RNA interference in the termite Reticulitermes flavipes through ingestion of double-stranded RNA

Zhou, Xuguo; Wheeler, Marsha M.; Oi, Faith M.; Scharf, Michael E.

doi:10.1016/j.ibmb.2008.05.005

Cited by 202 publications

(158 citation statements)

References 55 publications

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“…housekeeping genes), genes with high mRNA turnover coding for proteins with short half-life, stability of dsRNA, and the ability to deliver sufficient amounts of dsRNA (Huvenne and Smagghe, 2010;Scott et al, 2013). The potential applications for RNAi in pest management include oral administration of dsRNA in baits (Zhou et al, 2008), embedded in nanoparticles (Zhang et al, 2010), expressed in E. coli and formulated as a biological insecticide (Zhu et al, 2011), or expressed in transgenic plants (Baum et al, 2007;Mao et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

et al. 2016

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“…Direct injection and oral delivery/feeding of dsRNA are the main methods used to initiate RNAi in insects, as demonstrated in Lepidoptera (Turner et al, 2006), Coleoptera (Araujo et al, 2006), Diptera (Misquitta and Paterson, 1999), Homoptera (Ghanim et al, 2007) and Hymenoptera (Zhou et al, 2008). Most of these studies have focused on the identification of gene function with regard to insect development (reviewed in Lü, 2008).…”

Section: Introductionmentioning

confidence: 99%

Using double-stranded RNA to explore the role of heat shock protein genes in heat tolerance in Bemisia tabaci (Gennadius)

Wan

2011

Journal of Experimental Biology

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SUMMARYThe whitefly, Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) biotype B, is one of the most destructive invasive pests of field and glasshouse crops, and has a high tolerance to heat. Our previous work found that whitefly females are more heat tolerant than males. In the present study, real-time PCR and double-stranded RNA (dsRNA) methods were used to explore the role of heat shock protein (Hsp) genes in whitefly of both sexes; this provided further evidence of the mechanism underlying the differential heat tolerance abilities of females and males. The results showed that both hsp23 and hsp70 mRNA expression levels were higher in females than in males from 37.5 to 42°C, while at the extreme temperature of 44°C the hsp70 mRNA level was higher in males than in females. There was no significant difference in hsp90 mRNA expression between females and males under heat shock conditions. Furthermore, the survival rate of females fed hsp23 or hsp70 dsRNA significantly decreased following heat shock at 44°C for 1h, but male survival rate was not significantly affected. Additionally, the survival rate of both females and males showed no significant change after they were fed with hsp90 dsRNA. Collectively, the present study shows that the optimum mRNA expression of Hsp genes in females promotes a higher survival rate under heat shock conditions; hsp23 and hsp70 play a key role for heat tolerance in females but not in males, and hsp90 shows no significant role in heat tolerance in either females or males. Further, our study indicates that feeding with dsRNA is an effective method by which to study gene function, and the simplicity of this approach opens the way for further research on gene function in different sexes and diverse groups of species.

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“…Oral delivery of dsRNA has enormous potential applications in the management of agricultural insect pests. Diet-mediated dsRNA-induced gene silencing has been previously evidenced in a wide variety of agricultural insect pests of the order Lepidoptera [46][47][48][49], Diptera [13,[50][51][52][53], Orthoptera [54], Hymenoptera [55], Hemiptera [15,[56][57][58][59][60][61][62][63][64][65], Coleoptera [63,[66][67][68][69], and Isoptera [16]. So far, dsRNA delivery to various insect has been accomplished through oral feeding, microinjection, soaking, and transgenics [12,70,71,46].…”

Section: Discussionmentioning

confidence: 99%

Non-Invasive Delivery of dsGST Is Lethal to the Sweet Potato Whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae)

Asokan

Rebijith

Roopa

et al. 2014

Appl Biochem Biotechnol

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The sweet potato whitefly, Bemisia tabaci (G.) biotype B (Hemiptera: Aleyrodidae), is one of the most economically important pest, by being a dreaded vector of Geminiviruses, and also causes direct damage to the crops by sucking phloem sap. Glutathione S-transferase (GST) is a large family of multifunctional enzymes that play pivotal roles in the detoxification of secondary allelochemical produced by the host plants and in insecticide resistance, thus regulates insect growth and development. The objective of this study is to show the potential of RNA interference (RNAi) in the management of B. tabaci. RNAi is a sequence-specific gene silencing mechanism induced by double-stranded RNA (dsRNA) which holds tremendous potential in pest management. In this regard, we sequenced the GST from B. tabaci and synthesized approximately 500-bp dsRNA from the above and delivered through diet to B. tabaci. Real-time quantitative PCR (RT-qPCR) showed that continuous application of dsGST at 1.0, 0.5, and 0.25 μg/μl reduced mRNA expression levels for BtGST by 77.43, 64.86, and 52.95 % which resulted in mortality by 77, 59, and 40 %, respectively, after 72 h of application. Disruption of BtGST expression will enable the development of novel strategies in pest management and functional analysis of vital genes in B. tabaci.

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RNA interference in the termite Reticulitermes flavipes through ingestion of double-stranded RNA

Cited by 202 publications

References 55 publications

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

Using double-stranded RNA to explore the role of heat shock protein genes in heat tolerance in Bemisia tabaci (Gennadius)

Non-Invasive Delivery of dsGST Is Lethal to the Sweet Potato Whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae)

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