrophic bacterium to enable methane removal for climate mitigation

“…In some bacteria, strong transcriptional responses accompany growth under nutrient limitation and at low growth rates: Bacteria often decrease expression of the translation and transcription apparatus, up-regulate functions involved in motility and chemotaxis, and up-regulate amino acid synthesis pathways ( 25 , 29 – 31 ). To understand how M. buryatense 5GB1C responds to low methane at the transcriptional level, we quantified holistic gene expression of cultures grown at 500 ppm and 1,000 ppm at methane-limited steady-state in the bioreactor, with growth rates of 0.009 h −1 and 0.02 h −1 , respectively.…”

“…By contrast, many genes related to flagellar protein synthesis and chemotaxis are up-regulated ( Fig. 3 F ), as bacteria tend to be more active in searching for nutrients and more favorable environments under stress ( 29 ).…”

Ultrasound Induced Enhancement of Biological Gas-to-Liquid Process for Methanol Synthesis from Methane Using Methylotuvimicrobium buryatense 5GB1C

“…The pmoCAB operon expresses three protein subunits, viz ., PmoA, PmoB, and PmoC, which are directly related to the correct assembly and optimum activity of the pMMO enzyme to enhance the conversion of methane to methanol. The pmoA gene from the operon was studied to compare the expression levels in the ultrasound-treated and control (non-treated) samples. − As mentioned in a subsequent section, the methanotrophic cultures were sonicated at varying duty cycles for 10 h. The cultures were then subjected to mechanical shaking at 30 °C in the shaker incubator at an agitation speed of 200 rpm. The fermentation was terminated after 24 h, and samples were collected for total RNA extraction and pmoA gene expression analysis using the RNA polymerase gene, encoded by rpoD gene, as the housekeeping gene.…”

“…The pMMO expression enhanced by 1. 25 1. These strategies involve techniques like genomic engineering, use of methane vectors, bioreactor design modifications, and immobilization.…”

A novel nutritional induction strategy flexibly switching the biosynthesis of food-like products from methane by a methanotrophic bacterium

“…1). [11][12][13] Methylotuvimicrobium buryatense 5GB1 is a promising industrial microorganism for biomanufacturing due to its fast growth rate under haloalkaliphilic culture conditions and available genetic tools for synthetic biology. [14][15][16][17][18][19] Moreover, M. buryatense 5GB1 is tolerant to different degrees of methane sources in terms of concentration (500 ppm and lower) and composition (biogas or natural gas).…”