RNA-Interference-Mediated miR-122-Based Gene Regulation in Colon Cancer, a Structural In Silico Analysis

Ganesan, Harsha; Nandy, Suman Kumar; Banerjee, Antara; Pathak, Surajit; Hong, Zhiwu; Sun, Xiao‐Feng

doi:10.3390/ijms232315257

Cited by 5 publications

(4 citation statements)

References 64 publications

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“…As there is a decline in TM cellularity in glaucoma due to apoptosis and ECM remodelling [ 167 , 168 ], AKT activation may play an important role in the protection of TM cellularity. In our datasets, up-regulated miRNAs including miR-29b-1-5p [ 169 ], miR-122-5p [ 170 , 171 ], miR-143-3p [ 172 ], miR-182-5p [ 173 , 174 ], miR-214-3p [ 175 ], and miR-708-5p [ 176 , 177 ] are all putative targets of AKT. PTEN indirectly influences cellular proliferation and apoptosis as a major negative regulator of the Akt signalling pathway [ 178 , 179 ].…”

Section: Discussionmentioning

confidence: 99%

The TGFβ Induced MicroRNAome of the Trabecular Meshwork

Doyle,

Callaghan,

Roodnat

et al. 2024

Cells

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Primary open-angle glaucoma (POAG) is a progressive optic neuropathy with a complex, multifactorial aetiology. Raised intraocular pressure (IOP) is the most important clinically modifiable risk factor for POAG. All current pharmacological agents target aqueous humour dynamics to lower IOP. Newer therapeutic agents are required as some patients with POAG show a limited therapeutic response or develop ocular and systemic side effects to topical medication. Elevated IOP in POAG results from cellular and molecular changes in the trabecular meshwork driven by increased levels of transforming growth factor β (TGFβ) in the anterior segment of the eye. Understanding how TGFβ affects both the structural and functional changes in the outflow pathway and IOP is required to develop new glaucoma therapies that target the molecular pathology in the trabecular meshwork. In this study, we evaluated the effects of TGF-β1 and -β2 treatment on miRNA expression in cultured human primary trabecular meshwork cells. Our findings are presented in terms of specific miRNAs (miRNA-centric), but given miRNAs work in networks to control cellular pathways and processes, a pathway-centric view of miRNA action is also reported. Evaluating TGFβ-responsive miRNA expression in trabecular meshwork cells will further our understanding of the important pathways and changes involved in the pathogenesis of glaucoma and could lead to the development of miRNAs as new therapeutic modalities in glaucoma.

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Section: Discussionmentioning

confidence: 99%

The TGFβ Induced MicroRNAome of the Trabecular Meshwork

Doyle,

Callaghan,

Roodnat

et al. 2024

Cells

View full text Add to dashboard Cite

show abstract

“…As there is a decline in TM cellularity in glaucoma due to apoptosis and ECM remodelling [177,178], AKT activation may play an important role in the protection of TM cellularity. In our datasets, upregulated miRNAs including miR-29b-1-5p [179], miR-122-5p [180,181], miR-143-3p [182], miR-182-5p [183,184], miR-214-3p [185] and miR-708-5p [186,187] are all putative targets of AKT. PTEN indirectly influences cellular proliferation and apoptosis as a major negative regulator of the Akt signalling pathway [188,189].…”

Section: Mirna Strandsmentioning

confidence: 99%

The TGFβ Induced MicroRNAome of the Trabecular Meshwork

Doyle,

Callaghan,

Roodnat

et al. 2024

Preprint

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Primary open-angle glaucoma (POAG) is a progressive optic neuropathy with a complex, multifactorial aetiology. Raised intraocular pressure (IOP) is the most important clinically modifiable risk factor for POAG. All current pharmacological agents target aqueous hu-mour dynamics to lower IOP. Newer therapeutic agents are required as some patients with POAG show a limited therapeutic response or develop ocular and systemic side ef-fects to topical medication. Elevated IOP in POAG results from cellular and molecular changes in the trabecular meshwork driven by increased levels of transforming growth factor β (TGFβ) in the anterior segment of the eye. Understanding how TGF affects both the structural and functional changes in the outflow pathway and IOP is required to de-velop new glaucoma therapies that target the molecular pathology in the trabecular meshwork. In this study we evaluated the effects of TGF-1 and -2 treatment on miRNA expression in cultured human primary trabecular meshwork cells. Our findings are pre-sented in terms of specific miRNAs (miRNA-centric), but given miRNAs work in net-works to control cellular pathways and processes, a pathway-centric view of miRNA ac-tion is also reported. Evaluating TGF-responsive miRNA expression in trabecular meshwork cells will further our understanding of the important pathways and changes involved in the pathogenesis of glaucoma and could lead to the development of miRNAs as new therapeutic modalities in glaucoma.

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“…HCT116 (colon cancer cells) and A549 (lung cancer cells) cell lines were purchased from the National Centre for Cell Science (NCCS), Pune, India. Both the cells are adherent cells and were cultured-as per the methods described by Ganesan et al (2022) and Jothimani et al (2022). The cells were expanded in standard DMEM (low glucose, Glutamax supplement) (Cat.…”

Section: Cell Line Culture Expansionmentioning

confidence: 99%

“…There are evident cases of Parkinson-like syndrome and Raynaud's Phenomenon, which occur due to extreme cytotoxicity of chemotherapeutics (Vogelzang et al, 1981). Further, some genes and microRNAs prove to be targets of gene silencing through RNA interference and might serve as effective therapeutic targets in understanding and treating colorectal cancer (Ganesan et al, 2022). Additionally, to vividly studying the cellular landscape of tumour microenvironment of colon cancer and lung cancer might aid in future perspectives for designing therapeutic regimens (Banerjee et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Anti-cancer Properties of Dietary Supplement CELNORM against Colon and Lung Cancer: An in vitro preliminary study

Mehta,

Dey,

Thakkar

et al. 2023

IJERR

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Cancer is a complex disease characterized by a cascade of events that culminate in the accumulation of several genetic alterations. Because of the high incidence and mortality rate, scientists began seeking novel medications that were harmful to cancer cells but not to healthy cells. This study aimed to investigate the effect of CELNORM and its various components individually in colon and lung cancer cells. Based on the LD50 value, colon and lung cancer cells were treated with two different dosages of curcumin, pepper, carrot and cucumber extracts, individually and CELNORM (formulation with all four ingredients). The cytotoxicity of each compound has been checked through morphological analysis and cell viability assay by using the CCK8 kit and statistically analyzed. qPCR-based Gene expression study has also been done to further validate the anti-cancerous properties of the compounds. Curcumin extract decreased cancer cell growth, as evidenced by cytotoxicity and morphological analyses, but pepper, carrot, and cucumber extracts showed a less significant reduction in cancer cell growth. CELNORM, on the other hand, had the highest significant effect in suppressing cell proliferation, indicating the novel health supplement CELNORM renders more efficacy than individual components. Gene expression analysis also verifies the anti-cancerous properties of CELNORM, though some gene expressions are not in the expected line. The morphological analysis and cell viability result has shown the efficacy of the CELNORM over the individual compounds of the CELNORM and gene expression analysis of proliferative genes such as Cyclin D, CDK6, PCNA and BCL-2 further validates the anti-cancerous properties of CELNORM and all four components.

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RNA-Interference-Mediated miR-122-Based Gene Regulation in Colon Cancer, a Structural In Silico Analysis

Cited by 5 publications

References 64 publications

The TGFβ Induced MicroRNAome of the Trabecular Meshwork

The TGFβ Induced MicroRNAome of the Trabecular Meshwork

The TGFβ Induced MicroRNAome of the Trabecular Meshwork

Anti-cancer Properties of Dietary Supplement CELNORM against Colon and Lung Cancer: An in vitro preliminary study

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