RNA‐directed DNA methylation requires an AGO4‐interacting member of the SPT5 elongation factor family

Bies‐Etheve, Natacha; Pontier, Dominique; Lahmy, Sylvie; Picart, Catherine; Véga, Danielle; Cooke, Richard; Lagrange, Thierry

doi:10.1038/embor.2009.31

Cited by 142 publications

(153 citation statements)

References 29 publications

(46 reference statements)

Supporting

Mentioning

145

Contrasting

Unclassified

Order By: Relevance

“…Mutational studies of these sites revealed that arginine methylation affects SPT5s association with RNA polymerase II and enhances the ability of SPT5 to mediate DRB-inhibited transcription (61). Thus, reduced arginine methylation of SPT5 affects its promoter association and transcriptional elongation.…”

Section: Identification Of Endogenous Arginine Mono-methylation (Mma)mentioning

confidence: 99%

Proteomic Analysis of Arginine Methylation Sites in Human Cells Reveals Dynamic Regulation During Transcriptional Arrest

Sylvestersen

Horn

Jungmichel

et al. 2014

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

The covalent attachment of methyl groups to the side-chain of arginine residues is known to play essential roles in regulation of transcription, protein function, and RNA metabolism. The specific N-methylation of arginine residues is catalyzed by a small family of gene products known as protein arginine methyltransferases; however, very little is known about which arginine residues become methylated on target substrates. Here we describe a proteomics methodology that combines single-step immunoenrichment of methylated peptides with high-resolution mass spectrometry to identify endogenous arginine mono-methylation (MMA) sites. We thereby identify 1027 site-specific MMA sites on 494 human proteins, discovering numerous novel mono-methylation targets and confirming the majority of currently known MMA substrates. Nuclear RNA-binding proteins involved in RNA processing, RNA localization, transcription, and chromatin remodeling are predominantly found modified with MMA. Despite this, MMA sites prominently are located outside RNA-binding domains as compared with the proteome-wide distribution of arginine residues. Quantification of arginine methylation in cells treated with Actinomycin D uncovers strong site-specific regulation of MMA sites during transcriptional arrest. Interestingly, several MMA sites are down-regulated after a few hours of transcriptional arrest. In contrast, the corresponding dimethylation or protein expression levels are not altered, confirming that MMA sites contain regulated functions on their own. Collectively, we present a site-specific MMA data set in human cells and demonstrate for the first time that MMA is a dynamic post-translational modification regulated during transcriptional arrest by a hitherto uncharacterized arginine demethylase. Molecular & Cellular Proteomics

show abstract

Section: Identification Of Endogenous Arginine Mono-methylation (Mma)mentioning

confidence: 99%

Proteomic Analysis of Arginine Methylation Sites in Human Cells Reveals Dynamic Regulation During Transcriptional Arrest

Sylvestersen

Horn

Jungmichel

et al. 2014

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

show abstract

“…The AGO4/siRNA complexes are recruited to target loci via base-pairing with scaffold transcripts generated by RNA polymerase Pol V (Pontier et al, 2005;Mosher et al, 2008;Wierzbicki et al, 2008Wierzbicki et al, , 2009). The recruitment of AGO4 to chromatin may also be facilitated by its interaction with the GW/WGrich extensions of NRPE1 (the largest subunit of Pol V) Pontes et al, 2006;El-Shami et al, 2007;Li et al, 2008) and KTF1/SPT5L (a transcription elongation factor) (Bies-Etheve et al, 2009;He et al, 2009). The conserved GW/WG motif, defining an "Ago hook," is widely present in factors implicated in AGO actions (Azevedo et al, 2011;Garcia et al, 2012;Pontier et al, 2012).…”

Section: Dna Methylationmentioning

confidence: 99%

RNAi in Plants: An Argonaute-Centered View

2016

View full text Add to dashboard Cite

Argonaute (AGO) family proteins are effectors of RNAi in eukaryotes. AGOs bind small RNAs and use them as guides to silence target genes or transposable elements at the transcriptional or posttranscriptional level. Eukaryotic AGO proteins share common structural and biochemical properties and function through conserved core mechanisms in RNAi pathways, yet plant AGOs have evolved specialized and diversified functions. This Review covers the general features of AGO proteins and highlights recent progress toward our understanding of the mechanisms and functions of plant AGOs.

show abstract

“…DsRNAs are synthesized by RDR2 using Pol IV transcripts as a template, processed into 24-nt siRNAs by DCL3, and loaded into AGO4 (Matzke et al 2009). AGO4 interacts with the Pol V subunit NUCLEAR RNA POLYMERASE E1 (NRPE1) possibly through a base-pairing interaction between AGO4-loaded siRNA and nascent Pol V transcripts (El-Shami et al 2007), which is aided by the SUPPRESSOR OF TY INSERTION 5-LIKE (SPT5L, also known as KOW domain-containing transcription factor 1; KTF1) (Bies-Etheve et al 2009). These RNAmediated protein interactions may be recognized by INVOLVED IN DE NOVO 2 (IDN2), which may recruit methylation machinery including DRMs to establish de novo methylation (Ausin et al 2009).…”

Section: Factors Involved In Rddm and Maintenance Of Dna Methylationmentioning

confidence: 99%

Induction of RNA-directed DNA methylation and heritable transcriptional gene silencing as a tool to engineer novel traits in plants

Kasai

Kanazawa

2013

Plant Biotechnology

View full text Add to dashboard Cite

Gene silencing through transcriptional repression can be induced by double-stranded RNA (dsRNA) that targets a gene promoter. This phenomenon, termed RNA-mediated transcriptional gene silencing (TGS), was first discovered in plants using a transgene that transcribes an inverted repeat of promoter sequence. However, endogenous genes differ from transgenes in the feasibility of TGS induction, by being more resistant to silencing. Heritable, transgenerational silencing of an endogenous gene has been induced by targeting dsRNA to the promoter in petunia and tomato plants, using a vector based on Cucumber mosaic virus. Efficient TGS depends on the function of a viral protein, which can facilitate epigenetic modifications through the transport of short interfering RNA to the nucleus. The efficiency of the TGS also depends on the length and nucleotide composition of the promoter RNA segments. Such epigenetic changes induced by the viral vector results in a novel class of modified plant, a plant that does not carry a transgene but has altered traits. Thus, TGS to modify the epigenetic state of a plant is now a feasible tool to engineer novel traits. Here we review epigenetic changes induced in a particular gene through RNA-directed DNA methylation and those induced randomly on the genome in terms of their use for plant biotechnology.

show abstract

RNA‐directed DNA methylation requires an AGO4‐interacting member of the SPT5 elongation factor family

Cited by 142 publications

References 29 publications

Proteomic Analysis of Arginine Methylation Sites in Human Cells Reveals Dynamic Regulation During Transcriptional Arrest

Proteomic Analysis of Arginine Methylation Sites in Human Cells Reveals Dynamic Regulation During Transcriptional Arrest

RNAi in Plants: An Argonaute-Centered View

Induction of RNA-directed DNA methylation and heritable transcriptional gene silencing as a tool to engineer novel traits in plants

Contact Info

Product

Resources

About