RNA, Proteins and Polyamines During Tube Growth in Germinating Apple Pollen

Bagni, Nello; Adamo, Patrizia; Serafini-Fracassini, Donatella; Villanueva, Victor R.

doi:10.1104/pp.68.3.727

Cited by 72 publications

(41 citation statements)

References 17 publications

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“…Red Chief was collected from experimental plots (Dipartimento di Colture Arboree, Bologna, Italy; Azienda Agricola Rondinini, Faenza, Italy). Handling and storage were performed as described by Bagni et al [19].…”

Section: Plant Materialsmentioning

confidence: 99%

Effects of post-translational modifications catalysed by pollen transglutaminase on the functional properties of microtubules and actin filaments

Duca

Serafini-Fracassini

Bonner

et al. 2009

Biochemical Journal

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TGases (transglutaminases) are a class of calcium-dependent enzymes that catalyse the interactions between acyl acceptor glutamyl residues and amine donors, potentially making crosslinks between proteins. To assess the activity of apple (Malus domestica) pollen TGase on the functional properties of actin and tubulin, TGase was prepared from apple pollen by hydrophobic interaction chromatography and assayed on actin and tubulin purified from the same cell type. The enzyme catalysed the incorporation of putrescine in the cytoskeleton monomers. When tested on actin filaments, pollen TGase induced the formation of high-molecular-mass aggregates of actin. Use of fluoresceincadaverine showed that the labelled polyamine was incorporated into actin by pollen TGase, similar to with guinea pig liver TGase. The pollen TGase also reduced the enzyme activity and the binding of myosin to TGase-treated actin filaments. Polymerization of tubulin in the presence of pollen TGase also yielded the formation of high molecular mass aggregates. Furthermore, the pollen TGase also affected the binding of kinesin to microtubules and reduced the motility of microtubules along kinesincoated slides. These results indicate that the pollen TGase can control different properties of the pollen tube cytoskeleton (including the ability of actin and tubulin to assemble and their interaction with motor proteins) and consequently regulate the development of pollen tubes.

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Section: Plant Materialsmentioning

confidence: 99%

Effects of post-translational modifications catalysed by pollen transglutaminase on the functional properties of microtubules and actin filaments

Duca

Serafini-Fracassini

Bonner

et al. 2009

Biochemical Journal

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“…Accumulating evidence has showed that endogenous polyamine is important for pollen germination and pollen tube growth 78,92 . Bagni et al 6 reported that biosynthesis of polyamines took place before pollen tube emergence. MGBG reduced (at low concentration) or inhibited (at high concentration) pollen germination, which was promoted by Spd at low concentration 64 .…”

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confidence: 99%

Involvement of Polyamine in Floral and Fruit Development

Liu

Honda

Moriguchi

2006

JARQ

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Polyamines, including putrescine (Put), spermidine (Spd) and spermine (Spm), are polycationic compounds of low molecular weight that are widely distributed in living organisms. They are implicated in a variety of plant processes, such as floral and fruit development. In addition, correlation between polyamines and flower gender or fertility is reported in some plant species. Since polyamines, especially Spd and Spm share a common precursor, S-adenosylmethionine (SAM), with ethylene, they demonstrated competitive effects on functions in fruit development and ripening in many plants. Exogenous polyamines have been employed to regulate floral and fruit development and ripening in a lot of plants. Besides, polyamines have shown their potential for postharvest fruit management. Therefore, in this review, we focused on the involvement of polyamines in floral and fruit development along with perspectives of polyamine research.

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“…In those organs, Pas content has been shown to be related to the processes of cell proliferation, ovule differentiation, embryo sac formation, transcriptional and proteinlevel regulation during pollen-tube emergence and sexual differentiation (Bagni et al 1981;Biasi et al 1999;Song et al 2001). In the styles of 'Housui' Japanese pear (P. pyrifolia), an increase in Pas was observed 20 h after pollination, mainly owing to a significant putrescine increase after self-and cross-pollination; at the same time, an increase in ethylene production was reported (Franco-Mora et al 2005b).…”

Section: Introductionmentioning

confidence: 99%

Putrescine content of Japanese pear (Pyrus pyrifolia) styles increases in response to pollination

Franco-Mora

Tanabe

Tamura

2009

New Zealand Journal of Crop and Horticultural Science

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Although poly amines (Pas) are suggested to be involved in the pollination process, the kinetics of this process in Japanese pear (Pyruspyrifolia) are not currently understood. In this work, the content of putrescine increased (α 0.05) 5 h after in vitro pollination in styles of five different cultivars of Japanese pear. The evolution of spermidine and spermine within the cultivars used was not consistent; whereas spermidine contents decreased, increased, or did not have statistically significant changes (α 0.05), spermine content either decreased or increased. Moreover, the regulation of the ethylene and putrescine responses to pollination seemed to be linked to the cultivar's gynoecious genotype, and was influenced by the pollen source. These results agree with the idea that putrescine increases in the styles during the first few hours after pollination in this species.

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RNA, Proteins and Polyamines During Tube Growth in Germinating Apple Pollen

Abstract: ABSTRACT

Cited by 72 publications

References 17 publications

Effects of post-translational modifications catalysed by pollen transglutaminase on the functional properties of microtubules and actin filaments

Effects of post-translational modifications catalysed by pollen transglutaminase on the functional properties of microtubules and actin filaments

Involvement of Polyamine in Floral and Fruit Development

Putrescine content of Japanese pear (Pyrus pyrifolia) styles increases in response to pollination

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