RNA interference of timeless gene does not disrupt circadian locomotor rhythms in the cricket Gryllus bimaculatus

Danbara, Yoshiki; Sakamoto, Tomoaki; Uryu, Outa; Tomioka, Kenji

doi:10.1016/j.jinsphys.2010.07.002

Cited by 48 publications

(67 citation statements)

References 37 publications

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“…aegypti ( Danbara et al 2010 , Kamae & Tomioka 2012 ) indicates that although the functional domains a reasonably similar, TIM may play distinct roles on the circadian clock of each species. Recent studies on the firebrat Thermobia domestica showed that Td’tim knockdowns (using dsRNA) abolished the locomotor rhythm in most of the treated firebrats for more than 30 days.…”

Section: Discussionmentioning

confidence: 99%

Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference

Gentile

Rivas

Lima

et al. 2013

Mem. Inst. Oswaldo Cruz

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Mosquitoes are the culprits of some of the most important vector borne diseases. A species’ potential as a vector is directly dependent on their pattern of behaviour, which is known to change according to the female’s physiological status such as whether the female is virgin/mated and unfed/blood-fed. However, the molecular mechanism triggered by and/or responsible for such modulations in behaviour is poorly understood. Clock genes are known to be responsible for the control of circadian behaviour in several species. Here we investigate the impact mating and blood-feeding have upon the expression of these genes in the mosquito Aedes aegypti . We show that blood intake, but not insemination, is responsible for the down-regulation of clock genes. Using RNA interference, we observe a slight reduction in the evening activity peak in the fourth day after dstim injection. These data suggest that, as in Drosophila , clock gene expression, circadian behaviour and environmental light regimens are interconnected in Ae. aegypti .

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Section: Discussionmentioning

confidence: 99%

Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference

Gentile

Rivas

Lima

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…Recent molecular studies have revealed that the cricket clock oscillates with cyclical expression of clock genes, as has been suggested for other insect species, but has some unique features as well (Moriyama et al, 2008;Danbara et al, 2010;Moriyama et al, 2012;Uryu et al, 2013). The cricket possesses multiple clocks that distribute both in the central nervous system and peripheral tissues and produce an appropriate temporal structure of an insect at large through mutual interactions Tomioka, 2010, 2014).…”

Section: Introductionmentioning

confidence: 84%

“…The RNAi of tim or cyc genes never abolished the locomotor rhythm but changed the free-running period; tim RNAi shortened and cyc RNAi lengthened it in DD (Danbara et al, 2010;Uryu et al, 2013). Quantitative PCR revealed that in tim RNAi crickets, mRNA levels of both tim and per were knocked down and no significant rhythm was observed in LD.…”

Section: Cricket's Oscillatory Mechanism: Central Pacemakermentioning

confidence: 98%

“…In the cricket G. bimaculatus, the clock machinery has been so far substantially clarified (Moriyama et al, 2008;Danbara et al, 2010;Moriyama et al, 2012;Uryu et al, 2013). It includes at least four clock genes, per, tim, Clk, and cyc (Fig.…”

Section: Cricket's Oscillatory Mechanism: Central Pacemakermentioning

confidence: 99%

“…In the cricket, we have shown that basically similar molecular constituents are involved in the clock machinery and some of them play a role similar to those in Drosophila clock but some may play different roles. Of particular interest is the mechanism through which the clock maintains its oscillation even after treated with tim or cyc dsRNA (Danbara et al, 2010;Uryu et al, 2013). Interestingly, Clk can oscillate in certain conditions , thus crickets have both Drosophila-type and mammalian-type oscillatory components.…”

Section: Future Perspectivesmentioning

confidence: 99%

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Post-Embryonic Development of Circadian Oscillations Within and Outside the Optic Lobe in the Cricket,Gryllus bimaculatus

Uryu

Tomioka

2014

Zoological Science

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Mutation of the clock gene timeless disturbs diapause induction and adult emergence rhythm in Helicoverpa armigera

Liu

Cai

Zhu

et al. 2023

Pest Management Science

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Background Circadian rhythms are physical and behavioral changes that follow the 24‐h cycle of Earth's light and temperature and are regulated by clock genes. Timeless (Tim) has been identified as a canonical clock gene in some insects, however, its functions have been little studied in lepidopteran pests. Results To investigate Tim (HaTim) gene function in Helicoverpa armigera, an important lepidopteran pest, we obtained the HaTim mutant using the CRISPR/Cas9 gene editing system. Our results showed that the transcript levels of HaTim rhythmically peaked at night in heads of the wild larvae and adult, and the diel expression of HaTim was sensitive to photoperiod and temperature. The expression rhythms of other clock genes, such as HaPer, HaCry1, HaCry2 and HaCwo, were disturbed in the HaTim mutant larvae, as that stage is a sensitivity period for diapause induction. Fifth‐instar wild‐type larvae could be induced to pupate in diapause under a short‐day photoperiod and low temperature, however, fifth‐instar HaTim mutant larvae could not be induced under the same conditions. In addition, the emergence of wild‐type adults peaked early at night, but the rhythm was disturbed in the HaTim mutant with arrhythmic expression of some clock genes, such as HaPer, HaCry1 and HaCwo in adults. Conclusion Our results suggest that the clock gene Tim is involved in diapause induction and adult emergence in H. armigera, and is a potential target gene for controlling pest. © 2023 Society of Chemical Industry.

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RNA interference of timeless gene does not disrupt circadian locomotor rhythms in the cricket Gryllus bimaculatus

Cited by 48 publications

References 37 publications

Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference

Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference

Post-Embryonic Development of Circadian Oscillations Within and Outside the Optic Lobe in the Cricket,Gryllus bimaculatus

Mutation of the clock gene timeless disturbs diapause induction and adult emergence rhythm in Helicoverpa armigera

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